ISOLASI, SELEKSI DAN OPTTMASI PRODUKSI PROTEASE DARIBEBERAPAISOLAT BAKTERI

Elidar Naiola, Nunuk Widhyastuti
10.14203/beritabiologi.v6i3.1219
| Abstract views: 3732 | PDF views: 5050

Abstract

Thirty-seven out of sixty-one bacterial isolates from various sources of samples were screened for protease production. The isolate of ISO PL3 could produce the highest enzyme activity, and it was used as a standard bacterial strain in this observation. For any reason,we implemented ISO PL2 to study the optimum condition for producing bacterial protease. Result shows that the maximum protease activity was obtained in a medium containing 100 gram of rice brand in a liter tofu liquid waste. The optimum for incubation was 4- 6 days (agitation of 130 rpm at room temperature) and pH 5.0 - 6.0. After cultivating on this liquid medium, the maximum protease activity of the 2 ISO PL3 was 113,52 x 10' U/ml. From the studies on morphological and physiological characterization, it was indicated that ISO PL3 resemble with the species Bacillus macerans.

Keywords

Produksi protease/Protease production. Bacillus macerans, isolasi/isolation, seleksi/selection, skrining/screened, karakterisasi/characterization

Full Text:

PDF

References

Aunstrup K, 1979. Production Isolation and Economic of Extracelluler Enzyme. Appl. Biochem andBioeng. Vol. 2. Academic.

Basuki W, 1997. Enzim dalam industri deterjen. Proceedings of The 1" Conference on Industrial Enzyme and Biotechnology, Jakarta, Him 206-213.

Bergmann M, 1942. A classification of proteolytic enzymes. Adv. Enzymol.

Cappuccino JG, 1983. Microbiology: A laboratory Manual. Addison-Wesley, USA.

Daniel ICW, 1979. Fermentation and Enzyme Technology. Jhon Wiley and Sons, New York.

Houston DF, 1972. Rice bran and polish. Dalam: Rice Chemistry and Technology, DF Houston (Ed). American Association of Cereal Chemists Inc., St Paul, Minnesota.

Mubarik NR, A. Suwanto dan MT Suhartono, 2000. Isolasi dan karakterisasi protease ektraseluler dari isolat bakteri termofilik ekstrim. Prosiding Seminar Nasional Industri Enzim dan Bioteknologi II. Mikrobniologi, Enzim dan Bioteknologi Dalam Perspektif Ekonomi dan Industri. Badan Pengkajian dan Penerapan Teknologi. Jakarta, Him 151-158.

Nurhayati, Ninik and Sumaryanto, 1997. Protease production by Bacillus substilis ATCC 1633. Proceeding The Is' Conferece on Industrial Enzyme and Biotechnology, Him 201-205.

Raharjo, 1995. Pemanfaatan Padatan Tersuspensi Dalam Limbah Tahu untuk Pakan Ternak. Dinas Perindustrian, Ujung Pandang, Him 5-6.

Suhartono MT, 2000. Eksplorasi protease bakteri asal Indonesia untuk aplikasi industri dan riset bioteknologi. Prosiding Seminar Nasional Industri Enzim dan Bioteknologi II. Him 125-133.

Thomas DB, 1984. A Textbook of Industrial Microbiology. Sinaver Associates, Sunderland, USA.

DOI: https://doi.org/10.14203/beritabiologi.v6i3.1219

Refbacks

  • There are currently no refbacks.