TIGA SPESIES PERONOSCLEROSPORA PENYEBAB PENYAKIT BULAI JAGUNG DI INDONESIA

Ummu S. Rustiani, Meity S. Sinaga, Sri Hendrastuti Hidayat, Suryo Wiyono
10.14203/beritabiologi.v14i1.1860
| Abstract views: 4412 | PDF views: 4148

Abstract

Downy mildew is very detrimental disease of maize production in Indonesia. Adequate information regarding the identification key based on morphological and morphometric characteristic of the causal fungi of maize downy mildew in Indonesia is limited. Study for detection and identification of morphological, morphometric, and moleculer base is urgently required. Artificial sporulation induction method performed to obtain the morphology of the fungus as a whole.The fungi were morphologically identified as symptomatic maize downy mildew collected from 13 provinces in Indonesia.Three species, namely P. maydis, P.sorghi, and P. philippinensis were identified based on the shape,size, and conidial cell wall thickness, size and number of branching coniodiophores, and long sterigmata.Confirmation by PCR succesfully amplified target DNA of P. maydis, P. sorghi, and P. philippinensis. The identification key of third species of Peronosclerospora accurately to be used in identification of Peronosclerospora causes downy mildew of maize in Indonesia.This identification key is recommended as a method of identification of the causal downy mildew of maize in Indonesia.

Keywords

identification key, morphology, morphometric, specific primers.

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References

Ahmad IB, DO Lopez and AM Mahir. 1994. Biology of the

Downy Mildew Pathogen of Corn in Malaysia.Kasetsart Jurnal (Natural Science) 28, 483-488.

Badan Karantina Pertanian [BKP]. 2013. Peraturan Menteri Pertanian tentang Daftar OPTK A1 dan A2 Berikut Penggolongannya, 239. Badan Karantina Pertanian, Jakarta.

Bock CH and MJ Jager. 1996. Downy Mildew on Sorghum.

International Sorghum and Millets Newsletter 37, 33-

Bonde MR, GL Peterson, RG Kenneth, HD Vermeulen,

Sumartini and M Bustaman. 1992. Effect of Temperature on Conidial and Systemic Infection of Maize by Peronosclerospora species. Phytopathology 82, 04-109.

Burhanuddin. 2011a. Proses Sporulasi Peronosclerospora

philippinensis pada Tanaman Jagung. Prosiding Seminar Ilmiah dan Pertemuan Tahunan PEI dan PFI XX Komda Sul-Sel, Makassar 27 Mei 2010. Saenong S (Penyunting), 366-369. Bidang Publikasi dan Seminar ilmiah BALITSEREAL Maros.

Burhanuddin. 2011b. Identifikasi Cendawan Penyebab

Penyakit Bulai pada Jagung Di Pulau Jawa dan Madura. Superman : Suara Perlindungan Tanaman 1, 21-26.

CAB International [CABI]. 2014. Crop Protection Compendium. HYPERLINK "http://www.cabi.org/

cpc"www.cabi.org/cpc. (Diunduh 03 Januari 2014).

Fachrul MF. 2012. Metode Sampling Bioekologi, 198. Edisi ke-1. Bumi Aksara, Jakarta.

Hikmawati, T Kuswinanti, dan MB Pabendon. 2011. Karakterisasi Morfologi Peronosclerospora spp.,

Penyebab Penyakit Bulai Pada Tanaman Jagung, dari

beberapa Daerah di Indonesia. Jurnal Fitomedika 7,

-161.

Huber DM, ME hugh-jones, MK Rust, SR Sheffield, D

Simberloff, CR Taylor, N Gratz, J Mange and HD

Thruston. 2002. Invasive Pest Species: Impact on

Agricultural production, Natural Resources, and The

Environment. Council for Agricultural Science and

Technology (CAST) Issue Paper 20, 1-17.

Muis A, M Pabendon, N Nonci dan WBS Waskito. 2013.

Keragaman Genetik Patogen Penyebab Bulai Berbasis

Marka SSR. Penelitian Pertanian Tanaman Pangan

, 139-147.

Kim SK, NM Yoon, HJ Kim, YB Kim, N Chay, SM Kim, KS

Oeuun, P Bora, N Glaudino and L Fontes. 2007.Severe Epidemics of Downy Mildew (Peronosclerospora sorghi) on Maize in Cambodia, East Timor, and Vietnam. Maize Genetics Cooperation Newsletter 81, 14. HYPERLINK "http://www.cabi.org/cpc"www.cabi.org/cpc. (Diunduh 03

Desember 2014).

Lukman R, A Afiudin and T Luberstedt. 2013.Unrevealing

The Genetic Diversity of Maize Downy Mildew in

Indonesia. Journal of Plant Pathology and Microbiology 4, 162 doi: 10_4172/2157-7471.1000162.

Perumal R, RE Padmavathi, Saradha, N Eun-Gyu, KR Umesh, KP Louis, NO Gary, GL Douglas and WM Clint. 2008. Simple Sequence Repeat Markers Useful for Sorghum Downy Mildew (Peronosclerospora sorghi) and Related Species. BMC Genetics. HYPERLINK "http://www.biomedcentral.com/1471- 2156/9/77" www.biomedcentral.com/1471-2156/9/77. (Diunduh 6 Desember 2012).

Quimio TH. 1981. Plant Pathogenic Fungi: Principles and Taxonomy (a syllabus for pp 224), 111. UP at Los Banos, Manila.

Semangun H. 2008. Penyakit-penyakit Tanaman Pangan di Indonesia. 475. Edisi ke-2. Gadjah Mada University Press, Yogyakarta.

Shivas RG, MJ Ryley, S Telle and JR Liberato. 2013. Peronosclrospora australiensis sp. novo, and P. sargae sp. novo, Two Newly Recognised Downy Mildew in Northern Australia. and Their Biosecurity Implications. Australasian Plant Pathol. doi.10.10007/s1 33 33-4011-0097-x.

Surtikanti. 2012. Penyakit Bulai pada Tanaman Jagung. Didalam: Saenong S, editor. Prosiding Seminar Ilmiah dan Pertemuan Tahunan PEI dan PFI XXI Komda SulSel, Makassar 27 Desember 2011, Saenong S. (Penyunting), 41-48. Bidang Publikasi dan Seminar Ilmiah BALITSEREAL Maros.

Ulloa M and RT Hanlin. 2000. Illustrated Dictionary of Micology, 448. Edisi ke-1. APS Pr, New York.

Wakman W. 2006. Penyebab Penyakit Bulai Pada Tanaman Jagung, Tanaman Inang Lain, Daerah Sebaran, dan Pengendaliannya. Prosiding Seminar Ilmiah dan Pertemuan Tahunan PEI dan PFI XVI Komda Sul-Sel.Makassar 23 Mei 2005. Saenong S. (Penyunting), 36-47. Bidang publikasi dan Seminar ilmiah BALITSEREAL Maros.

White DG. 2000. Compendium of Corn Diseases. Third Edition, 78. APS Press, Minnesota. Xu H, S Qiang, P Genovesi, H Ding, J Wu, L Meng, Z Han, J Miao, B Hu and J Guo. 2012. An Inventory of Invasive Alien Species in China. Neo Biota 15, 1-26.doi:10.3897/neobiota.15.3575.

DOI: https://doi.org/10.14203/beritabiologi.v14i1.1860

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