PATOGENESITAS Streptococcus agalactiae DAN Streptococcus iniae PADA IKAN NILA (Oreochromis niloticus)

Dudung Daenuri, Walson Halomoan Sinaga
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Abstract

The objectives of this study are to test the pathogenicity of Streptococcus agalactiae and Streptococcus iniae in Nile Tilapia(Oreochromis niloticus) A challenge test was carried out in the Laboratory of Semarang Fish Quarantine. The method used in this study was the Completely Randomize Design(CRD) with three bacterial treatments Streptococcus agalactiae, Streptococcus iniae and control, three dose  107 ,108 , 109 with three replications for each treatment. The observed parameters include identification and reisolation of Streptococcus agalactiae, Postulat Koch test, survival rate (SR), and clinical symptoms. The result of this research showed that the survival rate of Nile Tilapia which has challenged by Streptococcus agalactiae was lower than that of Nile Tilapia which has challenged by Streptococcus iniae.

Keywords

Postulat Koch, LC50, Streptococcus agalactiae, Streptococcus iniae, Nile tilapia

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References

Arifin OZ, E Nugroho dan R Gustiano. 2007. Keragaman genetik populasi Ikan Nila (Oreochromis niloticus) dalam program seleksi berdasarkan RAPD. Berita Biologi 8(6), 465–471.

Austin B and DA Austin. 2007. Bacterial Fish Pathogens. Disease of Farmed and Wild Fish. 4th Edition. Springer, Praxis Publishing. Colchester, UK.

Chang PH and JA Plumb. 1996. Effect of salinity on Streptococcus infection of Nile Tilapia, Oreochromis niloticus. Journal of Applied Aquaculture 6, 39-45. Direktorat Jenderal Perikanan. 1991. Budidaya Ikan Nila. Pusat Penelitian dan Pengembangan Perikanan. Departemen Pertanian. Jakarta.

Duremdez R, A Al-Marzouk, JA Qasem, A Al-Harbi and H Gharabally. 2004. Isolation of Streptococcus agalactiae from cultured of silver pomfret, Pampus argenteus (Euphrasen), in Kuwait. Journal Fish Diseases 27, 307–310.

Eldar A, Y Bejerano and H Bercovier. 1994. Streptococcus shiloi and Streptococcus difficile: Two new streptococcal species causing a meningoencephalitis in fish. Current Microbiology 28, 139-143.

Eldar A, Y Bejerano, A Livoff, A Horovitcz and H Bercovier. 1995. Experimental sStreptococcal meningo-encephalitis in cultured fish. Veterinary Microbiology 43(3), 33-40.

Evans JJ, DJ Pasnik, PH Klesius, S Alablani. 2006. First report of Streptococcus agalactiae and Lactococcus garvieae from a wild bottlenose dolphin (Tursiops truncates). Journal of wildlife Diseases 42, 561-569.

Evans JJ, PH Klesius, PM Gilbert, CA Shoemaker, MA Al-Sarawi, J Landsberg, R Duremdez, A Al- Marzouk and S Al-Zenki. 2002. Characterization of beta-haemolytic group B Streptococcus agalactiae in cultured seabream. Sparus auratus and Wild Mullet, Liza klunzingeri (Day), in Kuwait. Journal of Fish Disease. 25, 505-513.

Evans JJ, AA Wiedenmayer, PH Klesius and CA Shoemaker. 2004. Survival of Streptococcus agalactiae from frozen fish following natural and experimental infections. Aquaculture 233, 15-21.

Figueiredo HPC, DO Carneiro, FC Faria and GM Costa. 2006. Streptococcus agalactiae associado a meningoencefalite e infeccao sistemica em Tilapiado- nilo (Oreochromis niloticus) no Brasil. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 58, 678-680.

Gustiano R, OZ Arifin dan E Nugroho. 2008. Perbaikan pertumbuhan ikan Nila (Oreochromis niloticus) dengan seleksi famili. Media Akuakultur 3(2), 98- 106.

Halt JG. 1994. Bergey’s Manual of Determinative Bacteriology. 9thEdition. William & Wilkins. Baltimore,Maryland, USA.

Hubert JJ. 1980. Bioassay. Kendal/Hunt Publishing Company. Dubuque, Iowa, USA.

Inglis V, RJ Roberts and NR Bromage. 1993. Bacterial Disease of Fish. Institute of Aquaculture. Oxford. Blackwell Scientific Publications. London, Edinburg, Boston, Melbourne, Paris, Berlin, Vienna.

Murwantoko. 2007. Materi Pelatihan Bakteriologi UGM. Fakultas Pertanian Jurusan Perikanan-Universitas Gajah Mada, Yogyakarta. Nugroho E dan Maskur. 2002. Benarkah ikan Nila Merah adalah hasil hybrid? (melacak asal usul nila merah dengan menggunakan molecular genetic markers). Warta Penelitian Perikanan Indonesia 8(1), 8 – 11.

Popma TJ and M Masser. 1999. Tilapia: Life History and Biology. SRAC Publication Thailand. Rasheed V and JA Plumb. 1984. Pathogenicity of a nonhemolityc group B Streptococcus sp. in gulf killifish (Fundulus grandis Baird and Girad). Aquaculture 37, 97-105.

Salvador R, EE Muller, JH Leonhardt, LG Pretto-Giordano, JA Dias, JC Freitas and AM Moreno. 2003. Isolamento de Streptococcus spp de Tilapia do Nilo (Oreochromis niloticus) e qualidade da agua de tanques rede na regiao Norte do Estado do Parana, Brasil. Semina: iencias Agrarias 24, 35-42.

Salvador R, EE Muller, JC Freitas, JH Leonhardt, LG Pretto-Giordano, and JA Dias. 2005. Isolation and characterization of Streptococcus spp. group B in Nila Tilapia (Oreochromis niloticus) reared in hapas nets and earth nurseries in the northern region of Parana State, Brasil. Cienca Rural 35, 1374-1378.

Yanong RPE and R Francis-Floyd. 2002. Streptococcal Infections of Fish. Circular FA-57. University of Florida IFAS Cooperative Extension Service.


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