Hubungan Filogenetik Phrynella pulchra Boulenger, 1887 Berdasarkan Gen 16S rRNA

Farid Akhsani, Amir Hamidy, Achmad Farajallah, Eric N. Smith
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Abstract

Phrynella merupakan marga monotipik yang sejauh ini hanya terdiri dari satu jenis, yakni Phrynella pulchra. Jenis ini terdistribusi di Semenanjung Malaysia, Sumatra dan Pulau Mentawai. Kami mengevaluasi status taksonomi populasi yang berasal dari Sumatra menggunakan data sekuen dari gen 16S rRNA mitokondria. Hubungan filogenetik dianalisis menggunakan Neighbour Joining (NJ), Maximum Likelihood (ML), Bayesian Inference Analisys (BI) dan Unweighted Pair Group Method with Arithmetic Means (UPGMA). Hasil penelitian menunjukkan bahwa P. pulchra adalah kelompok monofiletik terhadap anggota luar (Metaphrynella, Kaloula dan Micryletta). Kelompok monofiletik P. pulchra terbagi menjadi dua kelompok besar: Sumatra dan Semenanjung Malaysia. Kelompok Sumatra terdiri dari dua subkelompok: Aceh dan Sumatra Utara- Bengkulu. Jarak genetik (uncorrected p-distance) antara populasi Semenanjung Malaysia dan Sumatra berkisar antara 1,1 sampai 2,0%, sedangkan di dalam populasi Sumatra berkisar antara 0,0 sampai 1,1%. Rendahnya jarak genetik populasi Semenanjung Malaysia dengan Sumatra secara taksonomi menunjukkan populasi tersebut masih berada pada tingkat Jenis yang sama.

Keywords

Mitokondria, populasi, jenis, Sumatra, taksonomi

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