Identifikasi Molekuler Microhyla, Tschudi 1839 dari Sumatera Berdasarkan Gen 16S rRNA

Tengku Gilang Pradana, Amir Hamidy, Achmad Farajallah, Eric N. Smith
| Abstract views: 102 | PDF views: 169

Abstract

Penelitian ini bertujuan mengevaluasi posisi jenis Microhyla dari Sumatra berdasarkan sekuen data dari gen 16S rRNA di dalam pohon filogeni Microhyla secara umum. Pohon filogeni dihasilkan dengan menggunakan analisis Unweighted Pair Group Method with Aritmatic Means (UPGMA), Neighbor Joining (NJ), Maximum Likelihood (ML) dan Bayesian Inference (BI). Kami menggunakan Phrynella pulchra, Kaloula pulchra, Kalophrynus pleurostigma, Chalcorana chal-conota, dan Leptobrachium waysepuntiense sebagai anggota outgroup. Hasil pohon filogeni menunjukkan bahwa Micro-hyla terdiri dari sembilan kelompok utama, enam diantaranya merupakan Microhyla dari Sumatra. Microhyla dari Su-matra mengelompok dengan M. achatina, kelompok M. heymonsi, kelompok M. fissipes, kelompok M. palmipes, kelompok M. berdmorei dan kelompok M. superciliaris. Beberapa kelompok memiliki jarak genetik yang besar seperti pada M. achatina (3,1−3,4%), M. heymonsi (3,3−7,1%) dan M. palmipes (2,5−3,4%). Microhyla fissipes membentuk parafiletik dengan M. mukhlesuri, kemungkinan termasuk kedalam complex atau cryptic spesies. Hal ini membutuhkan kajian lebih lanjut secara morfologi dan suara guna menentukan status taksonomi dari masing masing populasi di dalam jenis-jenis tersebut.

Keywords

jenis, kelompok, populasi, status, taksonomi

Full Text:

PDF

References

Das, I., Yaakob, N. & Sukumaran, J. (2007). A new species of Microhyla (Anura:Microhylidae) from the Malay Peninsula. Hamadryad, 31(2), 304–314.

Das, I. & Haas, A. (2010). New species of Microhyla from Sarawak: old world’s smallest frogs crawl out of miniature pitcher plants on Borneo (Amphibia: Anura: Microhylidae). Zootaxa, 2571, 37–52.

Duelmann, W.E. (1993). Amphibian species of the world: additions and corrections. Series: Special publication (University of Kansas. Museum of Natural History) no. 21. Lawrence: University of Kansas Publications.

Fouquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmell, N.J. (2007). Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses. PLoS ONE 2, 10, e1109.

Frost, D.R (Ed). (1985) Amphibian Species of the world: A Taxonomic and Geographical Reference. Lawrence: Allen Press.

Frost, D.R. (2016). Amphibian Species of the World: an Online Reference. Version 6.0 [Online]. Diambil dari http://research.amnh.org/herpetology/amphibia/index.html. American Museum of Natural History, New York, USA.

Zoo Indonesia 2017 26(2): 70-90

Identifikasi Molekuler Mircohyla Tschudi, 1839 dari Sumatra berdasarkan gen 16S rRNA Mitokondria

Hamidy, A., Matsui, M., Nishikawa, K. & Belabut, B.M. (2012). Detection of cryptic taxa in Leptobrachium nigrops (Amphibia, Anura, Megophryidae), with description of two new species. Zootaxa, 3398, 22−39.

Hamidy, A., Matsui, M., Shimada, T., Nishikawa, K., Yambun, P., Sudin, A., Kusrini, M.D. & Kurniati, H. (2011). Morphological and genetic discordance in two species of Bornean Leptobrachium (Amphibia, Anura, Megophridae). Molecular Phylogenetics and Evolution, 61(3), 904

Hasan, M., Islam, M.M., Kuramoto, M., Kurabayashi, A. & Sumida, M. (2014). Description of two new species of Microhyla (Anura: Microhylidae) from Bangladesh. Zootaxa, 3755(5), 401–418

Hasan, M., Islam, M.M., Khan, M.M.R., Igawa, T, Alam, M.S., Djong, H.T., Kurniawan, N., Joshy, H., Sen, Y.H., Belabut, D.M., Kurabayashi, A., Kuramoto, M. & Sumida, M. (2014). Genetic divergences of South and Southeast Asian frogs: a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya. Turkish Journal of Zoology, 38, 389–411

Hedges, S.B. (1994). Molecular evidence for the origin of birds. Proceedings of the National Academy of Science USA, 91, 2621–2624.

Hillis, D.M., Mable, B.K., Larson, A., Davis, S.K. & Zimmer, E.A. (1996). Nucleic acids IV: sequencing and cloning. Sunderland: Molecular Systematics Sinauer.

Howlader, M.S.A., Nair, A., Gopalan, S.V. & Merilä, J. (2015). A new species of Microhyla (Anura: Microhylidae) from Nilphamari, Bangladesh. PLoS ONE, 10(3), 1–18.

Huelsenbeck, J.P. & Hillis, D.M. (1993). Success of phylogenetic methods in the fourtaxoncase. Systematic Biology, 42, 247–264.

Huelsenbeck, J.P. & Ronquist, F.R. (2001). MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754–755.

Inger, R.F. (1999). Distribution of amphibians of the Ryukyu islands. Fieldiana Zoology, 32, 295–352.

Inger, R.F., Stuart, B.L & Iskandar, D.T. (2009). Systematics of a widespread Southeast Asian frog, Rana chalconota (Amphibia: Anura: Ranidae). Zoological Journal of the Linnean Society, 155, 123–147.

Jobb, G., von Haeseler, A. & Strimmer, K. (2004). Treefinder: a powerful graphical analysis environment for molecular phylogenetics. BMC Evolutionary Biology, 4, 18.

Khan, M.S. (2000) Buccopharyngeal morphology and feeding ecology of Microhyla ornata tadpoles. Asiatic Herpetological Research, 9, 130–138.

Identifikasi Molekuler Mircohyla Tschudi, 1839 dari Sumatra berdasarkan gen 16S rRNA Mitokondria

Tengku Gilang Pradana, Amir Hamidy, Achmad Farajallah, Eric N. Smith

Kurabayashi, A., Matsui, M., Daicus, B.M., Yong, H.S., Norhayati, A., Sudin, A., Kuramoto, M., Hamidy, A. & Sumida, M. (2011) From Antarctica or Asia? New colonization scenario for Australian-New Guinean narrow mouth toads suggested from the findings on a mysterious genus Gastrophrynoides. BMC Evolutionary Biology, 11, 175.

Kuraishi, N., Matsui, M., Hamidy, A., Belabut, D.M., Ahmad, N., Panha, S., Sudin, S., Yong, H.S., Jiang, J.P., Ota, H., Thong, H.T. & Nishikawa, K. (2012). Phylogenetic and taxonomic relationships of the Polypedates leucomystax complex (Amphibia). Zoologica Scripta, 42(1), 54–70.

Leaché, A.D. & Reeder, T.W. (2002). Molecu-lar systematics of the eastern fence lizard (Sceloporus undulatus): a com-parison of parsimony, likelihood, and Bayesian approaches. Systematics Biology, 51, 44–68.

Matsui, M., Ito, H., Shimada, T., Ota, H., Saidapur, S. K., Khonsue, W., Ueno, T.T. & Wu, G.F. (2005). Taxonomic relationships within the Pan-oriental narrow-mouth toad Microhyla ornata as revealed by mtDNA analysis (Amphibia, Anura, Microhylidae. Zoological Science, 22, 489–495.

Matsui, M., Panha, S., Khonsue, W. & Kuraishi, N. (2010). Two new species of the “kuhlii” complex of the genus Limnonectes from Thailand (Anura:

Dicroglossidae). Zootaxa, 2615, 1–22

Matsui, M. (2011). Taxonomic revision of one of the Old World’s smallest frogs, with description of a new Bornean Microhyla (Amphibia, Microhylidae). Zootaxa, 2814, 33–49.

Matsui, M., Hamidy, A., Belabut, D.M., Ahmad, N., Panha, S., Sudin, A., Khonsue, W., Oh, H.S., Yong, J.P., Jiang, J.P. & Nishikawa, K. (2011). Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy. Molecular Phylogenetics and Evolution, 61, 167–179.

Matsui, M., Hamidy, A. & Eto, K. (2013). Description of a New Species of Microhyla from Bali, Indonesia (Amfibia, Anura). Zootaxa, 3670(4), 579–590.

Peloso, P.L.V., Frost, D.R., Richards, S.J., Rodrigues, M.T., Donnellan, S., Matsui, M., Raxworthy, C.J., Biju, S.D., Lemmon, E.M. & Lemmon, A.R. & Wheeler, W.C. (2015). The impact of anchored phylogenomics and taxon sampling on phylogenetic inference in narrow-mouthed frogs (Anura, Microhylidae). Cladistics, 1, 1–28.

Sheridan, J.A., Bickford, D. & Yi Su, K.F. (2010). An examination of call and genetic variation in three wide-ranging southeast Asian anuran species. The Raffles Bulletin Of Zoology, 58(2), 369–379.

Zoo Indonesia 2017 26(2): 70-90

Identifikasi Molekuler Mircohyla Tschudi, 1839 dari Sumatra berdasarkan gen 16S rRNA Mitokondria

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 10, 2731–2739.

Tanabe, A.S. (2007). Kakusan: a computer program to automate the selection of a nucleotide substitution model and the configuration of a mixed model on multilocus data. Molecular Ecology Notes, 7, 962–964.

Vences, M., Thomas, M., Meijden, A., Chiari, Y. & Vietes, D.R. (2005). Comparative performance of the 16S rRNA gene in DNA barcoding of

amphibians. Frontiers in Zoology, 2(5), 1–12.

Vieth, M., Kosuch, J., Ohler, A. & Dubois, A. (2001). Systematics of Fejervarya limnocharis (Gravenhorst, 1829) (Amphibia, Anura, Ranidae) and related species. 2. Morphological and molecular variation in frogs from Greater Sunda island (Sumatra, Java, Borneo) with the definotion of two species. Alytes, 19, 5–28.

Zhao, E.M. (1999). Distribution patterns of amphibians in temperate East Asia. In: Duellman, W.E. (editor) Patterns of Distribution of Amphibians: A Global Perspective. Maryland: Johns Hopkins University Press, page. 421-423.


Refbacks

  • There are currently no refbacks.