PENGARUH INOKULASI SEJUMLAH ISOLAT AZOSPIRILLUM TERHADAP PERTUMBUHAN DAN SERAPAN PUPUK N PADA TANAMAN JAGUNG

S Gandanegara, S Slamet, Idawati Idawati, M Lina
| Abstract views: 319 | PDF views: 601

Abstract

Two greenhouse experiments had been conducted to study the effect of inoculation of some Azospirillum isolates on maize growth in sand culture and sterilized soil. In the first experiment some growth parameters were used, i.e., root length and root surface area, dry weight of root, shoot, and shoot length. Inoculation enhanced plant growth, as shown by increased of all plant growth parameters. Root length and root surface area were sensitive parameter and could be used for evaluating the effect of inoculation. The best plant perfomance achieved by inoculation with Azospirillum isolates Btl No. 8 and No. 11 and with the increase of plant biomass 24 and 53% over control, respectively. In the second experiment, six selected Azospirillum isolates were evaluated on growth, N uptake, and N contribution15 in maize plants. N isotope dilution method was used to measure the N contribution from fertilizer. Uninoculated control plants showed low dry weight and contribution from N fertilizer, 3.09 gram and 10.84%. Inoculation increased mineral nutrition expressed in higher N plant yield and N contribution from fertilizer. N plant yield ranging from 147 to 189 mg N as compared to 134 mg N in control plants. The high contribution of N fertilizer (13.21-14.35 %) achieved by inoculation with Azospirillum isolates No. 6, 7, 8, and RDCB Az 1

Keywords

Isolat Azospirillum/ Azospirillum isolate, tanaman jagung/corn, teknik N/ N technique, kontribusi N dari pupuk/N contribution from fertilizer

Full Text:

PDF

References

Arsac JF, Lamothe C, Mulard D and Fages J. 1990. Growth enhancement of maize (Zea mays L) through Azospirillum lipoferum inoculation : effect of plant genotype and bacterial concentration. Agronomie 10: 640-654.

Baldani VLD, Baldani JI and Dobereiner J. 1983. Effect of Azospirillum inoculation on root infection and nitrogen incorporation in wheat. Canadian Journal of Microbiology 29, 924-929.

Carley HE and Watson RD. 1966. A new gravimetric method for estimating root surface area. Soil Scence 102,289-291.

Cohen E, Okon Y, Kigel I, Noor I, and Henis Y. 1980. Increase in Dry Matter and N total in Zea mays and Setaria italica Associated with Nitrogen fixing/ Azospirillum spp. Plant Physiology 66, 746-749.

Del Gallo M and Fendrikl. 1994. The Rhizosphere and Azospirillum. Dalam : AzospirillumlPlant Association, Ed. by Y. Okon, Boca Raton, Fla. Him 57-75.

Dini Ariani. 2001. Report on the job-training in National Institute of Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan, 10 pages.

Fages J. 1994. Azospirillum inoculant and Field Experiment. Dalam: Azospirillum/ Plant Association, Ed. by Y. Okon, Boca Raton, Fla., Him 87-109.

Fallik E, Sarig S and Okon Y. 1994. Morphology and Physiology of Plant Roots Associated with Azospirillum, Dalam AzospiriWum/Plant Association, Ed. by Y. Okon, Boca Raton, Fla., Him. 77-86.

Hartmann A, Singh M and Klingmuller W. 1983. Isolation and characterization of Azospirillum mutants excreting high amounts of indole acetic acid. Canadian Journal of Microbiolog 29, 916.

Krieg N, Rand J, and Dobereiner J. 1984. Genus Azospirullum.Dalain: Bergey s Manual of Systematic Bacteriology. Vol. I. Edited by N. R. Krieg and J. E. Holt. Williams and Wilkins, Baltimore/ London. Him. 94-103.

Levanony H and Bashan Y. 1989. Enhancement of cell division in wheat root tips and growth of root elongation zone induced by Azospirillum brasilense Cd. Canadian Journal of Botany 67, 2216.

Sumner ME. 1990. Crop response to Azospirillum inoculation. Advance in Soil Science 12, 54.

TienTM, Gaskins MH, and Hubbell DH. 1979. Plant growth substance produced by Azospirillum brasilense and their effect on growth of pearl millet (Pennisetum americanum L.). Applied Environmental Microbiology 37, 1016.

Zapata, F. 1990. Isotope techniques in soil fertility and plant nutrition studies. Dalam: Use of Nuclear Techniques in Studies of Soil-Plant Relationship. Training Course Series No. 2, IAEA, Vienna, Him 61-127.


Refbacks

  • There are currently no refbacks.