PENINGKATAN PERTUMBUHAN PADI VAR CIHERANG SETELAH DIINOKULASI DENGAN Azospirillum MUTAN MULTIFUNGSI PENAMBAT N2, PELARUT P DAN PENGHASIL FITOHORMON INDOLE ACETIC ACID (IAA).

Eny Ida Riyanti, Edy Listanto
| Abstract views: 856 | PDF views: 1054

Abstract

Modern agriculture rellies on the application of fertilizer to enhanched plant growth. Excessive application of chemical fertilizers in the long term will decrease soil fertility, therefore the use of biological fertilizers is expected to reduce the negative impact. The aim of this study was to determine the effect of multi-functional Azospirillum for N2-fixation, phosphate solubilizer  and producing phytohormone Indole Acetic Acid (IAA) on the growth of rice plants Ciherang in the pot experiment conducted in the BB Biogen’s greenhouse. Two treatments were tested: types of inoculation (not inoculated, were inoculated with the wildtype AjB 6.4.1.2 and inoculated with mutant isolates AJM 3.7.1.14), and 4 levels of fertilizer application (not fertilized, a quarter dose of paddy fertilizer recommendations, a half dose of fertilizer rice in the rice fields, and the appropriate dose of fertilizer in paddy fields). Azospirillum used is wildtype isolate AjB 6.4.1.2 and mutant isolate AJM 3.7.1.14 isolated and and mutated in BB Biogen. Ciherang rice seed inoculated with Azospirillum on the cell density of 106 cells / ml at the different seedlings tub. After the age of 14 days after planting, the seedlings were transferred into pots with three plants per pot. Parameters measured were: plant height, number of tillers, number of panicles per clump, fresh weight and dry panicles per clump, weight of 100 grains, and the content of N and P stover. Results show that inoculantion, affect significantly on the number of panicles per hill, grain weight per panicle and the dry weight of grains per panicle.

 

Keywords

Azospirillum, phytohormone, phosphat solubilizer, rice

Full Text:

PDF

References

Balitbangtan, 2016. Teknologi Jajar Legowo Super untuk Mendongkrak Produktivitas Padi. http://www.litbang. pertanian.go.id/berita/one/2574/. (Diunduh 12 Oktober 2016.

Bashan Y and G Holguín. 1998. Proposal for the Division of Plant Growth Promoting Rhizobacteria into Two Classifications: Biocontrol-PGPB (Plant Growth Promoting Bacteria) and PGPB. Soil Biology & Biochemistry 30, 1225-1228.

Bashan Y, G Holguin and L de-Bashan. 2004. Azospirillum - Plant Relationships: Physiological, Molecular, Agricultural, and Environmental Advances (1997-2003). Canadian Journal of Microbiology 50,521-577.

Döbereiner J and F Pedroza. 1987. Nitrogen-Fixing Bacteria in Non Leguminous Crop Plants,155. Science Technology, Madison, WI.

Hadiarto T, Ma’sumah dan EI Riyanti. 2012. Pembentukan Populasi Mutan Azospirillumdengan Menggunakan Transposon untuk Sifat Superior terhadap Pelarutan P. Jurnal AgroBiogen 8(2), 62-68.

Kapulnik Y, S Sarig, I Nur, Y Okon, J Kigel and Y Henis. 1981. Yield Increases in Summer Cereal Crops of Israeli Fields Inoculated with Azospirillum. Experimental Agriculture 17, 179-187.

Kapulnik Y, S Sarig, I Nur and Y Okon. 1983. Effect of Azospirillum Inoculation on Yield of Field-Grown Wheat. Canadian Journal of Microbiology 29,895-899.

Kapulnik Y, Y Okon and Y Hems. 1987. Yield Response of Spring Wheat Cultivars (Triticum aestivum and T. turgidum) to Inoculation with Azospirillum brasilense Under Field Conditions. Biologyand Fertility of Soils 4, 27-35.

Lestari P, DN Susilowati dan EI Riyanti. 2007. Pengaruh Hormon Asam Indol Asetat yang Dihasilkan Azospirillum sp. terhadap Perkembangan Akar Padi. Jurnal AgroBiogen 3(2), 66-72.

Rao VR, DN Nayak, PBBN Charyulu and TK Adhay. 1983. Yield Responses of Rice to Root Inoculation with Azospirillum. Journal of Agricultural Science 100,689-691.

Riyanti, EI. 2011. Rekayasa Genetik Azospirillum Unggul untuk Menurunkan Penggunaan Pupuk Nitrogen Sebesar 30% dan penggunaan Pupuk Fosfat Sebesar 15% dari Standard Pemupukan untuk Padi Sawah, 1-53. Badan Penelitian dan Pengembangan Pertanian, Jakarta.

Riyanti, EI. 2010. Rekayasa Genetik Azospirillum Unggul untuk Menurunkan Penggunaan Pupuk Nitrogen Sebesar 30% dan penggunaan Pupuk Fosfat Sebesar 15% dari Standard Pemupukan untuk Padi Sawah, 1-35. Badan Penelitian dan Pengembangan Pertanian, Jakarta.

Riyanti EI, T Hadiarto and DN Susilowati. 2012. Multi Functional Mutants of Azospirillum sp with Enhanced Capability of Solubilizing P, Fixing N2 and Producing Phytohormone Indole Acetic Acid. Indonesian Journal of Agricultural Science 13(1), 12-17.

Tien TM, H Gaskins and DH Hubbell. 1979. Plant Growth Substances Produced by Azospirillum brasilense and their Effect on the Growth of Pearl Millet (Pennisetum americanum L.). Applied and Environmental Microbiology 37, 1016-1024.

Warembourg FR, R Dreesen, K Vlassak and F Lafont. 1987. Peculiar Effect of Azospirillum Inoculation on Growth and Nitrogen Balance of Winter Wheat (Triticum aestivum). Biology and Fertility of Soils 4, 55-59.

Watanabe I and C Lin. 1984. Response of Wetland Rice to Inoculation with Azospirillum lipoferum and Pseudomonas sp. Soil Science and Plant Nutrition 30, 117-124.

Yahalom, Y Kapulnik and Y Okon. 1984. Response of Setaria italica to Inoculation with Azospirillum brasilense as Compared to Azotobacter chroococcum. Plant Soil 82,77-85.


Refbacks

  • There are currently no refbacks.