ANALISIS VARIASI GENETIK Saccharomyces cerevisiae Dl TAHAN ETANOL DENGAN RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA)

Heddy Julistiono, Titin Yulineri, Sukamto Hanjono
10.14203/beritabiologi.v4i5.1240
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Abstract

Genetic variations among 3 cultures, which were treated with or without Mn of Saccharomvces cerevisiae D1, were analyzed using RAPD (Random Amplified Polymorphic DNA ) technique. The Mn-treatment of three cultures were as follows: the culture KMn was D1 strain, the culture Mn+ was D1 strain colony survived in ethanol 20%, which was previously treated with 0,5 mU MnSOt and the culture Mn- was a D1 colony survived in ethanol 20% without MnSOi treatment. Polymorphism of total DNA of the three cultures may indicate that mutation may occur in cells which were tolerant to ethanol. The locus or base change was not identified. However, since the oxygen uptake rate of the three cultures at catabolite derepression state were identical,
the results suggest that the locus may not be in mitochondrial DNA encoding respiratory chain proteins. The relation between DNA polimorphic and ethanol tolerant cell is still to be clarified.

Keywords

khamir/yeast, Saccharomvces cerevisiae, RAPD, variasi genetik/genetic variation, set tahan ethanol/ethanol tolerance cell.

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References

Costa V, Reis E, Quintanilha A and Moradas-Ferreira P. 1993. Acquisition of ethanol tolerance in Saccharomyces cerevisiae: the key role of the mitichondrial superoxide dismutase. Archives of Biochemistry and Biophysic 300, 608-614.

Costa V, Amorim MA, Reis E, Quintanilha A and Moradas-Ferreira P. 1997. Mitochondrial superoxide dismutase is essential for ethanol tolerance of Saccharomyces cerevisiae in the post-diauxic phase. Microbiology 143, 1649-1656.

Chance B, Sies H and Boveris A. 1979. Hydroperoxide Metabolism in Mammalian Organ. Physiological Review 59, 527-605.

De Winde JH, Thevelein JM, and Winderick J. 1997. From Feast to Famine: Adaptation to Nutrient Depletion in Yeast. In S. Hofmann and W.H. Mager : Yeast Stress Respons. Landes Bioscience USA, 7-52p.

Fukatsu T and Ishikawa H. 1996. Phylogenetic Position of Yeast-like Symbiont of Hamiltonaphis styraci (Homoptera, Aphididae) Based on 18S rDNA Sequence . Insect Biochemistry and Molecular Biology 26, 383-388

Gregory EM, Yost Jr FJ and Fridovich I. 1973. Superoxide Dismutase of Escherichia coli: Intracellular Localization and Functions. Journal of Bacteriology 115, 987-991.

Julistiono H dan Triana E. 1998. Peran Mn dalam proses fermentasi etanol pada khamir Saccharomyces cerevisiae D2 dan isolat indigenus T.K.H.M1.E3. Prosiding Seminar Nasional VI Persada Cabang Bogor, 15 Desember (in press).

McCord JM, Keele Jr BB and Fridovich I. 1971. An enzyme based theory of obligate anaerobiosis; the physiological role of superoxide dismutase. Proceeding of National Academy Science. United Stated of America 54, 887-891.

Messner R, Prillinger H, Altmann F, Lopandic K, Wimmer K, Molnar O and Weigang F. 1994. Molecular Charactarization and Random Amplified Polymorphic DNA Analysis of Mrakia and Sterigmatomyces Species. Internasional Journal of Systematic Bacteriology 44, 694-703.

Romano A, Casaregola S, Torre P, Gaillardin C. 1996. Use of RAPD and Mitochondrial DNA RFLP for Typing of Candida zeylanoides and Debaromyces hansenii Yeast Strain Isolated from Cheese. Syestematic and Applied Microbiology 19, 255-264.

Valenzuela A, Fernandez N, Fernandez V, Ugrate G and Videla LA. 1979. Effect of acute ethanol ingestion on lipoperoxidation and the activity of the enzymes related to peroxide metabolism in rat liver. Federation of European Biochemical Society Letter 111, 11-13.

Williams JG, Kubelik AR, Livak KJ, Ravalski JA and Tingey SV. 1990. DNA Polmorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 22, 6531-6535.

DOI: https://doi.org/10.14203/beritabiologi.v4i5.1240

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