Identifikasi Molekuler Microhyla, Tschudi 1839 dari Sumatera Berdasarkan Gen 16S rRNA

Tengku Gilang Pradana, Amir Hamidy, Achmad Farajallah, Eric N. Smith
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Penelitian ini bertujuan mengevaluasi posisi jenis Microhyla dari Sumatra berdasarkan sekuen data dari gen 16S rRNA di dalam pohon filogeni Microhyla secara umum. Pohon filogeni dihasilkan dengan menggunakan analisis Unweighted Pair Group Method with Aritmatic Means (UPGMA), Neighbor Joining (NJ), Maximum Likelihood (ML) dan Bayesian Inference (BI). Kami menggunakan Phrynella pulchra, Kaloula pulchra, Kalophrynus pleurostigma, Chalcorana chal-conota, dan Leptobrachium waysepuntiense sebagai anggota outgroup. Hasil pohon filogeni menunjukkan bahwa Micro-hyla terdiri dari sembilan kelompok utama, enam diantaranya merupakan Microhyla dari Sumatra. Microhyla dari Su-matra mengelompok dengan M. achatina, kelompok M. heymonsi, kelompok M. fissipes, kelompok M. palmipes, kelompok M. berdmorei dan kelompok M. superciliaris. Beberapa kelompok memiliki jarak genetik yang besar seperti pada M. achatina (3,1−3,4%), M. heymonsi (3,3−7,1%) dan M. palmipes (2,5−3,4%). Microhyla fissipes membentuk parafiletik dengan M. mukhlesuri, kemungkinan termasuk kedalam complex atau cryptic spesies. Hal ini membutuhkan kajian lebih lanjut secara morfologi dan suara guna menentukan status taksonomi dari masing masing populasi di dalam jenis-jenis tersebut.


jenis, kelompok, populasi, status, taksonomi

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