SIFAT PROTEKSI EKSTRAK AIR PANAS TEH {Camellia sinensis (L.) Kuntze} HIJAU PADA KHAMIR Candida tropicalis YANG DIPERLAKUKAN DENGAN PARACETAMOL

Heddy Julistiono
| Abstract views: 304 | PDF views: 412

Abstract

In order to develop yeast Candida tropicalis as a model cell for evaluation of substance having anti- or pro-oxidant activity in cell level, the effect of hot water tea {Camellia sinensis (LJ Kuntze) extract on peroxidized lipids, a marker of oxidative stress and cell mortality in the yeast caused by paracetamol has been evaluated. Incubation of yeast cell in the presence of 1.38 % green tea for 2 h decreased cell viability followed with increasing of peroxidized lipids, whereas 0.27 % green tea caused increasing of peroxidized lipids without causing cell death.Yeast cell was not affected by 0.1 % green tea. Incubation of yeast cell with presence of 0.15 % (g/v) paracetamol for 2 h did not cause cell mortality, however content of peroxidized lipids increased significantly. In the presence of higher dose of paracetamol (0.3 %) cell viability remarkably decreased and followed with increasing of peroxidized lipids significantly. Green tea of 0.1 % increased cell viability of cells treated with 0.3 % paracetamol while peroxidised lipids decreased. The data indicated that high dose of paracetamol caused oxidative stress in yeast cells, while green tea with lower concentration might protect paracetamol toxicity due to its antioxidant property. Although the antioxidant property, high dose of green tea could cause oxidation due to its pro-oxidant activity. In conclusion, yeast C. tropicalis could be potentially used as a model cell to evaluate substances having antioxidant property in cell level.

Keywords

Green tea {Camellia sinensis (L.) Kuntze). Candida tropicalis, paracetamol, antioxidant, Reactive Oxygen Species

Full Text:

PDF

References

Almeida B, A Silva, A Mesquita, B Sampaio-Marques, F Rodrigues and Ludovico P. 2008. Drug-induced apoptosis in yeast, Biochimica et Biophysica Ada 1783, 1436- 1448.

Babich H, AG Schuck, JH Weisburg and HL Zuckerbraun. 2011. Research strategies in the study of the pro-oxidant nature of polyphenol nutraceuticals. Journal of Toxicology 2011, 467305. Epub 2011 Jun 26.

Balentine DA, SA Wiseman and LC Bouwens. 1997. The chemistry of tea flavonoids. Critical Reviews in Food Science and Nutrition 37,693-704.

Cotelle N. 2001. Role of flavonoids in oxidative stress. Current Topics in Medicinal Chemistry 1, 569-590.

Craft DL, KM Madduri, M Eshoo and CR Wilson. 2003. Identification and characterization of the CYP52 family of Candida tropicalis ATCC 20336, important for the conversion of fatty acids and alkanes to alpha, omegadicarboxylic acids. Appl Environ Microbiol. 69, 5983-5991.

Gonzalez FJ. 2005. Role of cytochromes P450 in chemical toxicity and oxidative stress: studies with CYP2E1. Mutation Research 569,101-110.

De Winde JH, JM Thevelein and J Winderickx. 1997. From feast to famine: adaptation to nutrient depletion in yeast. In: S Hofmann and WH Mager (Eds). Yeast Stress Respons, 1-52. Springer-Verlag. Berlin.

He F and YT Chen. 2005. Cloning and heterologous expression of the NADPH cytochrome P450 oxidoreductase genes from an industrial dicarboxylic acid-producing Candida tropicalis. Yeast 22,481-491.

Hirasawa M and K Takada. 2004. Multiple effects of green tea catechin on the antifungal activity of antimycotics against Candida albicans. Journal of Antimicrobial Chemotherapy 53,225-229.

Julisriono H. 2006. Superoxide Dismutase and Ethanol Resistance by Sodium Chloride and Lead in Yeast Candida Y390. Jurnal Biologi Indonesia 4,1-7.

Kapiotis S, G Sengoelge, M Hermann, I Held, C Seelos and BMK Gmeiner. 1997. Paracetamol catalyzes myeloperoxidase-initiated lipid oxidation in LDL. Arteriosclerosis, Thrombosis, and Vascular Biology 17, 2855-2860.

Lamberta JD and RJ Eliasa. 2010. The antioxidant and prooxidant activities of green tea polyphenols: a role in cancer prevention. Archive of Biochemistry and Biophysics 501,65-72.

Liu S, C Li, L Xie and Z Cao. 2003. Intracellular pH and metabolic activity of long-chain dicarylic acid-producing yeast Candida tropicalis. Journal of Bioscience and Bioengineering 96,349-53.

Lu N, P Chen, Q Yang and YY Peng. 2011. Ami- and prooxidant effects of (+)-catechin on hemoglobin-induced protein oxidative damage. Toxicology In Vitro 25,833-838.

Maeta K, W Nomura, Y Takatsume, S Izawa and Y Inoue. 2007. Green tea polyphenols function as prooxidants to activate oxidative-stress-responsive transcription factors in yeasts. Applied and Environmental Microbiology 73, 572-580.

Manon S. 2004. Utilization of Yeast to investigate the role of lipid oxidation in cell death. Antioxidants & Redox Signaling 6,259-267.

Ojo OO, FR Kabutu, M Bello and U Babayo. 2006. Inhibition of paracetamol-induced oxidative stress in rats by extracts of lemongrass (Cymbropogon citratus) and green tea (Camellia sinensis) in rats. African Journal of Biotechnology 5, 1227-1232.

Oz HS, CJ McClain, HT Nagasawa, MB Ray, WJ de Villiers and TS Chen. 2004. Diverse antioxidants protect against acetaminophen hepatotoxicity. Journal of Biochemical and Molecular Toxicology 18,361-368.

Prescott, LF. 1983. The treatment of acetaminophen poisoning. Drugs 25, 290-314 Ray A, SR Chaudhuri, B Majumdar and SK Bandyopadhyay. 2002. Antioxidant activity of etanol extract of rhizome of Picrorhiza Kurroa on indomethacin induced gastric ulcer during healing. Indian Journal of Clinical Biochemistry 17, 44-51

Saono S and I Gandjar. 1974. Hydrocarbon-utilizing soil yeasts from oil fields in Tjepu region (Central Java), Indonesia. Annales Bogorienses V, 123-129.

Shin BC, HH Ryu, JH Jong, BR Lee and HL Kim. 2009. The protective effects of green tea extract against L-arginine toxicity to cultured human mesangial cells. Journal of Korean Medical Science 24, S204-S209.

Srikanth CV, AK Chakraborti and AK Bachhawat. 2005 Acetaminophen toxicity and resistance in the yeast Saccharomyces cerevisiae. Microbiology 151,99-11.

Yulineri T dan H Julistiono. 2003. Penggunaan keragaman khamir berdasarkan keberadaan enzim superoksid dismutasenya untuk bioesai stres oksidatif. berkala Penelitian Hayati 8, 49-51.

Venkateswara B, Sirisha K and Chava VK. 2011. Green tea extract for periodontal health. Journal of Indian Society of Periodontology 15, 18-22.

Vermeulen NPE, JGM Bessems, R van de Streat (1992).Molecular aspects of paracetamol-induced hepatotoxicity and it mechanism based prevention. Drug Metabolism Reviews 24,367-407.


Refbacks

  • There are currently no refbacks.