Karakterisasi Genetik Tempuyung (Sonchus arvensis L.) Berdasarkan Penanda Molekuler Sequence-Related Amplified Polymorphism

Abstract

Tempuyung (Sonchus arvensis L.) is known as an important medicinal plant used as a diuretics and
antihypertensives. This plant is widely distributed in Indonesia. Genetic diversity of tempuyung is important
information as a database for further research especially in medicinal plant standardization. The objective of this
study was to analyse genetic characterization of tempuyung based on SRAP (Sequence-related amplified
polymorphism) molecular markers. Thirteen samples were collected from 8 different locations and amplified using
5 primer SRAP combinations. Similarity matrix was calculated using Dice coefficient. Unweighted Pair Group
Method Using Arithmetic Mean (UPGMA) cluster analysis was performed to develop a dendrogram. The result
indicates that there was a genetic variation among tempuyung accessions and divided into 4 clusters with similarity
index of 0,7719. Citeureup and Turen3 accessions were the most closely similar with similarity index of 0,8936. In
conclusion, SRAP markers may serve as an efficient and effective tools to analyze the genetic diversity among
tempuyung accessions.
Keywords: genetic characterization, tempuyung (Sonchus arvensis L.), SRAP