Heddy Julistiono
| Abstract views: 329 | PDF views: 745


The effect of cryoprotectants glycerol and sucrose on cell viability and fermentation rate of Saccharomyces cerevisiae after freezing (-30 °C) and Chawing (30 °C) were studied.Both freezing and thawing were done rapidly. The mortality of cells treated with low concentrations of cryoprotectans (2.5, 5, and 10 %) after 15 and 30 days of cryopreservation, was remarkably higher than that of control and higher concentration (20% and 40%).Glycerol or sucrose with concentration of 20 % and 40 % protected cells from severe mortality only after 90 days of cryopreservation.Fermentation rate of cells treated with 20 % or 40 % of the two cryoprotectants were higher than that of control after 60 and 90 days of cryopreservation.The data indicated that in certain circumstance cryoprotectant could be toxic for the cells during freezing and thawing.Since biomembrane is not permeable to sucrose, therefore we proposed that target of sucrose toxicity may be extracellular, whereas glycerol, which penetrate cells,targets of the toxicity may be both extracellular and intracellular domains.Interaction between cryoprotectant and cell membranes is discussed.


Kerusakan oleh suhu rendah/freezing injury, krioprotektan/cryoprotectant, kriopreservasi/cryopreservation, khamir/ Saccharomyces cerevisiae.

Full Text:



Anchordoguy TJ, Rudolph AN, Carpenter JF, and Crowe JH. 1987. Modes of Interaction of Cryoprotectants with Membran Phospholipids during Freezing. Cryobiology 24,323-334.

Crowe JH, Crowe LM, and Chapman D. 1984. Preservation of Membranes in Anhydrobiotic Organism: The Role of Trehalox. Science 223, 701-703.

Dalgliesh. R.J. 1972. Changes in Hemolysis of Mammalian Erythrocytes by Rapid Freezing and Thawing by Mechanical Stress During their Permeation with Glycerol. Cryobiology 9, 277-282.

Doebler, GF. 1966. Cryoprotective Compounds. Review and Discussion of Structure and Function. Cryobiology 21,2-11.

Duzgunes N.1984. Dehydration and Phase Transition in Membrane Fusion. Cryobiology 21, 695-696.

Fahy GM. 1986. The Relevance of Cryoprotectant "toxicity" to Crybiology. Cryobiology 23,1-13.

Farrant J. 1973. The Effect of Salt or Various Cryoprotective Agent on Frog Sciativ Nerves. Cryobiology 23, 1-13

Leibo SP. 1976. Freezing Damage of Bovine Erythrocyte: Simulation using Glycerol Concentration Changes at Subzero Temperatures. Cryobiology 13, 387-598.

Ma E-P, Liu X-Z,. Liu M-DY, Han, Lui X and Wu ZrZ. 1998. The Effect of Superoxide Dismutase on the Recovery of Human Bone Marrow Hemopoiteic Stem Cells Stored at 4 °C. Cryobiology 37, 372-375.

Mazur P. 1970. Cryobiology: The Freezing of Biological System. Science (Washington D.C) 168, 939-940.

Mazur P and Schmidt JJ. 1968. Interaction of Cooling Velocity, Temperature, and Warming Velocity on The Survival of Frozen and Thawed Yeast. Cryobiology. 1:1-7

Meryman HT. 1971. Cryoprotective Agents. Cryobiology 8, 173-183.

Piracelli J and Pribor DB. 1972. Alteration of Semipermeabilty Properties of Red Cell by Slow Freezing. Cryobiology 9, 320-324.

Pribor DB. 1975. Biological Interaction Between Cell Membrane and Glycerol or DMSO. Cryobiology 12, 309-320.

Rail WF, P Mazur, and H Souzu. 1978. Physical- Chemical Basis of Protection of Slowly Frozen Human Erythrocites by Glycerol. Biophysic Journal 23,101-120.

Rammler DH. 1967. The Effect of DMSO on Several Enzyme Systems. Annual of New York Academic Science 141, 291-120.

Santarius KA and F Franks. 1998. Crypreservation of Lactate Dehydrogenase: Interactions amongs Various Cryoprotectants. Cryo-Letters 19, 37-48.

Strauss G, Schurternberger P, and Hauser H. 1986. The Interaction of Saccharides with Lipid Bilayer Vesicle: Stabilization During Freezing-Thawing and Freeze- Drying. Biochimica et Biophysca Ada 858, 169-180.

Takehara I and Rowe AW. 1971. Increase in ATPase Activity in Cell Red Membrane as Function of Freezing Regimen. Cryobiology 8, 559-565.


  • There are currently no refbacks.