PERUBAHAN KADAR KOLESTEROL SERUM PADA TIKUS SETELAH MENGKONSUMSI MALTOOLIGOSAKARIDA YANG DISINTESIS SECARA ENZIMATIK MENGGUNAKAN AMILASE Bacillus licheniformis BLl

Achmad Dinoto, Rita Dwi Rahayu, Aryani S Satyaningtijas
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Abstract

To investigate the impact of consuming enzymatically synthesized maltooligosaccharide using Bacillus licheniformis BLl amylase on reduction of serum cholesterol level, in preclinical study groups of Sprague Dawley rats were fed for 3 weeks with four different test diets: basal diet (BD), high serum cholesterol-stimulating basal diet (CD), high serum cholesterol-stimulating basal diet supplemented with 3% (w/w feed) commercial maltooligosaccharide (CI), and high serum cholesterol-stimulating basal diet supplemented with 3% (w/w feed) maltooligosaccharide synthesized by enzimatic reaction of B. licheniformis BLl amylase (CB).Our result shows a reduction of serum cholesterol level up to 39.1% in rats CB after three weeks consuming maltooligosaccharide synthesized using B. licheniformis BLl amylase. In addition, significantly increased blood glucose level was not found in this study as an impact of consuming maltooligosaccharide synthesized using B. licheniformis BLl amylase.

Keywords

maltooligosakarida, Bacillus licheniformis, kolesterol, tikus, uji praklinis.

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References

Ali M, S Mhiri, M Mezghani and S Bejar. 2001. Purification and Sequence Analysis of the Atypical Maltohexaose-Forming a-Amylase of the B. stearothermophilus US 100. Enzyme and Microbial Technology 28, 537-542.

Alles MS, NM de Roos, JC Bakx, E van de Llsdonk, PL Zock and G Hautvast. 1999. A Consumption of Fructooligosaccharides Does Not Favorably Affect. Blood Glucose and Serum Lipid Concentrations in Patients with Type 2 Diabetes. American Journal of Clinical Nutrition 69, 64-69.

Begley M, C Hill and CGM Gahan. 2006. Bile Salt Hydrolase Activity in Probiotics. Applied and Environmental Microbiology 72, 1729 - 1738.

Dinoto A, A Suksomcheep, S Ishizuka, H Kimura, S Hanada, Y Kamagata, K Asano, F Tomita and A Yokota. 2006. Modulation of Rat Cecal Microbiota by the Administration of Raffinose and EncapsulatedBifidobacterium breve. Applied and Environmental Microbiology 72, 784-792.

Doukyu N, W Yamagishi, H Kuwahara and H Ogino. 2008. A Maltooligosaccharide-Forming Amylase gene from Brachybacterium sp. Strain LB25: Cloning and Expression in Escherichia coli. Biosciences Biotechnology and Biochemistry 72, 2444-2447.

Fiordaliso M, N Kok, JP Desager, F Goethals, D Deboyser, M Roberfroid and N Delzenne. 2006. Dietary Oligofructose Lowers Triglycerides, Phospholipids and Cholesterol in Serum and Very Low Density Lipoproteins of Rats. Lipids 2, 163-167.

Hesta M, J Debraekeleer, GPJ Janssens and R De Wilde. 2001. The Effect of A Commercial High-Fibre Diet and An Iso-Malto-Oligosaccharide-Supplemented Diet on Post-Prandial Glucose Concentrations in Dogs. Journal of Animal Physiology and Animal Nutrition 85, 217-221.

Ikegami S., F Tsuchihashi, H Harada, N Tsuchihashi, E Nishide and S Innami. 1990. Effect of Viscous Indigestible Polysaccharides on Pancreatic-Biliary Secretion and Digestive Organs. Journal of Nutrition 120, 353-360.

Imaizumi K, Y Nakatsu, M Sato, Y Sedarnawati and M Sugano. 1991. Effects of Xylooligosaccharides on Blood Glucose, Serum and Liver Lipids and Cecum Short-chain Fatty Acids in Diabetic Rats. Agricultural and Biological Chemistry 55, 199-205.

Kurdi P, H Tanaka, HW van Veen, K Asano, F Tomita and A Yokota. 2003. Cholic Acid Accumulation and Its Diminution By Short-Chain Fatty Acids in Bifidobacteria. Microbiology 149, 2031 - 2037.

Kurdi P, HW van Veen, H Tanaka, I Mierau, WN Konings, GW Tannock, F Tomita and A Yokota. 2000. Cholic Acid is Accumulated Spontaneously, Driven by Membrane ApH, in Many Lactobacilli. Journal of Bacteriology 182, 6525-6528.

Levrat MA, SR Behr, C RSmesy and C Deminge. 1991a. Effects of Soybean Fiber on Cecal Digestion in Rats Previously Adapted to a Fiber-Free Diet. Journal of Nutrition 121, 672-678.

Levrat MA, C Rimisy and C Demlgne. 1991b. High Propionic Acid Fermentations and Mineral Accumulation in the Cecum of rats Adapted to Different Levels of Inulin. Journal of Nutrition 121, 1730-1737.

McLean RAH, MA Eastwood, WG Brydon, A Busuttil and LF McKay. 1984. A Study of the Effects of Dietary Gum Arabic in the rat. British Journal of Nutrition 51, 47-56.

Mln BC, SH Yoon, JW Kim, YW Lee, YB Kim and KW Park. 1998. Cloning of Novel Maltooligosaccharide- Producing Amylases as Antistaling Agents for Bread. Journal of Agricultural and Food Chemistry 46, 779-782.

Molis, C, B. Flourie, F. Ouarne, MF. Gailing, S. Lartigue, A. Guibert, F. Bornet and JP. Galmiche. 1996. Digestion, Excretion, and Energy Value of Fructooligosaccharides in Healthy Humans. American Journal of Clinical Nutrition 64, 324-328.

Momma M. 2000. Cloning and Sequencing of the Maltohexaose-producing Amylase Gene of Klebsiella pneumoniae. Bioscience, Biotechnology, and Biochemistry 64, 428-431.

Nagarajan D, G Rajagopalan and C Krishnan. 2006. Purification and Characterization of A Maltooligosaccharide-Forming Alpha-Amylase from A New Bacillus subtilis KCC103. Applied Microbiology and Biotechnology 73, 591-597.

Nakakuki T. 2003. Development of Functional Oligosaccharides in Japan. Trend in Glycoscience and Glycotechnology 15, 57-64.

Okemoto H, S Kobayashi, M Momma, H Hashimoto, K Hara and K Kainuma. 1986. Isolation and Cultivation of A Novel Microorganism Producing A Maltopentaose-Forming Enzyme. Applied Microbiology and Biotechnology 25, 137-142.

Oku T, T Tokunaga T and N Hosoya. 1984. Nondigestibility of A New Sweetener, "Neosugar," in the Rat. Journal of Nutrition 114, 1574-1581.

Rycroft CE, MR Jones, GR Gibson and RA Rastall. 2001. A Comparative In Vitro Evaluation of the Fermentation Properties of Prebiotic Oligosaccharides. Journal of Applied Microbiology 91, 878-887.

Sakuma K. 2002. Molecular Mechanism of the Effect of Fructooligosaccharides on Calcium Absroption. Bioscience Microflora 21, 13-20.

Takasaki Y. 1985. An Amylase Producing Maltotriose from Bacillus subtilis. Agricultural and Biological Chemistry 49, 1091-1097.

Takasaki Y, M Kitajima, T Tsuruta, M Nonoguchi, S Hayashi and K Imada. 1991. Maltotriose-Producing Amylase from Microbacterium imperiale. Agricultural and Biological Chemistry 55, 687-692.

Tanaka H, K Doesburg, T Iwasaki and I Mierau. 1999. Screening of Lactic Acid Bacteria for Bile Salt Hydrolase Activity. Journal of Dairy Science 82, 2530- 2535.

Tokunaga T, T Oku and N Hosoya. 1986. Influence of Chronic Intake of New Sweetener Fructooligosaccharide (Neosugar) on Growth and Gastrointestinal Function of the Rat. Journal of Nutritional Science and Vitaminology 32, 111-121.

Yoneyama M, T Mandai, H Aga, K Fujii, S Sakai, T Shintani, K Mou and Y Katayama. 1992. Effect of Lactosucrose Feeding on Cecal pH, Short-Chain Fatty Acid Concentration and Microflora in Rats. Nippon Etyo Shokuryo Gakkaishi 45, 109-115.


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