PENAPISAN AKTIVITAS LIPOLITIK SEPULUH BIAKAN Rhizopus KOLEKSIUICC (UNIVERSITY OF INDONESIA CULTURE COLLECTION)

Inu Yuyun Lusini, Wibowo Mangunwardoyo 'ET, Indrawati Gandjar
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Abstract

Ten Rhizopus were screened for their extracellular lipolytic activity. All strains showed lipolytic activity with diffferent activities.R. microsporus var. rhizopodiformis (Cohn) Scipper & Stalpers UICC 520 (4.52 unit/ml) and R. microsporus var. oligosporus UICC 550 (2.58 unit/ml) showed a high lipolytic activity in screening medium containing 5% pepton and 1% glucose (b/v) without a lipid substrate after 24 hours of incubation at room temperature.

Keywords

Enzim ekstraselular, lipase, Rhizopus, University of Indonesia Culture Collection (UICC).

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References

Akhtar MW, AQ Mirza and MID Cnughtai. 1980. Lipase induction in Mucor hiemalis. Appll. and Environ. Microbiol. 40, 257-263.

Allonso FOM, EBL Oliveira, GM Deltamore-oritz and Pereira-Meirelles. 2005. Improvement of lipase production idt different stirrings speed & oxygen levels. Brazillian Journal of Chemistry Engineering 22(1), 9-18.

Davranov K and VB Khalameizer. 1997. Current state of the study of microbial lipases. Chemistry of Natural Compound 33, 113-126.

Ellibol M and D Ozer. 2002. Response surface analysis of lipase production by freely suspended Rhizopus arrhizus. Process Biochem. 38, 367-372.

Fukuda H, A Kondo and II Noda. 2001. Biodiesel fuel production by transesterification of oils. Journal of Bioscience & Bioengeneering 92, 405-416.

Gandjar I, IR Koentjoro, W Mangunwardoyo dan L Subagyo. 1992. Pedoman Praktikum Mikrobiologi Dasar. Jurusan Biologi FMIPA-UI. Depok.

Ha ma S, S Tamalampudi, T Fukumizu, K Miura, H Yamaji, A Kondo and H Fukuda. 2006. Lipase localization in Rhizopus oryzae cells immobilized within biomass support particles for use as whole-cell biocatalyst in biodiesel-fuel production. Journal of bioscience & bioengineering. 110(4), 328-333.

Kulkarni N. 2002. Studies on lipase enzyme from Pseudomonas fluorescens NS2W. PhD Thesis in Microbiology . University of Pune, India.ii+213 him.

Macris JB, E Kourentzi and DG Hatzinikolaou. 1996. Studies on localization and regulation of lipase production by Aspergillus niger. Procces Biochemistry 31(8), 807-812.

Madigan MT, JM Martinko and J Parker. 2000. Brock Biology of Microorganism. Prentice Hall. Upper Saddle-River. New Jersey.

Mangunwardoyo W, Santoso I, Fauzi R and MW Sugianto. 2004. Lipase activity of Rhizopus spp. in University of Indonesia Culture Collection. Proceedings of the Tenth International Congress For Culture Collection. Tsukuba, Japan, 10-15 Oktober 2004.

Mojovick L, S Siler-Marinkov, G Kuki and G Vunjak- Novakovic. 1993. Rhizopus arrhizus lipase-catalyzed interesterification of the midfraction of palm oil to a cocoa butter equivalent fat. Enzyme Microb. Technol. 15, 438-443.

Nahas E. 1988. Control of lipase production by Rhizopus oligosporus under variuos growth conditions. J. of General Microbiology 134, 227-233.

Pandey A, S Benjamin, CR Soccol, P Nigam, N Kriger and VT Soccol. 1999. The realms of lipases in biotechnology. Biotechnol. Appl. Biochem. 29, 119- 131.

Ramarethinam S, K Latha and N Rajalakshmi. 2002. Use of a fungal lipase for enhancement of aroma in black tea. Food Sci. Technol. Res. 8(4), 328-332.

Samad M, YA Samad, AB Salleh, CNA Razak, K Ampon, WMZW Yunus and M Basri. 1990. Lipase from a newly isolated thermophilic Rhizopus rhizopodiformis. World J. of Microbiology and Biotechnology 6, 390-394.

Schipper MAA and JA Stalper. 1984. A revision of the genus Rhizopus II. The Rhizopus microsporus-gmup. Studies in Mycology No.25, 20-34.

Sharma R, Y Chisti and UC Banerje. 2001. Review: Production, purificaton, characterization and application of lipases. Biotechnology Advances 19, 627-662.

Zhang LY and DZ Wei. 2003. Effective inducers for lipase production by Candida rugosa. Annals of Microbiology 53(4), 449-504.


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