Analisis Gen Cytochrome Oxydase-1 (CO1) untuk Konfirmasi Status Taksonomi Burung Srigunting Sumbawa (Dicrurus, Dicruridae)

Dwi Astuti, Hidayat Ashari, Mohammad Irham, Sri Sulandari

Abstract

Sekuen DNA dari gen cytochrome oxydase-1 (COI) diadopsi untuk membangun pengelompokan dan kekerabatan dari burung-burung srigunting (Dicrurus; Dicruridae). Tujuannya adalah untuk mengkonfirmasi apakah srigunting dari Sum-bawa merupakan spesies tersendiri atau merupakan bagian dari superspesies D. hottentotus. Sekuen DNA sepanjang 795-bp pada gen COI diambil dari delapan burung srigunting dari Sulawesi Tenggara (D. hottentotus leucops), enam srigunting dari T.N. Gn. Halimun, Jawa Barat (D. remifer), dan dua srigunting dari Sumbawa. Analisis Neighbor-joining (NJ) dilakukan untuk mengkonstruksi pohon filogeninya dengan mengkalkulasi semua substitusi basa (transisi dan trans-verse) pada software MEGA-5. Pohon NJ menunjukkan bahwa dua individu yang berasal dari Sumbawa jelas-jelas terpisah dari Dicrurus asal Sulawesi Tenggara (D. hottentotus) dengan didukung nilai bootstrap sebesar 100 % dan dipisahkan oleh nilai divergensi sekuen > 3,5%. Sedangkan D. remifer memisah jauh dari keduanya. Penelitian ini cenderung mendukung pendapat bahwa srigunting dari Sumbawa merupakan populasi yang berbeda dan tidak termasuk dalam grup D. hottentottus, tetapi termasuk dalam grup D. densus dari Nusa Tenggara dan kemungkinan besar adalah spesies monopiletik tersendiri sebagai D. bimaensis.

Keywords

sekuen DNA, divergensi genetik, filogeni, Dicrurus

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References

Aliabadian, M., Beentjes K. K., Roselaar, C. S. K, van Brandwijk, H., Nijman, V. & Vonk, R. (2013). DNA barcoding of Dutch birds. R. ZooKeys, 365, 25-48.

Astuti, D. & Sulandari, S. (2010). The DNA sequence performance of COI gene in white cockatoos (Cacatua, Psittaci-formes). Treubia, 37, 1-14.

Barreira, A. S., Lijtmaer, D. A. & Tubaro, P. L. (2016). The multiple applications of DNA barcodes in avian evolution-ary studies. Genome, 59, 899–911.

Bilgin, R., Ebeo?lu, N., ?nak, S., K?rp?k, M. A., Horns, J. J., Cagan, H. & Seker-cioglu. (2016). DNA barcoding of birds at a migratory hotspot in Eastern Turkey highlights continental phylo-geographic relationships. PLOS ONE, 11 (6), e0154454. doi: 10.1371/journal.pone.0154454.

Campagna, L., St Clair, J. J. H., Lougheed, S. C., Woods, R. W., Imberti, S., & Tu-baro, P. L. (2012). Divergence be-tween passerine populations from the Malvinas – Falkland Islands and their continental counterparts: a compara-tive phylogeographical study. Biol. J. Linn. Soc. 106, 865–879.

Cicero & Jonson (2001). Higher-level phylog-eny of New World vireos (Aves: Vire-onidae) based on sequences of multi-ple mitochondrial DNA genes. Mo-lecular Phylogeny and Evolution, 20 (1), 27-40.

Coates, B. J. & Bishop, K. D. (2000). Panduan lapangan burung-burung di kawasan Wallacea: Sulawesi, Maluku dan Nusa Tenggara. BirdLife Inter-national. Indonesia Programme & Dove Publication. Indonesia. 246p

DeFilippis, V. R. & Moore, W. S. (2000). Resolution of phylogenetic relation-ships among recently evolved species as a function of amount of DNA se-quence: An empirical study based on Woodpeckers (Aves: Picidae). Molec-ular Phylogeny and Evolution, 16 ( 1), 143-160.

Dickinson, E. C. (eds). (2003). The Howard and Moore complete checklist of the birds of the world. London: Christo-pher Helm. 1039 p.

Eaton, J. A., van Balen, B., Brickle, N. W. & Rheindt, F. E. (2016). Birds of the Indonesian Archipeago. Greater Sun-das and Wallacea. Barcelona: Lynx Edicions.

Ericsson, P. G. P. & Johansson, U. S. (2003). Phylogeny of Passerida (Aves: Passer-iformes) based on nuclear and mito-chondrial sequence data. Molecular Phylogeny and Evolution 29, 126-138.

Hajibabaei, M., Singer, G. A. C , Hebert, P. D. N. & Hickey, D. A. (2007). DNA bar-coding: how it complements taxono-my, molecular phylogenetics, and population genetics. TRENDS in Genetics, 23 (4), 167- 172.

Hebert, P. D. N., Cywinska, A., Ball, S. L. & deWaad, R. (2003). Biological identi-fications through DNA bar-codes.Proceedings of the Royal Socie-ty B: Biological Science, 270: 313 - 321

Hebert, P. D. N.,Stoeckle,T. S., Zemlak, T. S. & Francis,C. (2004). Identification of birds through DNA barcodes.Public Library of Science. Biology 2: 1657- 1663

Huang, Z. H. & Ke, D. H. (2014). DNA bar-coding and evolutionary relationships of the Phasianidae family in China. Genetics and Molecular Research, 13 (3), 7411-7419.

Inskipp, T. Lindsey, N. Duckworth. 1996. An annotated checklist of the birds of the oriental region. United Kingdom: Oriental Bird Club.

Johnsen, A., Rindal E., Erikson P. G. P., Zuc-con, D., Kerr, K. C. R., Stoeckle, M. Y. & Lifjeld, J. T. (2010). DNA bar-coding of Scandinavian birds reveals divergent lineages in trans-Atlantic species. J Ornithol, 151, 565–578.

Kerr, K. C. R., Stoekle, M. Y., Dove, C. J., Weight, L. A., Franciss C. M. & Hebert, D. N. P. (2007). Comprehen-sive DNA barcode coverage of North American birds. Molecular Ecology, 7, 535-543.

Li, W. & Graur, D. (1991). Fundamental of molecular evolution. Sinauer Ass. Inc. Publisher, Sunderland, Massachusetts. 284 p .

Mayr, E. & Greenway, J. C. (eds). (1962). Check-list of birds of the world: A continuation of the work of James L. Peters. Cambridge : Museum of Com-parative Zoology.

Mindell, D. P., Sorenson, M. D., Dimcheff, D. E., Hasegawa, M., Ast J. C. & Yuri, T. (1999). Interordinal Relationships of Birds and Other Reptiles Based on Whole Mitochondrial genome. Syst. Biol., 48 (1), 138-152.

Nishiumi, I. (2012). DNA barcoding and spe-cies classification of Japanese birds. Japan Journal of Ornithology, 61, 223-237.

Park, H. Y., Yoo, H. S., Jung, G. & Kim, C. B. (2011). New DNA barcodes for identification of Korean birds. Genes Genom, 33, 2, 91. doi:10.1007/s13258- 010-0089-3.

Rocamora, G., Yeatman-Berthelot, D. & Bo-nan, A. (2017a). Drongos (Dicruridae). In: del Hoyo, J., Elliott, A., Sargatal, J., Christie, D.A. & de Juana, E. (eds.). Handbook of the Birds of the World Alive. Barcelona: Lynx Edicions. (retrieved from http://www.hbw.com/node/52360 on 28 February 2017).

Rocamora, G. & Yeatman-Berthelot, D. (2017b). Wallacean Drongo (Dicrurus densus). In: del Hoyo, J., Elliott, A., Sargatal, J., Christie, D.A. & de Juana, E. (eds.). Handbook of the Birds of the World Alive. Barcelona: Lynx Edi-cions, (retrieved from http://www.hbw.com/node/60581 on 2 March 2017).

Sibley, C. G. & Moonroe, B. L. Jr. (1990). Distribution and Taxonomy of Birds of the World. New Haven & London: Yale University Press.

Sukmantoro, W., Irham, M., Novarino, W., Hasudungan, F., Kemp, N. & Much-tar, M. (2007) Daftar Burung Indone-sia No. 2. Bogor: Indonesian Orni-thologists' Union.

Vaurie, C. (1949). A revision of the birds fam-ily Dicruridae. Bulletin of the Ameri-can Museum of Natural History, 93 (4), 199-342.

Weible, A.C. & Moore, S. (2002). Molecular phylogeny of cosmopolitan group of woodpeckers (Genus Picoides) based on COI and cyt b mitochondrial gene sequences. Molecular Phylogenetic and Evolution, 22 (1): 65 – 75.

Zuccon, D., Cibois, A., Pasquet, E., Ericson, P. G. P. (2006). Nuclear and Mito-chondrial Sequence Data Reveal the Major Lineages of Starling, Mynas and Related Taxa. Molecular Phyloge-ny and Evolution, 41, 333-344.

Zuccon, D., Pasquet, E. & Ericson, P.G.P. (2008). Phylogenetic Realationships among Palearctic-Oriental Starlings and Mynas (genera Sturnus and Acri-dotheres: Sturdidae). Zoologica Scripta, 37, 469-481.

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