PENINGKATAN KADAR LOVASTATIN ANGKAK OLEH Monascuspurpureus KO-KULTUR DENGAN Endomycopsis burtontt

Danik D Asadayanti, B Sri Laksmi Jenie, Harsi D Kusu aningrum, Novik Nurhidayaf
10.14203/beritabiologi.v10i3.746
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Abstract

Lovastatin is a bioactive material of statin groups and has been used to reduce cholesterol through inhibiting HMG Co-A reductase enzyme activities. Three indigenous strains of Monascus purpureus and three mutans were used in this study produced lovastatin at the range of 0,1 - 1,42%. The objectives of this study were to increase lovastatin productions by co-cultured with several concentrations of Endomycopsis burtonii. M. purpureus (10 cfu/ml) was co-cultured with various concentrations of E. burtonii (10M0 ) cfu/ral at three different feeding times (day 2, 4, and 6). Feeding times and concentrations of E. burtonii significantly increased production of lovastatin by four strains of M. purpureus (JMBA5K, AID, JMBA and TOS). The highest production of lovastatin was achieved by M. purpureus TOS co-cultured by E. burtonii at 10 cfu/ml added at day 6. Expression of the genes that responsible for lovastatin production were analyzed by PCR and RT-PCR method. The phenotypic character of M. purpureus TOS with high lovastatin production was conformed by the high intensity of its gene expression.

Keywords

Lovastatin, angkak, Monascus purpureus, Endomycopsis burtonii

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References

Alberts AW, J Chen, G Kuron, V Hunt, J Huff and C Hoffman. 1980. Mevinolin: A. highly potent competitive inhibitor of hydroxymethyl glutaryl coenzyme A reductase and cholesterol lowering agent. Proceedings of the National Academy of Sciences of the United States of America 77(7), 3957-3961.

Banerjee R, G Mukherjee and KC Patra. 2005. Microbial transformation of tannin-rich substrate to gallic acid through co-culture method. Bioresource Technology 96(8), 949-953.

Chiu CH, NiKH Guu, YK TM Pan. 2006. Production of red mold rice using a modified Nagata type Koji marker. Applied Microbiology and Biotechnology 73(2), 297-304.

Chomenzynski P and N Sacchi. 1987. Single step methods of RNA isolation by acid guanidium thiocyanatephenol- chloroform extraction. Analytical Biochemistry, 162,156-159.

Danuri H. 2008. Optimizing angkak pigment and lovastatin production by Monascus purpureus. Hayati - Journal of Bioscience June, 61-66.

Handayani WR. 2006. Penurunan ekspresi gen pho85 sel apoptosis Saccharomyces cerevisiae oleh ekstrak air daun ciplukan 33NHR dan Geobacillus sp. 22a. Bogor. Thesis, IPB, Bogor.

Hajjaj H, P Niedberger and P Duboc. 2001. Lovastatin biosynthesis by Aspergillus terreus in chemically defined medium. Applied and Environmental Microbiology 67(6), 2596-2604.

Kohama Y, S Matsumoto, T Mimura, N Tanabe, A Inada and T Nakanishi, T. (1987). Isolation and identification of hypotensive principles in red-mold rice. In: Chemical and pharmaceutical Bulletin 35(6), 2484-2489.

Lee CL, JJ Wong, SL Kuo and TM Pan. 2006. Monascus fermentation of dioscorea for increasing the production of cholesterol-lowering agents-monacolin K and antiinflammation agent-monascin. Applied Microbiology and Biotechnology 72(6), 1254-1262.

Lim HS, SK Yoo, CS Shin and YM Hyun. 2000. Monascus red pigment overproduction by co-culture with recombinant Saccharomyces cerevisiae secreting glucoamylase. The Journal of Microbiology, March, 48-51.

Miyake T, A Mori, T Kii , T Okuno, Y Usui and S Fumihiro. 2005. Light effect on cell development and secondary metabolism in Monascus. Journal of Industrial Microbiology and Biotechnology 32(3), 103-108.

Miyake T, S Ohno and S Sakai. 1984. Process for the production of Monascus pigment. United States Patern 4442, 209.

Murray, K Robert , K Daryl , Granner, A Petter , Mayes, W Victor and Rodwell. 2003. Harper's Illustrated Biochemistry, 26. New York: Me Graw-Hill Companies.

Panda BP, S Javed and M Ali. 2010. Optimization of fermentation parameter for higher lovastatin production in red mold rice through co-culture of Monascus purpureus and Monascus ruber. Food Bioprocess Technology 3: 373-378.

Pandey A, P Selvakumar, CR Socool and P Nigam. 1999. Solid-state fermentation for production of industrial enzymes. Current Science 77(1), 149-162.

Sambrook J. 1989. Molecular Cloning - A Laboratory manual. Ed ke-1. Cold Spring Harbor Laboratory Press. New York.

Shin CS, HJ Kim, MJ Kim and JY Ju. 2005. Morphological change and enhanced pigmen production of Monascus when co-cultured with Saccharomyces cereviseae or Aspergillus oryzae. Biotechnology and Bioengineermg 59, 576-581.

Su YC, JJ Wang, TT Lin and TM Pan. 2003. Production of secondary metabolites, y-amino butyric acid and monacolin K by Monascus. Journal of Industrial Microbiology and Biotechnology 30(1), 41-46.

Termudo MF, R Kleerebezem and M Loosdrecht. 2007. Influence of the pH on (open) mixed culture fermentation of glucose: A chemostat study. Biotechnology and Bioengineering 98(1), 69-79.

Wilson K and J Walker. 2000. Principles and Techniques of Practical Biochemistry. London. Cambridge University.

DOI: https://doi.org/10.14203/beritabiologi.v10i3.746

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