Joko Sulistyo, Yati Sudaryati Soeka, Purnama Dewi
| Abstract views: 342 | PDF views: 694


An extracellular p-glucosidase[EC]derived from Asvereillus vulverulentus were separated and partially purified by successive chromatographies and its some characterization and transglucosylation capacity were studied.The purification protocol included precipitation with ammonium sulphate,gel filtration,ion exchange chromatography and native polyacrylamide gel electrophoresis.The enzyme readily hydrolysed cellobiose to form transglucosylation products in the present of primary alcohol acceptors.This P-glucosidase was stable at temperatures up to 70 °C and from pH 2.5 to 8.5, while its highest activity was in the pH 4.5 at 65°C.


Asaereillus pulverulentus. fl-glucosidase, purifikasi, transglukosilasi, glukosida.

Full Text:



Berka RM, Dunn-Coleman N and Ward M. 1992. Industrial enzymes from Aspergillus Species. In Bennet JW and Klich MA, Asversillus Biology and Industrial Applications. Butterworth- Heinemann, Boston.

Lowry OH, Rosebrough NJ, Fan* AL and Randall RJ. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry. 193, 265-270.

Garfin DE. 1990. One-Dimensional Gel Electrophoresis. In Deutscher MP, Guide to Protein Purification. Academic Press Inc., San Diego. P425-441.

Grover AK, Macmurchie DD and Cusley RJ. 1977. Studies on almond emulsin p-Dglucosidase: Isolation and characterization of bifunctional isoenzyme. Biochemical and Biophysics Ada. 482, 98-108,

Pridham JB. 1960. Hydrolytic enzymes. Biochemistry Journal. 76, 13-17.

Reese ET. Maguire, AH and Parrish FW. 1968 Glucosidases and exoglucanases. Canadian Journal of Biochemistry. 46, 25-34,

Sakama N and Tadasa K. 1995. Variation of inhibition modes by n-alcohols in arbutin (Hydroquinone-O-P-D-glucopyranoside) hydrolysis by P-glucosidase. Bioscience Biotechnology Bioengineering. 59, 1352-1354,

Shinoyama H, Kamiyama Y and Yasui T. 1988 Enzymatic synthesis of p-xylosides from xylobiose by application of the transxylosyl reaction of Aspergillus niger p-xylosidase. Agriculture Biological Chemistry. 52, 2197-2202.

Sulistyo J, Kamiyama Y, Ito H and Yasui T. 1994. Enzymatic synthesis of hydroquinone pxyloside from xylooligosaccharides. Bioscience Biotechnology Bioengineering. 7,1311-1313.

Sulistyo J, Kamiyama Y and Yasui T. 1995 Purification and some properties of Aspergillus pulverulentus p-xylosidase with transxylosylation capacity. Journal Fermentation Technology and Bioengineering. 1, 17-22.

Sulistyo J. 1996. Screening for p-xylosidases possessing transfer activity and identification of Transfer products. Jurnal Mikrobiologi Tropika. 1 (1), 7-12.

Sulistyo J. 1996. Formation of alkyl p-xylosides by enzymic transxylosylation. Jurnal Biologi Indonesia. 1, 12-18.

Sulistyo J. 1997. Production of partially purified axylosidase of Aspergillus pulverulentus. Jurnal Mikrobiologi Tropika. 1 (2), 76-83.


  • There are currently no refbacks.