KERAGAMAN BAKTERI ENDOFITIK DnSOLASI DARI EMPAT VARIETAS PADI DENGAN METODA ARDRA

Dwi N Susilowati, Nurul Hidayatun, Tasliah Tasliah, K Mulya
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Abstract

Sixty eight endophytic bacteria were isolated from four different rice varieties (IR64, Cirata, Code and Limboto) obtained from agroecosystem in Cikembar, Sukabumi, West Java. Those isolates were subjected for analysis the diversity based on genetic fingerprinting through Amplified Ribosomal DNA Restriction Analysis (ARDRA) method. The objective of this research is to characterize the predominant endophyte bacteria are present within various rice varieties grown on agroecosystem in Cikembar, Sukabumi by using ARDRA method. The results shows that restriction analysis with both Rsal and HaelH was sufficient to allocate the endophyte bacteria from four different rice varieties into the 29 types. Moreover, Rsal alone was capable of resolving the 11 types, followed by HaelH 14 types. In general, the result may explain that there is no collinierity between the cluster and their host plant. Staphylococcus, Bacillus, Agrobacterium, Serratia, Klebsiella, Acidovorax and Pseudomonas were identified as endophytic bacteria from rice varieties in this agroecosystem based on their 16S rRNA sequences. Seven types were placed in close proximity to these genera, but other types were still unknown. Among these isolates, genera Staphylococcus and Bacillus are common to rice endophytes.

Keywords

keragaman, bakteri endofit, 16S ribosomal DNA, ARDRA

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References

Bacon CW, AE Glenn and DM Hinton. 2002. Isolation, in planta detection and culture of endophytic bacteria and fungi. In: CJ Hurst, RL Crawford, MJ Mclneraey, GR Knudsen and LD Stetzenbach (Eds.). Manual of Environmental Microbiology, 543-553. 2nd ed. ASM Press, Washington DC.

Bell CR, GA Dickie, WLG Harvey and JWYF Chan. 1995. Endophytic bacteria in grapevine. Can. J. Microbiol. 41, 46-53. Bottini R, F Cassan and P Piccoli. 2004. Gibberellin production by bacteria and its involvement in plant growth promotion and yield increase. Appl. Microbiol. Biotechnol 65, 497-503.

Compant S, B Duffy, J Nowak, C Clement and EA Barka. 2005. Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Appl. Environ.Microbiol. 71, 4951-959.

Elbeltagy A, K Nishioka, H Suzuki, T Sato, YI Sato, H Morisaki, H Mitsui and K Minamisawa. 2000. Isolation and characterization of endophytic bacteria from wild and traditionally cultivated rice varieties. Soil Sci. Plant Nutr. 46, 617629.

Feng Y, D Shen and Song W. 2006. Rice endophyte Pantoea agglomerons Y19 promotes host plant growth and affects allocation of host photosynthates. J. Appl. Microbiol. 100, 938-945.

Hallman J, A Quadt-Hallmann, WF Mahaffe and JW Kloepper. 1997. Bacterial endophytes in agricultural crops. Can. J. Microbiol. 43, 895-914.

Hallman J. 2001. Plant interaction with endophytic bacteria. In: MJ Jager and NJ Spence. Biotic Interaction in Plant-Pathogen Association. CBA, London.

Kobayasbi DY and JD Palumbo. 2000. Bacterial Endophytes and Their Effects on Plants and Uses in Agriculture. CW Bacon and JF White Jr (Eds.)- Marcel Dekker, New York.

Lazo GR, R Roffey and DW Gabriel. 1987. Conservation of plasmid DNA sequences and pathovar identification of strains of Xanthomonas campestris. Phytopathology 77, 448-453.

Lesmana OS, HM Toha dan I Las. 2003. Deskripsi Varietas Unggul Baru Padi. Balai Penelitian Tanaman Padi, Sukamandi.

Mano H, F Tanaka, C Nakamura, H Kaga and H Morisaki. 2007. Cultivable endophytic bacterial flora of the maturing leaves and roots of rice plants (Oryza saliva) cultivated in a paddy field. Microbes and Environment 22(2), 175-185.

Mano H and H Morisaki. 2008. Endophytic bacteria in the rice plant. Microbes and Environment 23(2), 109-117. McChang MR, AA Padheye and L Ajello. 1999. Storage of stock culture of filamentous fungi and yeast in sterile distillated Water. Applied Microbiology 35, 218-222.

Reiter B, U Pfeifer, H Schwab and A Sessitsch. 2002. Response of endophytic bacterial communities in potato plants to infection with Erwinia carotovora subsp. atroseptica. Appl. Environ. Microbiol. 68, 2261-2268.

Rodiyah. 2003. Distribusi dan Diversitas Genetik Bakteri Diazotrof Endofitik pada Tebu (Saccharum offwinarum L.). Tests. Program Pasca Sarjana UGM, Yogyakarta.

Sambrook J and DW Russel. 2001. Molecular Cloning: a Laboratory Manual. 3"1 Edition. Cold Spring Harbor Laboratory Press, New York.

Strobel G and B Daisy. 2003. Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews 67(4), 491-502.

Susilowati DN, A Akhdiya, K Mulya, £ Pratiwi, H Purwanti, A Suhendar, I Manzila, RD Hastuti, D Wahyuno, D Manohara, N Hidayatun, S Salma, Nurichana, S Soedjono, R Saraswati dan K Herlina. 2006. Laporan Hasil Penelitian. Konservasi dan Karakterisasi Mikroba Pertanian. Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor.

Weidner S, W Arnold and A Pukle. 1996. Diversity of uncultured microorganisms associated with the seagrass Halophila stipulacea estimated by retriction fragmenth length polymorphim analysis of gene encoding 16S rRNA and 23 S rRNA intergenic spacers, repetitive extragenic palindromic PCR genomic finger printing and partial 16S rRNA sequencing. Applied Environmental Microbiology 64, 2096-2104.

Zinniel DK, P Lambrecht, NB Harris, Z Feng, D Kuczmarski and P Higley. 2002. Isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. Appl. Environ. Microbiol. 68, 2198-2208.


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