PENGARUH MEDIA DASAR MS DAN N TERHADAP PERKEMBANGAN EMBRIO 6 SOMATIK PADA KULTUR MERISTEM JAHE (Zingiber officinale Rosc.)

Otih Rostiana, Sitti Fatimah Syahid
10.14203/beritabiologi.v8i5.1899
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Abstract

This study was performed to evaluate the development of somatic embryo from embryogenic calli of ginger meristem culture. Completely randomized design was applied, replicated 4 times. Embryogenic calli from meristem tissue of inner shoot bud of rhizome obtained on MS medium containing 100 mg/L glutamine, 2% sucrose with the addition of 1.0 mg/L 2,4-D and 3.0 mg/L BA, were subjected to proliferation medium, MS and N basal media containing 3% mannitol. Then, transferred into somatic embryo maturation medium, either MS or N basal media supplemented with 6% sucrose. The number of somatic embryos-formed significantly affected by the proliferation medium applied. The highest number of somatic embryos (about 82.0 per 1 g friable calli) was achieved on the MS medium, 4 weeks after incubation. In addition, the optimum growth of embryogenic calli containing somatic embryos was obtained on MS and N medium supplemented with 6% sucrose. There were significantly difference between the media applied (MS and N ) to somatic embryos maturation. The highest number of mature somatic embryos (57.2 embrios) was achieved on the MS medium, 18 days after incubation.

Keywords

Zingiber officinale, kultur meristem, proliferasi kalus, embrio somatik, medium MS, medium N . 6

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References

Adkins SW, AL Adkins, CM Ramage and RR Williams. 2002. In vitro ecology: Modification of headspace and medium conditions can optimize tissue and plant development. In: The Importance of Plant Tissue Culture and Biotechnology in Plant Sciences, 55-77. A Taji and R Williams (Eds). University of New England, Australia.

Anbazhagan VR and A Ganapathi. 1999. Somatic embryogenesis in cell suspension cultures of pigeonpea (Cajanus cajan). Plant Cell, Tissue and Organ Cult. 56, 179-184.

Bach A and B Pawlowska. 2003. Somatic embryogenesis in Gentiana pneumonanthe L. Acta Bio. Crac. Series Bot. 45 (2), 79-86.

Bhojwani SS and MK Razdan. 1996. Plant Tissue Culture: Theory and Practice. Elsevier Amsterdam, Oxford, New York, Tokyo.

Biro Pusat Statistik. 2003. Statistik Perdagangan Luar Negeri Indonesia. Biro Pusat Statistik, Jakarta.

Biro Pusat Statistik. 2004. Statistik Tanaman Obat-obatan dan Hias. Badan Pusat Statistik, Jakarta.

Bogunia H and L Przywara. 2000. Effect of carbohydrates on callus induction and regeneration ability in Brassica napus L. Acta Bio. Crac. Series Bot. 42 (1), 79-86.

Bronsema FBF, WJE van Oostveen and AAM van Lammeren. 1997. Comparative analysis of callus formation and regeneration on cultured immature maize embryos of the inbred lines A188 and A632. Plant Cell, Tissue and Organ Cult. 50, 57-65.

Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, YC Chu and Ing Bi, 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments of the nitrogen sources. Sci. Sin. 18, 650-668.

Davies PJ. 1995. The plant hormones: Their nature, occurrence and functions. In: Plant Hormones: Physiology, Biochemistry and Molecular Biology. PJ Davies (Ed) Kluwer Academic, Dordrecht, Boston, London.

Dedicova B, A Hricova, J Samaj, B Obert, M Bobak and A Pretöva. 2000. Shoots and embryo-like structures regenerated from cultured flax (Linum usitatissimum L.) hypocotyl segments. J. Plant Physiol. 157, 327-334.

Ditjenbun. 2004. Statistik Perkebunan Indonesia. Direktorat Jenderal Bina Produksi Perkebunan, Departemen Pertanian, Jakarta.

El Hadrami I, MP Carron and J Däuzac. 1991. Influence of exogenous hormones on somatic embryogenesis in Hevea brasiliensis. Ann. Bot. 67, 511-515.

Evans DE and Sharp WR. 1986. Somaclonal and gametoclonal variation. Dalam: Hand Book of Plant Cell Culture, Vol. 4, Technique and Application, 97- 132. Evans et al. (Eds) MacMillan Pub. Co., New York.

Fransz, PF and JHN Schel. 1991. Cytodifferentiation during the development of friable embryogenic callus in maize (Zea mays). Can. J. Bot. 69, 26-33.

Furlong NE, EA Lovelace and KL Lovelace. 2000. Research Methods and Statistics an Integrated Approach. Harcourt College, Tokyo.

George EF. 1993. Plant Propagation by Ttissue Culture. 2nd ed. Exegetics Ltd, England. George EF and PD Sherrington. 1984. Plant Propagation by Tissue Culture. Exegetics Ltd, England.

Guerra MP and W Handro. 1998. Somatic embryogenesis and plant regeneration in different organs of Euterpe edulis Mart. (Palmae) Control and structural features. J. Plant Res. 111, 65-71.

Guo Y and Z Zhang. 2005. Establishment and plant regeneration of somatic embryogenic cell suspension cultures of the Zingiber officinale Rosc. Sci. Hortic.107, 90-96.

Heinze B and J Schmidt. 1995. Monitoring genetic fidelity vs somaclonal variation in Norway Spruce (Picea abies) somatic embryogenesis by RAPD analysis. Euphytica 85, 341-345.

Jimenez VM. 2001. Regulation of in vitro somatic embryogenesis with emphasis on the role of endogenous hormones. R. Bras. Fisiol. Veg. 13 (2), 196-223.

Krikorian AD. 1995. Hormones in tissue culture and micropropagation. In: Plant Hormones: Physiology, Bochemistry and Molecular Biology. PJ Davies(Ed.). Kluwer Academic, Dordrecht, Boston, London.

Mariska I. 1997.Embriogenesis somatik tanaman kehutanan. Seminar Intern Balitbio, 27 Juni 1997.

Mariska I dan SF Syahid. 1992. Perbanyakan vegetatif melalui kultur jaringan pada tanaman jahe. Buletin Littri 4, 1-5.

Merkle SA, WA Parrott and EG Williams. 1990. Application of somatic embryogenesis and embryo cloning. In: Plant Tissue Culture: Applications and Limitations. SS Bhojwani (Ed). Elsevier, Amsterdam, Oxford, New York, Tokyo.

Narayanaswamy S. 1990. Plant Cell and Tissue Culture. Tata McGraw-Hill, New Delhi.

Oropeza M, AK Marcano and E de Garcia. 2001. Proteins related with embryogenic potential in callus and cell suspensions of sugarcane (Saccharum sp.). In vitro Cell Dev. Biol. Plant. 37, 211-216.

Padmanabhan D, DJ Cantliffe and RC Harrell. 1998. A comparison of shoot-forming and non-shoot-forming somatic embryos of sweet potato [Ipomoea batatas (L.) Lam.] using computer vision and histological analyses. Plant Cell Rep. 17, 685-692.

Percy RE, K Klimaszewska and DR Cyr. 2000. Evaluation of somatic embryogenesis for clonal propagation of western white pine. Can. J. For. Res. 30, 1867-1876.

Raghavan V. 2003. One hundred years of zygotic embryo culture investigations. In Vitro Cell. Dev. Biol. Plant. 89, 437-442.

Rahman MM, MN Amin, T Ahamed, MR Ali and A Habib. 2004. Efficient plant regeneration through somatic embryogenesis from leaf base-derived callus of Kaempferia galanga L. Asian J. of Plant Sci. 3 (6), 675-678.

Ramage CM and RR Williams. 2002. Mineral nutrition and plant morphogenesis. In: Vitro Cell. Dev. Biol. Plant. 38, 116-124.

Sakhanokho HF, Zipf A, K Rajasekaran, S Saha, GC Sharma and PW Chee. 2004. Somatic embryo initiation and germination in diploid cotton (Gossypium arboreum L.). In Vitro Cell. Dev. Biol. Plant. 40, 177-181.

Sass JE. 1951. Botanical Microtechnique. Iowa State University Press, Ames. Sitinjak RR, O Rostiana, T

Supriatun dan Karyono. 2006. Pengaruh 2,4-D dan BA terhadap induksi kalus embriogenik pada kultur meristem jahe (Zingiber officinale Rosc.). Berita Biol. 8 (2), 115-120.

Supriadi, JG Elphinstone, SJ Eden-Green and SY Hartati. 1995. Physiological, serological and pathological variation amongst isolates of Pseudomonas solanacearum from ginger and other hosts in Indonesia. J. Littri. 1(2), 88-98.

Talwar M and A Rashid. 1989. Somatic embryo formation from unemerged inflorescences and immature embryos of a graminaceous crop Echinochloa. Ann. Bot. 64, 195-199

Tan SK, R Pippen, R Yusof, H Ibrahim, N Rahman and N Khalid. 2005. Simple one-medium formulation regeneration of fingerroot [Boesenbergia rotunda (L) Mansf. Kulturpfl.] via somatic embryogenesis. In Vitro Cell. Dev. Biol-Plant 41, 757-761.

Torres AC, N Mfëe Ze and DJ Cantliffe. 2001. Abscisic acid and osmotic induction of synchronous somatic embryo development of sweet potato. In vitro Cell. Dev. Biol. Plant. 37, 262-267.

Van Creij MGM, DMF Kerckhoffs, SM de Bruijn, D Vreugdenhil and JM Van Tuyl. 1999. The effect of medium composition on ovary-slice culture and ovule culture in intraspecific Tulipa gesneriana L. crosses. Available at http://www.liliumbreeding.ne/crey-med.htm [12/06/2004].

Vasil IK, V Vasil, C Lu, P Ozias-Akins, Z Haydu and D Wang. 1982. Somatic embryogenesis in cereals and grasses In: Variability in Plants Regenerated from Tissue Culture. ED Earle and Y Demarly (Eds.) Praeger Scientific, New York, USA.

Wiweger M. 2003. Signal molecules in embryogenesis of norway spruce. Doctoral Thesis. Swedia University Agricultural Sciences, Uppsala.

Ziv M. 1999. Developmental and structural patterns of in vitro plants. In: Morphogenesis in Plant Tissue Culture. W Soh and SS Bhojwani (Eds.). Kluwer Academic, Dordrecht, Boston, London.

DOI: https://doi.org/10.14203/beritabiologi.v8i5.1899

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