Di Sela-Sela Laboratorium dan Plot Eksperimen MENGENAL HPLC: PERANANNYA DALAM ANALISA DAN PROSES ISOLASI BAHAN KIMIA ALAM

Tri Murningsih, Chairul Chairul
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Abstract

The development of method to explore natural chemical product initially from plants were started from the beginning and generously from time to time from the conventional one's until sophisticated equipments.The value of plants as food,cosmetic and medicines resources needs specific instruments and methods for determining the quantity as well as quality of the materials in plants.This paper describe in detail one of the sophisticated equipments commonly used for analyzing and isolating bioactive compounds the so called High Performance Liquid Chromatography (HPLC).

Keywords

HPLC/HPLC instrument, metode analisis/analysis method, metode isolasi/ isolation method, bahan kimia alam/ natural chemical product.

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References

Anonim. 1978. Chromatography in Pharmaceutical Chemistry, Manual for the Practising Chromatographer. E. Merck Darmstadt, 16-20.

Anonim. 1986. High Performance Liquid Chromatography and Accessories.

Beckman, 87 Arpino PJ and Guiochon. 1979. LC MS Coupling, AnaL Chem. 51, 683 A.

Blakeley CR and Vestal ML. 1983. Thermospray Interfase for Liquid Chromatography/Mass Spectrometriy, Anal. Chem. 55, 750.

Chairul. 2000. Socio-Economic Research Purposes (SERP-LIPI) Bahan Kimia dan Proses. Technical Service Office (TSOLIPI), Jakarta.

Covey YR, Lee ED, Bruins AP and Henion JD. 1986. Liquid Chromato-graphy/Mass Spectromentry. Anal. Chem. 55, 1451 A. Berita Biologi Volume 5, Nomor 2, Agustus 2000

Deyl Z, Macek K and Janak J. 1975. Liquid Column Chromatography. Elseviser Sci. Publ. Co. Oxford-New York USA, 3-10.

DPR RI. 1988. Garis Besar Huluan Negara (GBHN) 1988. Frei RW, Jansen H and Brinkman UA. 1985. Postcolumn Reaction Detectors for HPLC. Anal. Chem, 57, 1529A.

Glajch JL and Kirkland JJ. 1983. Optimization of Selectivity in Liquid Chromato-graphy. Anal. Chem. 55, 319A.

Gidding JC. 1965. Dynamic of Chromatography. Dekker, New York, Chap. 2 Harborne JB. 1987. Metode Fitokimia, Ed. II. ITB Bandung, 17-19.

Karger BL, Martin M and Guiochon G. 1974. Role of Column Parameter and Injection Volume on Detection Limits in Liquid Chromatograpgy. Anal. Chem. 46,1640. Knox JH. 1977. Practical Aspects of LC Theory. J. Chrom. Sci. 15, 352.

Loedin AA. 1999. Peran Riset Dalam Pendayagunaan Potensi Obat Tradisional Sebagai Unsur Dalam Sistem Pelayanan Kesehatan. Disampaikan Pada Seminar Nasional Pendayagunaan Potensi Obat Tradisional Indonesia Sebagai Unsur Dalam Sistem Pelayanan Kesehatan di Jakarta.

Martin M, Bill G, Eon C and Guiochon G. 1975. Optimization of Column Design and Operating Parameter in High Speed Liquid Chromatography. J. Chromatogr. ScL 12.438.

Roston D.A, Shoup RE and Kissinger PT. 1982. Liquid Chromatography/ Electrochemistry, Thin-Layer Multiple Electrode Detection. Anal. Chem. 54, 1417A.

Stahl E. 1987. Thin Layer Chromatography, A Laboratory Handbook 2nd. Springer- Verlag, Berlin.

Willard HH, Merritt LL (Jr), Dean JA, Settle FA (Jr). 1988. Instrumental Methods of Analysis 7th Ed. Wadworth. Inc. Bellmont, California USA, 580-613

Yang, L, Ferguson LG and Vestal ML. 1984. A New Transport Detector for High Pergormance Liquid Chromatography Based on Thermospray Vaporation. Anal. Chem. 56,2626.


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