AKTIVITAS ENZIM 2,4-D MONOOKSIGENASE DARI BERBAGAI MIKROBA

Nunik Sulistinah
10.14203/beritabiologi.v4i5.1242
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Abstract

Nine cultures both from fungi and bacteria have been selected for testing their 2,4-D monooxygenase activity to degrade 2,4-Dtohlorophenoxyacetic acid (2,4-D). The results showed that all the cultures which were tested grows at 1000 ppm 2,4-D. Three cultures (Trichoderma viride. Asperoillus niqer and Isolat E (unidentified)) of the nine cultures are able to grow at 4000 ppm. T. viride grows well on Minimal Basal Media which contained glucose and 2000 ppm 2,4-D and produced the highest biomass (0.8660 g/l) than the others. The biomass of T. viride grew on MBM (without glucose) and added with 2000 ppm 2,4-D is 0,6520g/l. This indicated that the culture is tolerant to 2,4-D and able to use 2,4-D compound as energy and carbon sources for its growth. But we failed to prove the 2,4-D monooxygenase activity of supernatant of T. viride by measuring the changing of pH-value in the 2,4-D breakdown reaction.

Keywords

Trichoderma viride, 2,4-D monooksigenase/2,4-D monooxygenase, 2,4-D (2,4-Dichlorophenoxyacetic acid), aromatik herbisida yang mengandung khlor/chlorinated aromatic herticide.

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References

Bell GR. 1957. Some Morphological and Biochemical Characteristic of a Soil Bacterium Which Decomposes 2,4- Dichlorophenoxyacetic Acid. Canadian Journal of Microbiology 3, 821-840

Beste CE (ed.). 1983. Herbicide Handbook of the Weed Science Society of America, 5th ed., p. 515. Weed Science Society of America, Champaign, III.

Clarkson WW, Yang CP and Harker AR. 1993. 2,4-D Degradation in Monoculture Biofilm Reactors. Water Resources 27', 1275-1284.

Daugherty DD and Karel SF. 1994. Degradation of 2,4-Dichlorophenoxyacetic Acid by Pseudomonas cepacia DBOI (pROlOl) in a Dual -SubtrateChemostat. Applied and Environmental Microbiology 60, 3261-3267.

Don RH and Pemberton JM. 1981. Properties of Six Pesticides Degradation Plasmids Isolated from Alcaligenes euthropus. Journal Bacteriology 145, 681-686.

Evans WC, Smith BSW, Fernley HN and Davies JL 1971. Bacterial Metabolism of 2,4- Dichlorophenoxyacetate. Biochemistry Journal 122, 543-551.

Fogarty MA and Tuovinen OH. 1991. Microbiological Degradation of Pesticides in Yard Waste Composting. Microbiological Reviews 55, 225-233.

Francis HC. 1995. Ground-Water Microbiology and Geochemistry. John Wiley & Sons Inc. 365-368.

Jensen HL and Peterson HI. 1952. Detoxification of Hormone Herbicides by Soil Bacteria. Nature (London) 170, 39-40.

Ka JO, Holben WE and Tiedje JM. 1994. Analysis of Competition in Soil among 2,4- Dichlorophenoxyacetic Acid-Degrading Bacteria. Applied and Environmental Microbiology 60, 1121-1128.

Loos MA, Roberts RN and Alexander M. 1967. Phenol as Intermediates in The Decomposition of Phenoxyacetates by an Arthrobacter species. Canadian Journal Microbiology 13, 377-385.

Martin J and Worthing CR (eds.). 1977. Pesticide Manual, 5th ed., p. 593. British Crop Protection Council, Thornton Heath, England

Narain RJP. 1992. Effects of Long-term 2,4-D Application on Microbial Populations and Biochemical Processes in Cultivated Soil. Biology and Fertility of Soils 13, 187-191.

Rogoff MH and Reid JJ. 1956. Bacterial Decomposition of 2,4-Dichlorophenoxyacetic Acid. Journal Bacteriology 71, 303-307.

Walker RL and Newman AS. 1956. Microbial Decomposition of 2,4-Dichloro-phenoxyacetic Acid. Applied and Environmental Microbiology 4,201-206.

Xing X-H, Yoshino T, Puspita NF and Unno H. 1995. Behavior of 2,4-Dichloro-phenoxyacetic Acid Degradation and Nitogen Conversion by an Activated Sludge. Biotechnology Letters 17, 335-340.

DOI: https://doi.org/10.14203/beritabiologi.v4i5.1242

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