INFEKSI Salmonella enteritidis PADA TELUR AYAM DAN MANUSIA SERTA RESISTENSINYA TERHADAP ANTIMIKROBA

Anni Kusumaningsih, M Sudarwanto
10.14203/beritabiologi.v10i6.1944
| Abstract views: 3095 | PDF views: 2556

Abstract

Salmonella enteritidis is one of pathogenic bacteria in chicken and human with re-emerging foodbome pathogen. Uncontrol use of antimicrobial drugs for the prevention and treatment of infectious diseases in chicken and human may lead to the development of antimicrobial resistance. Sampels of chicken eggs were collected from markets, layer farms, and grant parent stock farms. Sampels from human were anal swabs collected from hospitals and a laboratory of microbiology. Identification of bacteria was conducted by isolation and biotyping using selected media for Salmonella, while serotyping S. enteritidis was conducted with spesific 0 somatic (1, 9, and 12) and H flageIJa (m) antigen.Antimicrobial resistance tests were conducted by the standard diffusion method using antimicrobial disks.The results of isolation and identification of Salmonella show that 9 out of 122 (7.4%) sampels of layer eggs and 7 out of 23 (30.4%) sampels of embrioneted eggs were positive with Salmonella spp. The results of serotyping against S. enteritidis show that 7 out of 9 (77.0%) from layer eggs and 7 out of 7 (100.0%) from embrioneted eggs were positive with S. enteritidis.From the IS anal swabs of human were obtained 14 (93,3%) contain S. enteritidis.The antimicrobial resistance profiles of S. enteritidis isolated from layer eggs show high resistances to streptomycin (42.9%), neomycin (85.7%), doxicycline (64.3%), and ciprofloxacin (57,1%), whereas those S. enteritidis isolated from human show high resistances to streptomycin (50.0%), neomicyn (85.7%), tetracycline (42.9%), and doxicycline (42.9%).Multiple resistance profiles of S. enteritidis isolated from eggs were mostly to 2-3 antibiotics and those of S. enteritidis isolated from human were mostly to more than 5 antibiotics.

Keywords

Salmonella enteritidis, chicken egg, human, resistance, antimicrobe

Full Text:

PDF

References

Akhtar F, I. Hussain, A. Khan, and SU. Rahman. 2010. Prevalence anf Antibiogram Studies of Salmonella Enteritidis Isolated from Human and Poultry Sources. Pakistan Veterinary Journal. 30(l):25-28.

Altekruse, S.F., F.H. Hyman, K.C. Klontz, B.T. Timbo, and L.K. Tollefson. 1994. Foodborne Bacterial Infections in Individuals with the Human Immunodeficiency Virus. South MedicalJoumal. 87:169-73.

Altekruse SF, Cohen ML, and Swerdlow DL. 2m Perspective: Emerging Foodborne Diseases. Centers im. Diseases Control and Prevention. Atlanta. Georgia. Pp. 1-9.

Angulo FJ, Nunnery JA, and Blair HD. 2004. Antimicrao resistance in zoonotic enteric pathogens. Rrmm Sdentifique et Technique. International Office f Epizootics. 23(2):485-496.

Anon. 2002. Feed Additive Compendium. Vol. 41. The Nlife Publishing Company. Minnesota. USA.

Ahmed R, Soule G, Demezuk WH, Clark C, Khakhti E. Ratman S, Marshall S, Ng. Lai-King, Woodward DC Johnsons WM, Rodgers FG. 2000. Epidemioks Typing of Salmonella enterica Serotype Enteritidis Canada-wide Outbreak of Gastroenteritis due Contaminated Cheese. Journal of Clinical Microbiolog 38(6):2403-2406.

Barber DA, Miller GY, McNamura PE. 2003. Models of Antimicrobial Resistance and Foodborne Illness. "Examining Assumptions and Practical Applications' Journal of Food Protection. 66(4):700-709.

Bartlett CLR. 1996. An Overview of Emerging Foodborne an Waterborne Diseases. Eastern Medical Health Journal. 2 (1) :51-60

Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic Susceptibility Testing by a Standardised Single Disc Method. American Journal of Clinical Pathology. 45:493-496.

Bridson EY. 1998. The Oxoid Manual (8th ed). Oxoid Limited, Wade Road, Basingstoke, Hampshire, England. Burns JL. 1995. Mechanism of Bacterial Resistance. Pediatnc Clinical of North America. 42: 497-507.

[CDC] Centers for Diseases Control and Prevention. 2001. Salmonella enteritidis. Diseases Information, Division of Bacterial and Mycotic Diseases, http://www.cdc.gov/ncidod/dbmd/diseaseinfo/salmentg.htm. Januari 20,2003.

Courvalin P. 1994. Transfer of Antibiotic Resistance Genes between Gram-positive and Gram-negative Bacteria. Antimicrobial Agents of Chemotherapy. 37:855-869.

Cowan and Steel. 2003. Manual for the Identification of Medical bacteria. Second edition. Cambrige University Press.

Darmojono. 2001. Penyakit Tifus (Salmonellosis). Dalam: Penyakit Menular dari Binatang ke Manusia (1st). Milenia Populer. 111-121.

[EMEA] The European Agency for the Evaluation of Medical Products, Veterinary Medicine Evaluation Unit. 1999. Antibiotics resistance in The European Union associated with Therapetic Use of Veterinary Medicines. Report and Quantitative Risk assessment by the Committee for veterinary medicinal Products. 7 Westfeery Circuss, Canary Wharf. London. UK. Pp.:79

Furuya EY and Lowy FD. 2006. Antimicrobial-resistant Bacteria in The Community Setting. Microbiology. Nature Publishing Group. Nature Reviews. 4:36-45.

Gast RK and Benson ST. 1995. The Comparative Virulence for Chicks of Salmonella enteritidis PT 4 Isolates and isolalates of PT's Commonly Pound in Poultry in United States. Avian Diseases. 39:567-574.

Graham SM, Molyneux EM, Walsh AL, Cheesbrough JS, Molyneux ME, Hart CA. 2000. Nontyphoidal Salmonella Infection of Children in Tropical Africa. Pediatric Infectious Diseases Journal. 19:1189-1196.

Hang'ombe BM, Sharma RN, Skjerve E, Tuchili LM. 1999. Occurance of Salmonella Enteritidis in Pooled table Eggs and Market-ready Chicken Carcasses in Zambia. Reseach Note. Avian Diseases. 43:597-599.

Harada H, Sasaki T, Suginaka M, Kusuma T, Sugano K, Omuro Y. 1998. Database for Salmonella Contamination in Feeds (1979-1997). Bulletin of National Fertilizer Feed Insfecion. No.23. April.

Henzler DJ and Opitz HM. 1992. The Role of Mice in the Epizootiology of Salmonella Enteritidis Infection on Chicken layer Farm. Avian Diseases. 36:625-631.

Mishu B, Kohler J, and Lee LA. 1994. Outbreak of Salmonella enteritidis Infection in The United States. 1985-1991. Journal of Infectious Diseases. 169:457-552.

Moerad B. 2003. Pencemaran Salmonella spp. dalam produk pangan asal ternak dan kebijakan pemerintah dalam penanganan masalah keamanan pangan. Direktorat Kesehatan Masyarakat Veteriner. Direktorat Jenderal Produksi Petemakan. Disampaikan pada Simposium Sehari Purna Bakti "Teknologi Veteriner dalam Peningkatan Kesehatan Hewan dan Produknya". Balitvet. 12 Maret 2003.

Murray C. 1984. Salmonella. Report on consultancy by C. Murray. RIAD. Bogor. Indonesia. Nastasi A, Mammina C, Cannova L. 2000. Antimicrobial Resistance in Salmonella enteritidis, Southern Italy, 1990-1998. Emerging Infectious Diseases. 6(4):401-403.

[NCCLS] The National Committee for Clinical Laboratory Standards. 2002. Performance Standards for Antimicrobial Susceptibility Testing: Twelth International Supplement. Januari. M 100-S12. 22(1): 1-117.

Noor SM dan Poeloengan M. 2005. Pemakaian Antibiotika pada Ternak dan Dampaknya pada Kesehatan Manusia. Presiding Lokakarya Nasional Keamanan Pangan Produk Peternakan. Pusat Penelitian dan Pengembangan Peternakan. Badan Penelitian dan Pengembangan Pertanian. Bogor, 14 September 2005. 56-64.

Onifade AA, Babatunde GM. 1997. Comparative Response of Broiler Chickens to a High Fibre Diet Supplemented with Four Antibiotics. Animal Feed Science and Technology. 64(2/4): 337-342.

[OIE] Office International des Epizooties. 2000. Manual of Standards for Diagnostic Tests and Vaccines. List A and B Diseases of Mammals, Birds and Bees. World Organization for Animal Health. Pascual M, Hugas M, Badiola JJ, Monfort JM, Garriga M. 1999. Lactobacillus salivartius CTC 2197 Prevents Salmonella enteritidis Colonization in Chicken. Appllied Environmental Microbiology. 65(11):4981-4986.

Soewandojo E, Suharto, Hadi U. 1997. Typhoid Fever: Clinical Picture, Treatment and Status after Therapy. Proceeding of the Third Asia Pasific Symposium on Typhoid Fever and others Salmonellosis. Denpasar, Bali, Indonesia. Desember 8-10. Medical Journal of Indonesia. 7 (supp):95-104.

Tassios PT, Markogiannakis A, Vatopoulos AC, Katsanikou E, Velonakis EN, Kourea-Kremastinou J, Legakis NJ. 1997. Molecular Epidemiology of Antibiotic Resistance of Salmonella enteritidis during a 7-Year in Greece. Journal of Clinical Microbiology. 35(6): 1316-1321.

Tjaniadi P, Lesmana M, Subekti D, Machpud N, Komalarini S, Santoso W, Simanjuntak CH, Punjabi N, Campbell J, Alexander WK, Beecham III HJ, Corwin AL, Oyofo BA. 2003. Antimicrobial resistance of Bacterial Pathogens Associated with Diarrheal Patients in Indonesia. American Journal of Tropical Medicine. 68(6):666-670.

Thrusfield M. 2007. Presenting Numerical Data. Dalam: Veterinary Epidemioloy. 3th. Ed. Blackwell Publishing Company. University of Edinburgh. UK. Pp. 214-224.

Villa L, Mammina C, Miriagou V, Tzouvelekis LS, Tassios FT, Nastasi A, Carattoli A. 2002. Multidrug and broadspectrum Cephalosporin Resistance among salmonella enterica Serotype Enteritidis Clinical Isolates in Southern Italy. Journal of Clinical Microbiology. 40(7):2662-2665.

[WHO] World Health Organization. 2000. World Health Organization Global Principles for the Containment of Antimicrobial resistance in Animals Intended for Food. "WHO Departement of Communicable Diseases Surveillance and Response.

DOI: https://doi.org/10.14203/beritabiologi.v10i6.1944

Refbacks

  • There are currently no refbacks.