Ragapadmi Purnamaningsih, Misky Ashrina
| Abstract views: 408 | PDF views: 1638


Malaria is a global health problem that threatens 300-500 million people and kills more than one million people annually.Artemisinin, a sesquiterpen secondary plant metabolite extracted from Artemisia annua L., is a promising and potent antimalarial drug which has a remarkable activity against chloroquine resistant to Plasmodium falciparum. To counter the present low content(0.01-0.5%) of artemisinin in A. annua a limitation to commercial production of the drug and uneconomical chemical synthesis. A research was conducted to induce callus production by using Murashige-Skoog (MS) medium added with NAA (0, 0.5 and 1 mg/1) and BAP (0, 0.5 dan 1 mg/1) and also to produce artemisinin from the calli. Complete Randomized Design was used in the research. Callus cultures were induced from leaf explants of A. annua. The research reports succesful approach for production of artemisinin by callus cultures of A. Annua. Medium formulation of MS basal media added with plant growth regulators BAP 0.5 mg/1 and NAA 0.5 mg/1 give the best result for callus induction than others, with callus fresh weight 844,4 mg, artemisinin content 0.73%, dry weight 216.6 mg and total weight of artemisinin 1.58 mg.


Artemisia annua L, callus induction, artemisinin content.

Full Text:



Baldi A and VK Dixit. 2008. Enhanced artemisinin production by cell cultures of Artemisia annua. Current Trends in Biology and Pharmacy 2(2), 341-348.

Benjamin BD, AT Sipahimalani and MR Heble.1991. Tissue culture of Artemisia pallens: organogenesis, terpenoid production. Cell, Tissue and Organ Culture 21, 159-164.

Bhojwani SS and MK Razdan. 1996. Plant Tissue Culture: Theory and Practice, a Revised Edition. Department of Botany. Delhi. India.

Butler D. 1997. Time to put malarial control on the global agenda. Nature 386, 535-541.

Charles DJ, JE Simon, KV Wood and P Heinstein. 1990. Oermplasm variation in artemisinin content of Artemisia annua L. using an alternative method of artemisinin analysis from crude plant extract. Journal of Natural Products 53, 157-160.

Delabays N, X Simonnet, M Gaudin. 2001. The genetics of artemisinin content in Artemisia annua L. and the breeding of high yielding cultivars. Current Medical Chemistry 8, 1795-1801.

Ernawati A. 1992. Produksi senyawa-senyawa metabolit sekunder dengan kultur jaringan tanaman hal 169-208. Dalam: Bioteknologi Tanaman I. GA Wattimena, PAU Bioteknologi IPB. Bogor.

George EF and PD Sherrington. 1994. Plant Propagation by Tissue Culture. Exegetics Limited. England. Gunawan LV. 1987. Teknik Kultur Jaringan Tumbuhan, 167- 179. Laboratorium Kultur Jaringanlnstitut Pertanian Bogor.

Keng CL, N Singaram and BP Lim. 2010. Production of artemisinin from cell suspension culture of Artemisis annua L. Molecular Biology and Biotecnology 18(1), 139-141.

Nin SE, Morosi, S Schiff and ABennici 1996. Callus culture of Artemisia absinthium L. initiation, growth optimization and organogenesis. Plant Cell, Tissue and Organ Culture 45, 67-72.

Simon JE, D Charles, E Cebert, L Grant, J Janick and A Whipkey. 1990. Artemisia annua L.: Promising aromatic and medicinal, p. 522-526. In: J Janick.and JE. Simon (Eds.). Advances in New Crops. Timber press, Portland, OR.

WHO. 2004. More than 600 million people need effective malaria treatment to prevent unacceptably high death rates. Press release WHO/29, 22 April.

Zia M, RRehman and MF Chaudhary. 2007. Hormonal regulation for callogenesis and organogenesis of Artemisia absinthium L. African Journal of Biotechnology 6(16), 1874-1878.


  • There are currently no refbacks.