SELEKSI BERBANTUKAN MARKA MOLEKULER UNTUK PEMBENTUKAN CALON VARIETAS PADI TURUNAN ESENSIAL SITU PATENGGANG

Siti Yuriyah, Dwinita Wikan Utami, Siti Nurani, Anggiani Nasution, Puji Lestari, Ahmad Dadang, Suwarno Suwarno
| Abstract views: 85 | PDF views: 135

Abstract

The utilization of molecular marker technology for Blast (Pyricularia grisea) resistance in rice breeding could accelerate and improve the precision of selection. This study aimed to identify and to select the BC3F2 progenies from Situ Patenggang and the opted Blast Monogenic Lines based on their resistance to blast disease in green house and field using molecular characterization. A total of 200-300 BC3F2 population strains resulted in 4 crosses between Situ Patenggang varieties and Monogenic Varieties IRBLta2-Re, IRBLkp-k60, IRBLi-F5 and IRBLa-A were used as total genetic material in the study. Blast resistance assay was initially carried out in a greenhouse and further selection was conducted in the endemic blast area, Sukabumi. The selected Molecular marker  was STS (Sequence Taq Sites) marker for foreground selection and 384-SNPs (Single Nucleotide Polymorphism) chip for background selection. The number of lines from each cross was selected by greenhouse assay i.e: 25 lines derived from Situ Patenggang/IRBLta and Situ Patenggang/IRBLkp, 21 plants for crossing Situ Patenggang/IRBLi, and 22 plants for Situ Patenggang/IRBLa-A. The results of field  experiment revealed that blast resistance response of the selected lines was varied from  0 to 5. Lines of foreground were successfully selected using STS markers specified for Pii, Pita, Pikp and Pia genes. As for the background selection by SNPs markers, some lines carried the recurrent parent genetic background, Situ Patenggang. Overall, 20 resistance lines that harbored the target genes and Situ Patenggang background were obtained. Further observation was entailed to these twenty selected lines in order to attain promising lines candidate for blast resistance.

 

 

 

 

Keywords

Foregound-background selection, Rice blast disease, STS & SNP markers.

Full Text:

PDF

References

Agrama, H.A., Eizenga, G.C. and Yan, W., 2007. Association mapping of yield and its components in rice cultivars. Molecular Breeding, 19, pp. 341–356.

Lestari, A., Widyastuti, U. dan Enggarini, W., 2016. Uji Virulensi 100 Isolat Cendawan Blas (Pyricularia oryzae Cavara) terhadap Satu Set Varietas Padi Diferensial Indonesia. Jurnal Agrotek Indonesia, 1(1), pp. 37–46.

Amir, M., Nasution, A. dan Santoso., 2000. Inventarisasi Ras P. grisea di Daerah Sukabumi Jawa Barat Musim Tanam 1995-1998. pp. 148-151. Dalam: Soedarmono, Arwiyanto, T., Donowidjojo, S.,Djatmiko, H.A., Utami, D.S., Prihatiningsih, N., Pramono, A. dan Mugiastuti, E. ed. Prosiding Kongres Nasional XV. PFI. Purwokerto.

Ashkani, S., Rafii, M.Y., Shabanimofrad, M., Ghasemzadeh, A., Ravanfar S. and Latif, M.A., 2015. Molecular progress on the mapping and cloning of functional genes for blast disease in rice (Oryza sativa L.): current status and future considerations. Critical Reviews in Biotechnology, 14 , pp.1–15.

Baker, B., Zambryski, P., Staskawicz, B. and Dinesh-Kumar, S.P., 1997. Signalling in plant-microbe interactions. Science, 276 , pp. 726–733.

Bradbury, Peter, J., Zhiwu, Z., Dallas, E.K., Terry, M., Casteven, Yoges, R. and Buckler, E.S., 2007. Tassel: Software for Association Mapping of Complex Traits in Diverse Samples. Bioinformatics, (23), p.19.

Brondani, C., Brondani, R.P.V., Garrido, L.R. and Ferrira, M.E., 2000. Develpoment of microsatelite for the genetic analysis of Magnaporthe grisea. Gentics and Molecular Biology, 23(4), pp.753–762.

Direktorat Jendral Tanaman Pangan. 2013. Perkembangan Serangan Penyakit Blas Pyricularia Grisea. http;//tanamanpangan.pertanian.go.id/ditlintp/berita-150-perkembangan-serangan-penyakit-blas-pyricularia-grisea-sacc. (dikases 17 April 2016).

Doyle, J.J. and Doyle, J.L., 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19(1), pp. 11–15.

Dunning, M.J., Barbosa-Morais, N.L., Lynch, A.G., Tavaré, S. and Ritchie, M.E., 2008. Statistical issues in the analysis of Illumina data. BMC bioinformatics, 9(1), p.85.

Fathurrahman, I., Lutfi, M., Ciptadi, A.Y. dan Aris, P., 2010. Penyakit pada Tanaman Padi. Departemen Proteksi Tanaman, Fakultas Pertanian Institut Pertanian Bogor, Bogor.

Feltus, A.F., Wan, J., Schulze, S.R., Estill, J.C., Jiang, N. and Paterson, A.H., 2004. An SNP resource for rice genetics and breeding based on subspecies Indica and Japonica. Genome Research, 14, pp. 1812–1819.

Hayashi, N. and Fukuta, Y., 2009. Development and Characterization of Blast Resistance Using Differential Varieties in Rice, Japan International Research Center for Agricultural Sciences, Japan. p.11.

Hospital, F., 2009. Challenges for effective marker-assisted selection in plants. Genet. 136:303-310. doi:10.1007/s10709-008-9307-1

Illumina Product Guide., 2009. SNP Genotyping and Copy Number Analysis. Illumina Inc. San Diego, Amerika Serikat.

Kementerian Pertanian RI., 2012. Pedoman Teknis Pelaksanaan Indikasi Geografis Tahun 2012. Direktorat Pengembangan Usaha dan Investasi, Direktorat Jenderal Pengolahan dan Pemasaran Hasil Pertanian, Kementerian Pertanian RI.

Koide, Y., Kobayashi, N., Xu, D. and Fukuta, Y., 2009. Review: Resistances gene and selection DNA markers for blast disease in rice (Oryza sativa L.). Jarq Japan agricultural research quarterly, 43(4), pp. 225–280.

Lukman, R., Afifuddin, A. dan Hoerussalam., 2013. Pemanfaatan teknologi molecular breeding dalam pemuliaan ketahanan tanaman terhadap hama dan penyakit. Agroteknologi, 3(2), pp. 101–108.

Noguchi, M.T., Yasuda, N. and Fujita, Y., 2007. Fitness character un parasexual recombinants of the blast fungus Pyricularia oryzae. Jarq Japan agricultural research quarterly. 41 (2). p. 123–131.

Roychowdhury, M., Jia, Y. and Cartwright, R.D., 2012. Structure, functionand co-evolution of rice blast resistance genes. Akta Agronimica Sinica, 38(3), pp. 381–393.

Sadegh, A., Rafii, M.Y., Shabanimofrad, M., Miah, G., Sahebi, M., Azizi, P., Tanweer, F.A Akhtar, M.S. and Nasehi, A., 2015. Frontiers in Plant Science, 2015; 6, p. 886.Published online 2015 Nov 16. doi: 10.3389/fpls.2015.00886

Santoso, A.N., Utami, D.W., Hanarida, I., Ambarwati, A.D., Mulyopawiro, S. dan Tharreau, D., 2007. Variasi genetik dan spectrum virulensi pathogen blas pada padi asal Jawa Barat dan Sumatera. Jurnal Penelitian Pertanian Tanaman Pangan, 26(3), pp. 150–155.

Santoso, dan Nasution A., 2009. Pengendalian penyakit blas dan penyakit cendawan lainnya. Dalam: Inovasi Teknologi Produksi Padi. Buku 2. Balai Besar Penelitian Tanaman Padi. Badan Penelitian dan Pengembangan Pertanian. pp. 531–563.

Sambrook, J. and Russel, D.W., 2001. Molecular Cloning: A Laboratory Manual. 3rded. CJHL. New York. pp. XXVIII+18-136+A.14.1+R22+1.44.

Sallaud, C., Loriux, M., Tharreau, D., Berruyer, R., Svestatasrani, P., Garsrmeur, O., Guequiere, A. and otteghem, J.L., 2003. Identification of five new blast resistance genes in highly blast resistance variety IR64 using a QTL mapping Strategy. Theoretical and Applied Genetics, 106, pp.794–803.

Sharma, T., Rai, A., Gupta, S., Vijayan, J., Devanna, B. and Ray, S., 2012. Rice blast management through host-plant resistance: retrospect and prospects. Agricultural Research, 1, pp. 37–52. 10.1007/s40003-011-0003-5

Scardaci, S.C., Webster, R.K., Greer, C.A., Hill, J.E., William J.F., Mutters, R.G., Brandon, D.M., McKenzie, K.S. and Oster, J.J., 1997. Rice blast: a new disease in California. Agronomy Fact Sheet Series. 1997-2. Departement of Agronomy and Range Science, University of California, Davis.

Sri Indrayani, Anggiani Nasution dan Enung S. Mulyaningsih., 2013. Analisis ketahanan padi gogo dan padi sawah (Oryza sativa L.) terhadap empat ras penyakit blas (Pyricularia grisea Sacc), Jurnal Agricola, 3(1), pp. 53–62.

Suwarno, E., Lubis. and Soenarjo, E., 2001. “Breeding of Upland Rice for resistance to Blast in Indonesia.” In Kardin, M.K. et al. (Ed.), Upland Rice Research in Indonesia: Current Status and Futures Direction. pp. 7–14. CRIFC-IRRI Collaborative Research. Central Research Institute for Food Crops, Bogor. Indonesia.

Suwarno, Lubis, E., Hairmansis, A. dan Nasution, A., 2008. Pembentukan paket 20 varietas padi gogo untuk pengendalian penyakit blas. Simposium V tanaman pangan. Prosiding Simposium V Tanaman Pangan. Inovasi Teknologi Tanaman Pangan. Buku 2 Penelitian dan Pengembangan Padi. Bogor. Puspen PTP.

Utami, D.W., Ambarwati, A.D., Apriana, A., Sisharmini, A., Hanarida, I., Tharreau, D. and Santoso., 2007. Spektrum ketahanan galur haploid ganda turunan IR64 dan Oryza rufipogon yang mengandung QTL ketahanan terhadap penyakit blas (Pir). Agrobiogen, 3(1), pp. 1–8.

Utami, D.W., Rosdianti, I., Dewi, I.S., Ambarwati, D., Sisharmini, A., Apriana, A., Yuriyah, S., Ridwan, I. and Somantri, I.H., 2016. Utilization of 384 SNP genotyping technology for seed purity testing of new Indonesian rice varieties Inpari Blas and Inpari HDB. SABRAO. Journal of Breeding and Genetics, 48 (4), pp. 416–424.


Refbacks

  • There are currently no refbacks.