RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS

Iskandariah Iskandariah, Dinar Tri Soelistyowati, Rudhy Gustiano, Irin Iriana Kusmini, Gleni Hasan Huwoyon
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Abstract

The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan.

Keywords

Genetics, Kalimantan, morphometric truss, RAPD, Trichopodus pectoralis.

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References

Anonim. 2013. http://id,wikipedia,org/wiki/Sepat_siam. [29 April 2013].

Arifin OZ dan T Kurniasih. 2007. Karakterisasi morfologi keturunan pertama ikan Nila (Oreochromis niloticus) GET dan GIFT berdasarkan metode truss morphometrics. Jurnal Riset Akuakultur 2, 377 – 387.

Asih S, E Nugroho, AH Kristanto, dan Mulyasari. 2008. Penentuan variasi genetik ikan Batak (Tor soro) dari Sumatera Utara dan Jawa Barat dengan metode analisis Random Amplified Polymorphism DNA (RAPD). Jurnal Riset Akuakultur 3, 91 – 97.

Blezinsky VJ and RW Doyle. 1987. A morphometrics criterion for sex discrimination in Tilapia. In: RSV Pullin, Bhukaswan T, Tonguthai K, Maclan JL (Eds). 15, 439-444. The second international symposium on Tilapia in aquaculture. ICLARM Conference Proceeding. Philiphines.

Dunham RA. 2004. Aquaculture and fisheries biotechnology: genetic approach, 85 – 99. CABI Publishing Cambridge. USA.

Kusmini II, R Gustiano, dan Mulyasari. 2010. Karakterisasi truss morfometrik ikan Tengadak (Barbonymus schwanenfeldii) asal Kalimantan Barat dengan ikan Tengadak Albino dan ikan Tawes asal Jawa Barat. Prosiding Forum Inovasi Teknologi Akuakultur (FITA) 2010, 507 – 513.

Lante S, A Tenriulo, dan NN Palinggi. 2012. Variasi genetik ikan Beronang (Siganus guttatus) asal perairan Barru, Lampung dan Sorong menggunakan penanda RAPD (Random Amplified Polymorphism DNA). Jurnal Riset Akuakultur 7, 195 – 204.

Liu Z. 2007. Randomly amplified polymorphism DNA (RAPD). In: Aquaculture genome technologies. Blackwell Publishing, USA.

Mamangkey JJ, Sulistiono, DS Sjafei, D Soedarma, S Sukimin, dan E Nugroho. 2007. Keragaman genetik ikan endemik Butini (Glossogobius matanensis) berdasarkan penanda Random Amplified Polymorphism DNA (RAPD) di danau Towuti Sulawesi Selatan. Jurnal Riset Akuakultur 2, 389 – 397.

Mulyasari. 2010. Karakteristik fenotipe morfomeristik dan keragaman genotipe RAPD (Random Amplified Polymorphism DNA) ikan Nilem (Osteochilus hasselti) di Jawa Barat, 70 hal. [Thesis], Pascasarjana IPB. Bogor.

Miller MP. 1997. Tools For Population Genetic Analysis (TFPGA) version 1,3, 30 pp Department of Biological Science, Northern Arizona University, Arizona, USA.

Nei M and F Tajima. 1981. DNA polymorphism detectable by resctriction endonuclease. Genetics 97, 146 -163.

Nugroho E, M Takagi, and N Taniguchi. 1997. Practical manual on detection of DNA polymorphism in fish population study. Bulletin of Marine Sciences and Fisheries, 17, 109 – 129.

Nugroho E, W Hadie, J Subagja, dan T Kurniasih. 2005. Keragaman genetik dan morfometrik pada ikan Baung, Mystus nemurus dari Jambi, Wonogiri dan Jatiluhur. Jurnal Penelitian Perikanan Indonesia 11, 1 – 6.

Nugroho E, J Subagja, S Asih, dan T Kurniasih. 2006. Evaluasi keragaman genetik ikan Kancra dengan menggunakan marker mt DNA D-loop dan Random Amplified Polymorphism DNA (RAPD). Jurnal Riset Akuakultur 1, 211 – 217.

Sugama K, Haryanti, dan F Cholik. 1996. Bio-chemical genetics of tiger shrimp Penaeus monodon description electrophoresis detectable loci. Indonesian Fisheries Research Journal II, 19 – 28.


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