KERUSAKAN DINDING SEL Escherichia coli OLEH MINYAK ATSIRI TEMU KUNCI (Kaempferiapandurata)

Miksusanti Miksusanti, Betty Sri Laksmi Jennie, Bambang Ponco, Gatot Trimulyadi
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Abstract

Antibacterial activity of temu kunci (Kaempferiapandurata) essential oil against Escherichia coli Kl.l was analyzed. Activity of antibacterial essential oil was analyzed through it's ability to leak the Escherichia coli Kl.l cell wall and altering it.Minimum Inhibitory Concentration (MIC) of temu kunci essential oil is 0.11% (v/v). Further studies were conducted using the concentration of 1 MIC and 2 MIC.Leakage phenomena were monitored with atomic adsorption spectrometry (AAS), and ultraviolet spectrophotometry(UV).Alteration of cell wall was analyzed with scanning electron microscopy (SEM).The optical density values observed by UV spectrophotometer for protein and nucleic acid leakage were 0.3813-0.6573 at 280 nm and 0.2186-0.5603 at 260 nm.The result showed that K. pandurata essential oil could leak the inorganic ion Ca 17-53%, and K* 9-43% from the bacteria and alter the cell wall of the bacteria.

Keywords

Escherichia coli Kl.l, temu kunci, Kaempferia pandurata, kebocoran ion, perubahan dinding sel, protein, asam nukleat.

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