Dyah Supriyati, Bambang Sunarko
| Abstract views: 269 | PDF views: 770


Our experiments showed that bacterial isolate TP was able to grow on acetonitrile, butyronitrile and propionitrile as sole sources of carbon, energy and nitrogen, but not on acrylonitrile dan benzonitrile. Besides on nitriles, isolate TP could grow on acetamide,propionamide, benzamide and nicotinamide, but not on acrylamide. However, none of the tested carboxylic acids could be used as growth subtrate for bacterial isolate TP. The best growth substrates of isolate TP were butyronitrile (CH3-CH2-CH2-CN) and propionamide (CH3-CH2-CONH2). When isolate TP grew on butyronitrile, the highest biomass concentration, the doubling-time (td), and the specific growth rate1(n) were 8.99 gram cell dry weight/liter, 4.8 h and 0.144 h , and when grew on propionamide were 4.57 gram cell dry weight/liter, 5.7 h and10.122 h", respectively.


Isolat Bakteri TP/bacterial isolate TP, senyawa nitril/nitrile compounds, degradasi/degradation, pertumbuhan/growth.

Full Text:



Asano Y, Tani Y and Yamad H. 1980. A New Enzyme "Nitrile Hydratase" which Degrades Acetonitrile in Combination with Amidase. Agric. Biol. Chem. 44 (9), 2251-2252.

Briglia M, Nakatsuka Y, Sugai T and Ohta H. 1996. Action of Nitrile Hydratase from Rhodococcus Rhodochrous IFO 15564 on Derivates of 2,5-Anhydro-D-Allonitrile. Biosci. Biotechnol.Biochem. 60 (9), 1540-1542.

DiGeronimo MJ and Antoine AD 1976. Metabolism of Acetonitrile and Propionitrile by Nocardia Rhodochrous LL100-21. Appl. Environ. Microbiol. 31(6), 900-906.

Langdahl BR, Bisp P and Ingvorsen K. 1996. Nitrile Hydrolysis by Rhodococcus Erythropolis BL1, an Acetonitrile- Tolerant Strain Isolated from A Marine Sediment. Microbiology 142,145-154.

Meyer O and Schlegel HG. 1983. Biology of Aerobic Carbon Monoxide Oxidizing Bacteria. Ann. Rev. Microbiol. 37, 277-310.

Meyer O, Sander A and Sunarko B. 1989. Verfahren und Biologisch aktive Substanzen zum Mikrobiologischen Abbau von Acetonitril in Mobilen Phasen der Hochdruck-Fluessigkeit-Chromatographie. German Patent No. 38 31 396

Nagasawa T, Nanaba H, Ryuno K, Takeuchi K,Yamada H. 1987. Nitrile Hydratase of Pseudomonas Chlororaphis B23. Eur. J.Biochem. 162,1305-1312.

O'Grady D and Pembroke JT . 1994. Isolation of a Novel Agrobacterium spp. Capable of Degrading a Range of Nitrile Compounds. Biotechnol. Lett. 16 (1), 47-50.

Pfennig N. 1974. Rhodopseudomonas globiformis sp.n., a New Species of the Rhodospirillaceae. Arch. Microbiol. 100, 197-206.

Purnomo D. 2000. Biokonversi Akrilonitril menjadi Akrilamida dan Asam Akrilat oleh Sel Corynebacterium D5. Skripsi. FMIPA, IPB.

Sulistinah N dan Sunarko B. 1999. Pertumbuhan Isolat Bakteri Dl pada berbagai Senyawa Nitril. J. Biol. Indonesia II (5), 250 -257.

Sunarko B. 1995. Mikrobieller Abbau von Acetonitril und Vinylacetat und Characterisier-ung von Vinylacetatesterase. Disertasi. Universitat Bayreuth.

Sunarko B. 1999. Biokonversi 3-Sianopiridin menjadi Nikotinamida dan Asam Nikotinat dengan Corynebacterium D5 sebagai Biokatalisator. KIPNAS VII, Serpong, 10 September 1999.

Supriyati D dan Sunarko B. 1999. Pertumbuhan berbagai Isolat Bakteri pada Propionitril. J.Mikrob. Trop. 2,25-31.

Wyatt JM and Linton EA. 1988. The Industrial Potential of Microbial Nitrile Biochemistry. Dalam: Cyanide Compo-unds in Biology. Mehnert, J and Brimer L (Eds.). John Wiley and Sons, Chichester. Him 32-42.


  • There are currently no refbacks.