PERBANYAKAN IN VITRO KELADI TIKUS (Typhonium flagelliforme (Lodd) BI.), TANAMANA YANG BERPOTENSI SEBAGAI OBAT KANKER

Maria Imelda
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Abstract

Keladi tikus (Typhonium flagelliforme (Lodd) BI.) of the Araceae generally grows wild in the open or in slightly shaded areas. The active compounds of the plant have not yet been known, however its potential for cancer medication has been reported by several media. Since the plant is being hevaly removed in their habitat, research for adoption of in vitro technique for production of their planting materilals is needed. Lateral shoots of a 2-cm tuber, were sterilized with 30% sodium hypochlorite for 15 minutes. After several rinses with sterile distilled water, the shoots were cultured on a soild Murashige & Skoog (MS) medium supplemented with BAP (0,25;0,5;0,75;1,0 mg/l) in combination with IBA (0,25;0,5;0,75;1,0 mg/l). All cultures were incubated at 26 C under fluorestcent lights of 16 hours/day. Rooting of shoots was induced on MS medium without growth regulators and the plantets were established in a potting medium of sand,compost and soil (1:1:1). The result showed that the highest rate of shoot proliferation and growth was on MS medium supplemented with 0,5 mg/l 1 BAP and 0.25 mg/l IBA. Using this medium,  a sixteen-fold increase in shoot multiplication can be achieved with survival rate of 95%.

Keywords

Keladi tikus (Typhonium flagelliforme (Lodd) BI.), in-vitro progation,growth regulator, BAP,IBA

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References

Backer CA and Bakhuisen van den Brink Jr B. 1968. Flora of Java Vol. III Wolters Noordhoff N.V.-Groningen- The Netherlands. Hlm 125.

George EF and Sherrington PD. 1984. Plant Propagation by Tissue Culture, Handbook and Directory of Commercial Laboratories. Eastern Press, Reading, UK. Hlm 284-330.

Hartmann HT, Kester DE and Davies Jr FT. 1990. Palnt Propagation, Principles and Practices, fifth edition. Prentice- Hall Internasional, Inc., New Jersey. Hlm 459-484.

Murashige T and Skoog F. 1962. A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiol. Plant. 15: 473-497.

Teo CKH and Chíng BI. 1996. Cancer, yet They Live. Eramaps Sdn. Bhd, Malaysia. Hlm. 53-70.

Ziv M. 1994. In vitro Acclimatization. Dalam: Automation and Enviromental Control in Plant Tissue Culture. Aitken-Chistie j, Kozai T and Smith MAL (eds.). Kluwer Academic Publishers Dordrecht. Hlm 493-516.


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