ISOLASI POPULASI DAN KARAKTERISASI BAKTERI Rhizobium PADA TANAH ASAL TAMAN NASIONAL GUNUNG HALIMUN

Sri Purwaningsih
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Abstract

The studies on the isolation of population and characterization of Rhizobium bacteria have been carried out in soil samples from Gunung Halimun National Park. West Java. Soil samples taken from rhizospheres of rasamala (Altingia exsetsa), puspa (Schima wallichii)and kianak (Castanopsis aciaunaassima).Isolation was done in YEMA (Yeast Extract Mannitol Agar) medium, and the population done with MPN (Most Probable Number) method. The growth characteristic was observed by using YEMA medium mixed respectively with Brom Thymol Blue and Congo Red as indicators.The population of Rhizobium bacteria in YEMA medium was in the range of 0.28-35 5CFU/g "' soil x 10 . The highest population of Rhizobium bacteria was found from the samples collected from soil with rasamala (Altingia excelsa) as the host plant Twent> four isolates were isolated, including nine isolates may be grouped as slow growing, while fifteen isolates

Keywords

Rhizobium. isolation, population, characterization, YEMA medium, soil, Gunung Halimun National Park.

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