RESPON PADI TRANSGENIK CV. NIPPONBARE GENERASI T1 YANG MENGANDUNG GEN Oryza sativa DEHYDRATION-RESPONSE ELEMENT BINDING 1A (OsDREB1A) TERHADAP CEKAMAN SALINITAS

Tri Joko Santoso, Aniversari Apriana, Atmitri Sisharmini, Kurniawan Rudi Trijatmiko
| Abstract views: 203 | PDF views: 516

Abstract

Salinity is one of the abiotic constraints in the cultivation of rice crop. One of the reasons agricultural land becomes saline is due to the intrusion of seawater into the mainland as a result of global climate change. Dehydration-responsive element binding (DREB) gene is a plant -specific transcription factor gene that have important role in regulating plant responses to abiotic stresses, including high salinity. Transgenic rice plants cv. Nipponbare carrying OsDREB1A gene have been generated. However, study of the response of putative transgenic plants to salinity has not been done. The research objective is to study the response of T1 generation Nipponbare-OsDREB1A transgenic rice plants to salinity stress. The result showed that the response of putative transgenic rice Nipponbare-OsDREB1A to salinity stress 25 mM and 150 mM NaCl indicated a level of tolerance varies from highly sensitive to highly tolerance. These variations were possibly occurred because of the segregation state of the T1 generation transgenic rice. Based on damage symptom scoring and PCR analysis provided information that transgenic rice plant cv. Nipponbare-OsDREB1A which showed positive PCR had a very high tolerance to salinity stress 150 mM compared with non-transgenic rice cv. Nipponbare.

Keywords

Rice (Oryza sativa L.), transgenic plant, OsDREB1A gene, salinity, PCR

Full Text:

PDF

References

Balai Penelitian Rawa(Balittra). 2005. Laporan Tahunan Penelitian Pertanian Lahan Rawa Tahun 2004. Trip Alihamsyah dan Izzuddin Noor (Penyunting). Balai Penelitian Pertanian Lahan Rawa. Banjarbaru

Doyle JJ and JL Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12, 13-15

Dubouzet JG, Y Sakuma, Y Ito, M Kasuga, EG Dubouzet, S Miura, M Seki, K Shinozaki and K Yamaguchi- Shinozaki. 2003. OsDREB genes in rice, Oryza sativa encode transcription activators that function in drought, high-salt and cold-responsive gene expression. Plant Journal 33, 751-763.

Gregorio. 1997. Screening rice for salinity tolerance. IRRI Discussion 22, 3-13.

Haake V, DCook, JLRiechmann, MF Thomashow and JZ Zhang. 2002. Transcription faktor DREB is a regulator of drought adaptation in Arabidopsis. Plant Physiology 130, 639–648.

Harjadi SS dan S Yahya. 1988. Fisiologi Stress Lingkungan. PAU Bioteknologi-Institut Pertanian Bogor.

Hu H, M Dai, J Yao, B Xiao, X Li, Q Zhang and L Xiong. 2008. Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proceeding National Academy Science USA 103, 12987-12992.

Kasuga M, Q Liu, Miura S, K Yamaguchi-Shinozaki and Shinozaki K. 1999. Improving plant drought, salt and freezing tolerance by gene transfer of a single stressinducible transcription factor. National Biotechnology 17, 287-291.

Las I, E Surmaini dan A Ruskandar. 2008. Antisipasi perubahan iklim: inovasi teknologi dan arah penelitian padi di Indonesia. Prosiding Seminar Nasional Padi 2008: Inovasi Teknologi Padi Mengantisipasi Perubahan Iklim Global Mendukung Ketahanan Pangan. Balai Besar Penelitian dan Pengembangan Tanaman Padi, Sukamandi.

Oh SJ, IS Sang, SK Youn, JJ Hyun, YK Soo, K Minjeong, KK Yeon, HN Baek and KK Ju. 2005. Arabidopsis CBF3/ DREB1A and ABF3 in Transgenic Rice Increased Tolerance to Abiotic Stress Stunting Growth. Plant Physiology. 138, 341-351.

Santoso TJ, A Apriana, A Sisharmini, Tasliah, S Moeljopawiro dan IH Soemantri. 2009. Perakitan Padi Transgenik Toleran Kekeringan dan Tahan Blas Yang Dapat Menekan 20% Kehilangan Hasil dengan Produktivitas 7-8,5 ton/ha. Laporan Tahunan Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian. Bogor.

Sembiring H, A Gani and T Iskandar. 2008. Implications of salinity research in Aceh for indonesian rice growing, In: F Agus and G Tinning (Eds.). Proceedings on International Workshop on Post Tsunami Soil Management. Soil Research Institute-Ministry of Agriculture Indonesian-Soil Research Institute.

Soertini S. 2003. Aplikasi mutasi induksi dan variasi somaklonal dalam pemuliaan tanaman. Jurnal Penelitian dan Pengembangan Pertanian 22(2), 70-78.

Xiong YW and SZ Fei. 2006. Functional and phylogenetic analysis of a DREB/CBF-like gene in Perennial ryegrass (Lolium perenne L.). Planta 224, 878-888.

Yunus M, D Suardi, KR Trijatmiko, Tasliah dan A Dadang. 2007. Identifikasi gen toleran kekeringan pada padi dengan menggunakan analisis DNA microarrays. Laporan Akhir Penelitian. Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian. Bogor.


Refbacks

  • There are currently no refbacks.