Dwinita Wikan Utami, I Rosdianti, S Yuriyah, AD Ambarwati, I Hanarida, Suwarno Suwarno, Miftahudin Miftahudin
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Program on the ability of agriculture to provide rice as a staple food tends to decrease with the reduced land area used for rice cultivation. improvement rice production should be directed to use  marginal land  as dry and acid land, where poisoning aluminium was seen as a constrain factor. Development of Aluminium (Al) tolerant variety is needed to increase rice production. This technology was environmentally friendly, cheaper and also easier for farmers adopted. The rice lines with broad genetics background which tolerant to Al toxicity has been produced by breeding program. The diversity of Indonesian local rice germplasm is a basic foundation on development of Al toxicity tolerant rice varieties. The purpose of this research was to analyze the genotype variety using 384 SNPs markers on selected Al tolerant rice lines based on bioinformatics approach. Based on the 384 SNPs markers, the total 26 selected  genotypes were clustered into 5 groups. Each group was having varied Relative Root Length (RRL), which was an Al tolerant parameter of phenotype. These groups were indicated for having different genes or QTL that were related to the Al tolerant character. The association analysis obtained 9 significant SNPs markers that can be applied as the molecular markers for assisting selection in breeding  program.


Gen / QTL forAluminium tolerant; molecular markers; upland rice

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Baligar VC, NK Fageria and ZL He. 2001. Nutrien use efficien-cy in plants. Communications in Soil Science and Plant Analysis 32, 921-950.

Delhaize E and PR Ryan. 1995. Aluminum toxicity and toler-ance in plants. Plant Physiology 107, 315-321.

Harter RD. 2002. Acid soil of the tropic. An Echo Tech Note. [].(Diunduh tanggal 29 November 2016.)

Khatiwada SP, D Senadhira, AL Carpena, RS Zeigler and PG Fernandez. 1996. Variability and genetics of toler-ance for aluminum toxicity in rice (Oryza sativa L.). Theoretical and Applied Genetics 93, 738-744.

Lynch JP and SB St. Clair. 2004. Mineral stress: The missing link in understanding how global climate change will affect plants in real world soils. Field Crops Research 90, 101-115.

Ma JF, R Shen, Z Zhao, M Wissuwa, Y Takeuchi, T Ebitani and M Yano. 2002. Response of rice to Al stress and identification of quantitative trait loci for Al tolerance. Plant and Cell Physiology 43, 652-659.

Ma JF, S Nagao, CF Huang and M Nishimura. 2005. Isolation and characterization of a rice mutant hypersensitive to Al. Plant and Cell Physiology 46(7), 1054- 1061.

Maschner H. 1995. Mineral Nutrition of Higher Plants, 889. Academic, New York.

Mossor-Pietraszewska T. 2001. Effect of aluminium on plant growth and metabolism. Acta Biochimika Polonica 48(3), 673-683.

Miftahudin, GJ Scholes and JP Gustafson. 2002. AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereal L.). Theoretical and Applied Genetics 104(4), 626-631.

Mulyani A, A Rachman dan A Dairah. 2009. Penyebaran Lahan Masam, Potensi, dan Ketersediannya untuk Pengembangan Pertanian, 23. Balai Penelitian Tanah. Bogor.

Nguyen BD. 2001a. Molecular mapping of aluminum tolerance in rice. Texas Tech University. [Disertasi].

Nguyen VT, MD Burow, HT Nguyen, BT Le, TD Le and AH Paterson.2 001b. Molecular mapping of genes con-ferring aluminum tolerance in rice (Oryza sativa L.). Theoretical and Applied Genetics 102, 1002-1010.

Nguyen VT, BD Nguyen, S Sarkarung, C Martinez, AH Pater-son and HT Nguyen. 2002. Mapping of genes con-trolling aluminum tolerance in rice: Comparison of different genetics background. Molecular Genetics and Genomics 267, 772-780.

Nguyen BD, DS Brar, BC Bui, TV Nguyen, LN Pham andHT Nguyen. 2003. Identification and mapping of the QTL for aluminum tolerance introgressed from the new source, Oryza rufipogon Griff.into Indica rice (Oryza sativa L.). Theoretical and Applied Genetics 106, 583-593.

Roesmarkam S, Subandi dan E Muchlis. 1992. Penyaringan material koleksi shorgum di tanah masam PMK. Jurnal Penelitian Pertanian 11, 366-370.

Rongwen J, MS Akkaya and AA Bhagwat. 1995. The use of microsatellite DNA markers for soybean genotype identification. Theoretical and Applied Genetics 90, 43-48.

Sanchez PA. 1992. Sifat dan Pengelolaan Tanah Tropika. 397. Institut Teknologi Bandung.

Wu P, B Zhao, J Yan. A Luo, Y Wu and D Senadhira. 1997. Genetics control of seedling tolerance to aluminum toxicity in rice. Eupyhtica 97, 289-293.

Wu P, CY Liao, B Hu, KK Yi, WZ Jin, JJ Ni and C He. 2000. QTLs and epistasis for aluminum tolerance in rice (Oryza sativa L.) at different seedling stages. Theoreti-cal and Applied Genetics 100, 1295-1303.


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