PENGGUNAAN PACLOBUTRAZOLDAN ABA DAL AM PERBANYAKAN X NENAS SIMADU MELALUI KULTUR IN VITRO

Ragapadmi Purnamaningsih, Ika Mariska, Yati Supriati
10.14203/beritabiologi.v9i6.852
| Abstract views: 631 | PDF views: 894

Abstract

Pineapple (Ananas comosus L. Merr.), represents an important crop in Subang. Somaclonal variation is one of the problem to develop pineapple, especially Simadu variety. Probability to conduct Simadu progeny from the mother plant is very low (5%).Its caused by chimeric of the somatic cells that form meristem.In vitro culture is the alternative method to solve the problem by using the meristem cells from Simadu fruit as explant. Unfortunately, genetic diversity has been observed in many spesies during tissue culture.This phenomenon is usually termed somaclonal variation. Many studies on pineapple demonstrsted that some in vitro propagated materials differ from the source materials from which they are derived.To minimize genetic variability, the use of growth inhibitor such as paclobutazol and absisic acid hopefully would gave the important role in genetic stability. The aim of the research is to multiply Simadu pineapple by using tissue culture technic. In vitro shoot induce from crown of the Simadu fruit until get the sterile shoots. Combination of kinetin (0-5 ppm) with paclobutrazol ( 0-0.1 ppm) or ABA (0-1 ppm) was used in the multiplication stage. Result showed that there are no interaction between kinetin and paclobutrazol or ABA, but there is influence of the single factor. Kinetin increase leave number but decrease plant height and root number. Paclobutrazol increase shoot and leave number, but decrease plant height and root number. There is no influence of ABA to plant height, shoot and root number but decreased leaves number.

Keywords

Nenas, perbanyakan, kultur in vitro, paclobutrazol, ABA.

Full Text:

PDF

References

Anonim. 2002. Potensi dan Peluang Investasi Kabupaten Subang 2002. Bappeda Kabupaten Subang.

Anonim. 2003. The Biology and Ecology of Pineapple (Ananas comosus var Comosus) in Australia, http:// www.ogrv.gov.au/pdf/iv/pineapple.

Cote FX, JA Sandoval, Ph Marie and E Auborin. 1993. Variation in micropropagated bananas and plantains : literature survey. Fruits, 48, 15-22.

Davies PJ. 2005. The plant hormone concept : concentration, sensitivity and transport. In PJ Davies (Ed.). Plant Hormones. Phsiology, Biochemistry and Molecular Biology, 1-13. Kluwer Acad Pub .Netherlands.

Firoozabady E and N Gutterson. 2003. Cost effective in vitro propagation methods of pineapple. Plant Cell Rep. 21, 844-850.

Roostika I dan I Mariska. 2003. In vitro culture of pineapple through organogenesis and somatic embryogenesis : its utilization and prospect. Buletin AgroBio. 6(1), 34-60.

Firoozabady E and Y Moy. 2004. Regeneration of pineapple via somatic embryogenesis and organogenesis. In vitro Cell. Dev. Biol.-Plant. 40, 67-74.

George EF and PD Sherrington. 1984. Plant Propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories. Exegetics Livin. England.

Karp A. 1991. On the current understanding of somaclonal variation. Oxford Surveys of Plant Molecular and Cell Biologi 7, 1-58.

Kiss E, J Kiss, G Gyulai and LE Heszky. 1995. A novel method for rapid micropropagation of pineapple. Hort. Sci. 30(1), 127-129.

Methouachi JF, R Tadeo, S Zaragoza, E Rimo, Milko and M Toba. 1996. Effect of gibberellic acid and paclobutrazol on growth and carbohydrate accumulation in shoots and roots of citrus root stuck seedling. Hort Sci. 71(5), 747-754.

Oka S and T Nino. 1997. Long term storage of Pear (Pyrus spp). shoot culture in vitro by minimal growth method. Japan Agricultural Research Quarterly 31, 1-7.

Philips GC, JF Hubstenberger and EE Hansen. 1995. Adventitious shoot proliferation. In OL Gamborg and GC Philips (Eds.). Plant Cell, Tissue and Org Cult. Fundamental methods. Springer-Verlag Berlin Heidenberg, New York.

Pinhero RG and RA Fletcher. 1994. Paclobutrazol and ancymidol protect corn seedling from high and low temperature stresses. Plant Growth Reg. 15, 47-53.

Purnamaningsih R dan E Gati. 1997. Penyimpanan dan regenerasi pule pandak melalui kultur in vitro. Dalam Pros. Seminar Perhimpunan Bioteknologi Pertanian Indonesia. 12-14 Maret. Surabaya.

Ripaoraya S, R Marchant, B Power and MR Davey. 2003. Plant regeneration by somatic embryogenesis and organogenesis in commercial pineapple (Ananas comosus L.). In vitro Cell. Dev. Biol. - Plant 39, 450-454.

Salisbury FB and CW Ross. 1992. Plant Physiology. 4th edition. Wadsworth Publishing Co. Wattimena GA. 1988. Zat Pengatur Tumbuh Tanaman, 131-145. Pusat Antar Universitas, IPB. Bogor.

DOI: https://doi.org/10.14203/beritabiologi.v9i6.852

Refbacks

  • There are currently no refbacks.