MITOCHONDRIAL DNA VARIATIO OF THE RICE YELLOW STEM BORER, Scirpophaga incertulas, (LEPIDOPTERA: CRAMBIDAE) IN JAVA, INDONESIA

hari sutrisno
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Abstract

Scirpophaga incertulas is one of economically important rice pests in South East Asia, including Indonesia. Systematic investigation on biological characteristics of ecological races based on recently changed of agricultural practiced and environment has been conducted  in order to asses knowledge on genetic variation of population of S. incertulas in Indonesia. A 685 bp segment of mitochondrialDNA COII was amplified from 42 different yellow stem borer samples from five locations in Java. The objectives of this study were to generate mitochondrial CO II  sequences for all available yellow stem borer  samples and to define haplotypes and nucleotide sequence diversity of the different yellow stem borer populations. Six different haplotypes (YSB1, YSB2, YSB3, YSB4, YSB5 and YSB6) were identified in yellow stem borer populations. The majority of the sampled individuals caried haplotype YSB2. Overall, the results of Tajima’s test statistic indicated that the population of Java was D= 0.85201, which suggests that there was no indicative of purifying selection or there was no presence of deleterious mutation segregating in the population. However, the results were not significant (P> 0:10) and additional studies are required to confirm this finding.

Key words: Scirpophaga incertulas, yellow stem borer, MitochondrialDNA COII

 

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References

Amir, M., A. Kartohardjono, S. Siwi & R. Ubaidillah 2004. Morphological species variability in the stem borer genus (Lepidoptera: Pyralidae) on graminous crops. Treubia 33: 147-163.

Chen, M., A. Shelton & G.Y. Ye 2011. Insect-resistant genetically modified rice in China: from research to commercialization. Annual Review of Entomology 56: 81-101. doi: 10.1146/annurev-ento-120709-144810.

Deka, S. & S. Barthakur 2010. Overview on current status of biotechnological interventions on yellow stem borer Scirpophaga incertulas (Lepidoptera: Crambidae) resistance in rice. Biotechnology Advance 28 (1): 70-81. doi: 10.1016/j.biotechadv.2009.09.003.

Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for window 95/98/NT. Nucleic Acid Symposium Series 41: 95-98.

Irwin, D.M., T.D. Kocher & A.C. Wilson 1991. Evolution of the cytochrome b gene of mammals. Journal of Molecular Evolutionary 32: 128-144.

Kimura M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. Journal of Molecular Evolutionary 16: 111-120.

Kranthi, S., K.R. Kranthi, A.A. Bharose, S.N. Syed, C.S. Dhawad, R.M. Wadaskar & E.K. Patil 2006. Cytochrome oxidase I sequence of Helicoverpa (Noctuidae: Lepidoptera) species in India—its utility as a molecular tool. Indian Journal of Biotechnology 5: 195-199.

Kumar, L.S., A.S. Sawant, V.S. Gupta & P.K. Ranjekar 2001. Genetic variation in Indian populations of Scirpophaga incertulas as revealed by RAPD-PCR analysis. Biochemical Genetics 39 (2): 43-57.

Lange, C.L., K. D. Scot, G. C. Graham, M. N. Sallam, P. G. Allsopp 2004. Sugarcane moth borers (Lepidoptera: Noctuidae and Pyralidae): phylogenetic constructing using CO II and 16 S mitochondrial partial gene sequence. Bulletin of Entomological Research 94: 457-464.

Lewvanich, A. 1982. A revision of the Old World species of Scirpophaga (Lepidoptera: Pyralidae). Bulletin of the British Museum. (Entomology) Ser 42: 185-298.

Librado, P. & J. Rozas 2009. DnaSPv5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25: 1451-1452.

Liu, H & A. T. Beckenbach 1992. Evolution of the mitochondrial cytochrome oxidase II gene among 10 orders of insects. Molecular Phylogenetic and Evolution 1: 41-52.

Mastrangelo, T., D. Paulo, L.W. Bergamo, E.G.F. Morais, M. Silva, G. Bezerra-Silva & A.M.C. Azeredo-Espin 2014. Detection and genetic diversity of a Heliothine invader (Lepidoptera: Noctuidae) from North and North East of Brazil. Journal of Economic Entomology 107 (3): 1-11.

Raffiudin, R., R.M. Winnie & I.M. Samudra 2011. Intraspecific variations of 16s mitochondrial gene sequences of yellow rice stem borer, Scirpophaga incertulas (Lepidoptera: Crambidae) from West Java. BIOTROPIA 18 (1): 24 – 30.

Roe, A.D. & F.H. Sperling 2007. Patterns of evolution of mitochondrial cytochrome c oxidase I and II DNA and implications for DNA barcoding. Molecular Phylogenetics and Evolution 44: 325- 345. doi: 10.1016/j.ympev.2006.12.005.

Simon, C., F. Frati, A. Beckenbach, B. Crespi, H. Liu & P. Flook 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers [Review]. Annals of the Entomological Society of America

: 651-701.

Sutrisno, H., N. Azuma & S. Higashi 2006. Molecular phylogeny of the Indo-Australia Glyphodes and allied genera (Insecta: Lepidoptera: Crambidae) inferred from CO I, CO II and EF-1 alpha genes. Journal of Species Diversity 11: 57-69.

Sutrisno, H. 2008. Species status of yellow stem borer Scirpophaga incertulas (Lepidoptera: Pyralidae) based on CO I gene sequences. Treubia 36: 37-47.

Sutrisno, H. 2011. Molecular phylogeny of Indonesian Aganaine moths (Lepidoptera: Noctuidae) based on CO I gene. Treubia 38: 71-186.

Sutrisno, H. 2012. Molecular phylogeny of Indonesian armyworm Mythimna (Lepidoptera: Noctuidae). HAYATI (Journal of Biosciences) 19 (2): 60-65.

Swofford, D.L. 2001. PAUP*. Phylogenetic analysis using parsimony (* and Other Methods). Version 4.0b10 for 32-bit Microsoft Windows. Sinauer Associates, Sunderland, Massachusetts.

Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei & S. Kumar 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28: 2731-2739.

vanDorp, K. 2004. Molecular systematics of Lycaena F., 1807 (Lepidoptera: Lycaenidae) – Some

preliminary results. Procceding Netherland Entomological Society 15: 65-70.

Zhang, M., T.W. Cao, Y. Zhong, Y.P. & E.B. Guo 2011. Phylogeny of Limenitidinae butterflies (Lepidoptera: Nymphalidae) inferred from mitochondrial cytochrome oxidase I gene sequences. Agricultural Sciences in China 10 (4): 566-575.

Zhong, L., X. Xiao & Q. Zhang. 2000 Occurrence features of major insect pests of rice in Jiangxi Province in recent ten years. Jiangxi Plant Protection 23 (1): 8–9.

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