Yulia Atika MS, I Made Artika, Novik Nurhidayat
| Abstract views: 350 | PDF views: 500


One of the constrains in the conversion process of biomass to bioethanol is the unoptimum technology for the conversion process.One of the causes is the difference between optimum temperature for enzymatic hydrolytic saccharification and for fermentation.Enzymatic hydrolysis requires high temperature and acidic pH, while fermentation operates at mild condition.Hence, thermophilic fermentative microbes are needed so that simultaneous saccharification and fermentation processes can be carried out.Alcoholdehydrogenase(ADH), an enzyme functions in the final step of fermentation, catalyzes reduction of acetaldehydeto ethanol so that it being one of indicator fermentative microbial.Protium javanicum, a typical fruit from Lombok grow at high temperature and has sour taste so that it predicted has a thermo acidophilic ADH.This study was aimed toidentify gene encoding ADHin Protiumjavanicum(Pjv) microbial isolates.ADH gene identification was carried out by DNA amplification using spesific adh primers inqPCR machine. Results showed that all isolates tested had adh gene and that of Bacillus sp-Pjv was the most efficiently amplified. Results of fermentationtest also showed that the Bacillus sp-Pjv isolate was a better ethanol-producer than the others.


alcohol dehydrogenase, thermoacidophilic, Bacillus sp-Pjv.

Full Text:



Arshadi and Grundberg. 2011. Biochemical Production of Bioethanol 199-216.Handbook of Biofuels Production. Woodhead Publishing. Sweden.

Bhalla A, B Namita, K Sudhir, K Bischoff and KS Rajesh. 2013. Improved lignocellulose conversion to biofuels with thermophilic bacteria and thermostable enzymes. Journal of Bioresource Technology 128, 751–759.

Bischoff KM, N Skinner and T Leathers. 2007. Antimicrobial susceptibility of Lactobacillus species isolated from commercial ethanol plants. J. Industrial Microbiol Biotechnology 34, 739-744.

Ceccarelli C, L Zhao-Xun, S Michael, P Gerd, MG Barry, PK Judith and B Brian. 2004. Crystal Structure and Amide H/D Exchange of Binary Complexes of Alcohol Dehydrogenase from Bacillus stearothermophilus: Insight into Thermostability and Cofactor Binding. Journal of Biochemistry, 43.

Cripps R, K Eley, DJ Leak , B Rudd, M Taylor, M Todd, S Boakes, S Martin, and T Atkinson. 2009. Metabolic engineeringof Geobacillusthermoglucosidasius for highyield ethanol production. Journal of metabolic engineering11, 398-40.

Extance J, SJ Crennell, K Eley, R Cripps, DW Hough and MJ Danson. 2013. Structure of a bifunctional alcohol dehydrogenase involved in bioethanol generation in Geobacillus thermoglucosidasius. Journal of Acta Crystallographica Section D Biological Crystallography 69, 2104-2115.

Hild HM, DC Stuckey and DJ Leak. 2003. Effect of nutrient limitation on product formation during continuous fermentation of xylose with thermoanaerobacter ethanolicus JW200 Fe (7). Journal of Applied Microbiology and Biotechnology 60, 679-686.

Littlechild JA, MN Isupov and JE Guy. 2003. Hyperthermophilic Dehydrogenase Enzymes. Journal of Biochemical Society Transactions 32, part 2.

Ou M, N Mohammed, LO Ingram and KT Shanmugam. 2009. Thermophilic Bacillus coagulansRequires Less Cellulases for Simultaneous Saccharification and Fermentation of Cellulose to Products than Mesophilic Microbial Biocatalysts. Journal of Applied Bioochemistry and Biotechnology 155, 76-82.

Quintero Y, M Poblet, JM Guillamo and A Mas. 2008. Quantification of the expression of reference and alcohol dehydrogenase genes of some acetic acid bacteria in different growth conditions. Journal of Applied Microbiology 766, 1365-2672.

Riyanti I. 2010. Beberapa Gen pada Bakteri yang Bertanggung Jawab terhadap Produksi Bioetanol. Jurnal Litbang Pertanian Bogor. 30, 41-47.

Takami H, Y Takaki, G Chee, S Nishi, Shigeru, H Suzuki, S Matsui and I Uchiyama. 2004. Thermodaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus. Oxford Journal 32, 6292-6303.

Zaldivar J, J Nielson and Olsson. 2001. Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration. J. Appl Microbiol Biotechnology 56, 17-34.

Zeikus GJ, C Vicille and Savchenko. 1998. Thermozymes: Biotechnology and Structur function. Journal of Extremophiles 2, 179-183.


  • There are currently no refbacks.