KERAGAMAN GENETIK POPULASI IKAN NILA {Oreochromis niloticus) DALAM PROGRAM SELEKSI BERDASARKAN RAPD

Otong Zenal Arifin, Estu Nugroho, Rudhy Gustiano
10.14203/beritabiologi.v8i6.826
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Abstract

Objectives of the study was to discover genetic variability and genetic relationship of paternal half sib population of nile tilapia (Oreochromis niloticus) under Selection Program Scheme at Research Institute for Freshwater Aquaculture, in Bogor, West Java.Four populations from unrelated selected breeders were observed for genetic variability using RAPD. The analysis of amplification from each locus and fragments were used to estimate DNA polymorphisms, heterozygosity, fst and genetic distance.The range of heterozygosity of four examined populations was 0.1760-0.2168 with level polymorphic between 47.66% and 64.86%.The highest heterozygosity and polymorphic was on population 1 and the lowest one was on population 2. Fst test showed significance among the populations. The closest relationship was between population 1 and 4 (0.1978) and the furthest was population 2 and 3 (0.3289).

Keywords

Ikan nila, Oreochromis niloticus, RAPD, seleksi, kekerabatan, polimorfisme, heterozigositas, fst, jarak genetik.

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References

Abdallah HH, M Elnaldy, A Obeida and H Itriby. 2004. Genetic Diversity of Nile Tilapia Populations Revealed by Randomly Amplified Polymorphic DNA (RAPD). Aquaculture Research 35, 587-593.

Arifin OZ. 2006. Polimorfisme mtDNA Keturunan Pertama (Fl) dalam Seleksi Famili Ikan Nila {Oreochromis niloticus) di BBI Wanayasa, Jawa Barat. Tests. Pasca Sarjana, Institut Pertanian Bogor.

Asih S, E Nugroho dan Mulyasari. 2006. Penentuan Keragaman Genetik Ikan batak (Tor sorro) dari Sumatera Utara dengan Metode Analisis Random Amplified Polymorphism DNA (RAPD). Kumpulan Hasil Riset Tahun 2006. Balai Riset Perikanan Budidaya Air Tawar.

Falconer DS. 1989. Introduction to Quantitative Genetics. Longman Group Ltd, UK.

Harris AS, S Bieger, RW Doyle and SM Wright. 1991. DNA Fingerprinting of Tilapia Oreochromis niloticus and Its Application to Aquaculture Genetics. Aquaculture 92, 157-163.

Koh TL, G Khoo, LQ Fan and VPE Phang. 1999. Genetic Diversity Among Wild Forms and Cultivated Varieties of Discus (Symphysodon spp.) as Revealed by Random Amplified Polymorphic DNA (RAPD) Fingerprinting. Aquaculture 173,485-497.

Liu ZJ, P Li, BJ Argue and RA Dunham. 1999. Random Amplified Polymorphic DNA Makers: Usefulness for Gene Mapping and Analysis of Genetic Variation of Catfish. Aquaculture 174, 59-68.

Nei M and F Tajima. 1981. DNA Polymorphism Detectable by Restriction Endonucleases. Genetics 97, 146-163.

Pandin DS. 2000. Kemiripan Genetik Populasi Kelapa Dalam Mapanget Tenga, Bali, Palu dan Sawarna Berdasarkan Penanda RAPD. Tesis. Program Pasca Sarjana IPB. Bogor.

Rogers JS. 1972. Measures of Genetic Similarity and Genetic Distance. In: Studies in Genetics VII. University of Texas Publication No 7213, 145-154.

Roslim DI. 2001. Kemiripan Genetik Tiga Populasi Kelapa Dalam dari Tiga pulau dengan Penanda RAPD (Random Amplified Polymorphic DNA). Tesis. Program Pascasarjana IPB. Bogor.

Suryani SAMP, Sukoso dan Sugama. 2001. Hubungan Kekerabatan Tiga Spesies Ikan Kerapu Sunu (Plectropomus spp.) Atas Dasar Keragaman Genetik. Biosain. 1(3), 99-107.

Sudarto. 1990. Pengamatan Elektroforesis terhadap Ikan Tilapia (Oreochromis sp.). Bulletin Penelitian Perikanan Darat 9, 19-24.

Widiyati A. 2003. Keragaman Fenotipe dan Genotipe (Oreochromis niloticus) dari Danau Tempe (Sulawesi Selatan) dan Beberapa Sentra Produksi di Jawa Barat. Tesis. Pasca Sarjana, Institut Pertanian Bogor.

DOI: https://doi.org/10.14203/beritabiologi.v8i6.826

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