Yadi Suryadi, M Machmud, MA Suhendar
| Abstract views: 295 | PDF views: 425


Raistonia solanacearum,the bacterial wilt pathogen, has a wide host range and genetic variability.Rapid and sensitive molecular techniques need to be developed for eariy detection and strain differentiation of the pathogen.Molecular techniques such as PCR and DNA hybridization have been succesfully used to detect and identify bacterial plant pathogens including R.solanacearum.These techniques were adopted under Indonesian condition, using purified and crude DNA from infected plant samples.An R.solanacearum specific DNA primer (OH/Y2) was used in the PCR test,and a DNA probe 5a67 were used in the non-radioactive hybridization test.The PCR techniqe could be used to detect R.solanacearum from infected plant samples in less than 5 hours.The DNA hybridization technique was applicable to differentiate strains ofR.solanacearum into three groups based on their DNA profiles.


deteksi dini/ early detection; Raistonia solanacearum; reaksi polimerasi berantai/ Polymerase Chain Reaction (PCR); hibridisasi DNA/ DNA hybridization; pembedaan strain/ strain differentiation.

Full Text:



Buddenhagen TW, L Sequeira and A Kelman. 1962. Designations of races in P. solanacearum. Phytopathology 52, 726.

Cook D, EE Barlow and L Sequeira. 1989. Genetic diversity of P. solanacearum: detection of restriction fragment length polymorphisms with DNA probes that specify virulence and hypersensitive response. Mol. Plant Microbe Interact. 2(3), 113-121.

Cook D, EE Barlow and L Sequeira. 1991. DNA probes as tools for the study of hostpathogen evolution: the example of P. solanacearum, pp. 103-108. In: H. Hanecke and D.P.S. Verma (eds.): Advances in molecular genetics of plant-microbe interactions. Vol 1. luwer Acad. Publ.

Dong LC, CW Sun, K Thies, DS Luthe, and CH Graves, Jr. 1992. Use of PCR to detect pathogenic strains of Agrobacterium. Phytopathology 82, 434-439.

Fahy FC and AC Hayward. 1983. Media and methods for isolation and diagnostic test, pp. 337-377. In F. C. Fahy and G. J. Persley (eds.) Plant bacterial disease. A diagnostic guide. Acad. Press, Sydney, p: 337-377.

Firrao G and R Locci. 1994. Identification of Clavibacter michiganensis subs. sepedonicus using the PCR. Can. J. Microbiol. 40, 148-151.

Gillings M, FC Fahy and C Davis. 1993. Restriction analysis of an amplified pg gene fragment differentiates strains of the phytopathogenic bacterium P. solanacearum. Letters in Appl. Microbiol. 17, 44-48.

Hanudin. 1993. Differentiation among biovar 3 isolates of P. solanacearum E.F. Smith using random amplified polymorphic DNA. ACIAR Report 1993, Canberra.

Hayward AC. 1991. Biology and epidemiology of bacterial wilt caused by P. solanacearum. Annu. Rev. Phytopathol. 29, 65 - 87.

Martin R, C Hover, S Grimme, C Grogan, J Holtke and C Kessler. 1990. A highly sensitive, non-radioactive DNA labelling and detection system. Biotechniques 9 (6), 762 - 768.

Miller SA and RR Martin. 1988. Molecular diagnosis of plant disease. Annu. Rev. Phytopathol. 26, 409 - 432.

Samadpour M, SL Moseley and S Lory. 1988. Biotinylated DNA probes for exotoxin A and pilin genes in the differentiation of P. aeruginosa strains. J. Clinic. Microbiol. 26(1), 2319-2323.

Sambrook J, FF Fritsch and T Maniatis. 1989. Molecular cloning, A Laboratory Manual. Sambrook J, FF Fritsch and T Maniatis. 1989. Molecular cloning, A Laboratory Manual.

Seal SE and JG Elphintone. 1992. Advances in identification and detection of P.solanacearum, p:35-57. In: A.C. Hayward and G.L. Hartman (eds.). Bacterial wilt, the disease and its causative agents, P. solanacearum. CAB-International, Walingford, UK.

Seal SE, LA Jackson, and MJ Daniels. 1992. Isolation of a P. solanacearum specific DNA probe by substractive hybridization and construction of species specific oligonucleotide primers for sensitive detection by the PCR. Appl. Environ. Microbiol. 58,3751-3758

Skoglund LG, SE Seal, JG Elphinstone and DE Berrios. 1993. Study of latent infection of potato tubers by P. solanacearum in Burundi. ACIAR Proceedings No.45, Canberra, Australia, p: 106-110.

Southern J. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503-517.

Suryadi Y dan M Machmud. 1997. Adopsi teknik PCR untuk pendeteksian P. solanacearum dan pengujian spesifisitas primer DNA. Proc. Kongres XIV dan Seminar Nasional PFI Vol II., Palembang, 27-29 Oktober 1997. Hal. 74-80

Venkitesh SR, RW Briddon and PG Markham. 1993. Detection of rice tungro bacilliform virus (RTBV) in asymptomatic leaves of tungro infected rice by Polymerase Chain Reaction (PCR). Int. Rice Res. Newsl. 18 (3), 13-14.

Woese CR. 1987. Detailed analysis of the higherorder structure of the 16S-like ribosomal nucleic acids Microbiol.Rev. 547, 621-669

Yabuuchi E, Y Kosako, I Yano, H Hotta and Y Nishiuchi. 1995. Transfer of two Burkholderia and an alcaligenes species to Ralstonia. gen. nov - proposal of R. picketii (Ralston, Palleroni and Doudoroff, 1973). comb.nov., R. solanacearum (Smith, 1896) comb.nov. and R. eutropha (Davis, 1969) comb.nov. Microbiol. Immunol. 39 (11),897-904.


  • There are currently no refbacks.