KARAKTERISASI BAKTERI PENGHASIL ASAM INDOL ASETAT DAN PENGARUHNYA TERHADAP VIGOR BENIH PADI

Puji Lestari, Yadi Suryadi, Dwi Ningsih Susilowati, Tri Puji Priyatno, I Made Samudra
| Abstract views: 363 | PDF views: 717

Abstract

The ability to produce indole acetic acid (IAA) by endophytic bacteria is one of the basic criteria for the use of bacteria as plant growth promoter agent which is essential for the agricultural production.The objectives of this study were to evaluate the ability of 17 bacterial isolates to produce IAA and its effect on improvement of rice seed germination and molecular identification of the selected isolates based on the 16S rRNA gene. The IAA content was determined using Salkowski method measured by spectrophotometer UV-Vis and the effect of endophytic bacteria inoculation on seed germination was done by in vitro assay. Sequences of the selected isolates 16S rRNA amplified by PCR were analyzed the homology against bacterial 16S rRNA database in Genebank. IAA values ranged from 6.632 to 50.053 mg/L with the highest IAA production shown by isolate 6KJ which was followed by 4PB (41.807 mg/L). Bacterial IAA increased rice seed vigor significantly compared to control. However, bacterial inoculation with different concentrations of IAA did not significantly affect the growth of rice plants. Based on the IAA and its effect on seed vigor, 6KJ, 4PB and 2KB were selected for molecular identification. Results showed that the three isolates belonged to Bacillus genus, 6KJ as B. aryabhattai, 4PB belonging to B. cibi and 2KB having 97% homology with B. marisflavi. Further evaluation of the selected endophytic isolates producing IAA is necessary to be carried out to explore their potency as a source of hormone to promote plant growth.

Keywords

indole acetic acid, endophytic bacteria, rice, vigor.

Full Text:

PDF

References

Abdul-Baki AA and JD Anderson. 1973. Vigor determination in soybean by multiple criteria. Crop Science 13, 630-633.

Ahmad F, I Ahmad and MS Khan. 2005. Indole acetic acid production by the indigenous isolates of Azotobacter and fluorescent Pseudomonas in the presence and absence of tryptophan. Turkish Journal of Biology 29, 29-34.

Amann RI, W Ludwig and KH Schleifer. 1994. Identification of un-cultured bacteria: A challenging task for molecular taxonomists. American Society for Microbiology News 60, 360-365.

Bartel B. 1997. Auxin biosynthesis. Annual Review of Plant Physiology and Plant Molecular Biology 48, 51-66. Bialek K, L Michalczuk, and JD Cohen. 1992. Auxin biosynthesis during seed germination in Phaseolus vulgaris. Plant Physiology 100, 509-517.

Botero LM, SD Imperio, M Burr, TR McDermott, M Young and DJ Hassett. 2005. Poly (A) polymerase modification and reverse transcriptase PCR amplification of environmental RNA. Applied and Environmental Microbiology 71, 1267-1275.

Glickmann E and Y Dessaux. 1994. A critical examination of the specificity of the Salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Applied and Environmental Microbiology 61, 793-796.

Grimont PAD, M Vancanneyt, M Lefévre, K Vandemeulebroecke. L Vauterin, R Brosch, K Kersters, and F Grimont. 1996. Ability of biology and Biotype-100 systems to reveal the taxonomic diversity of the pseudomonas. Systematic and Applied Microbiology 19, 510-527.

Hartung JS. 1998. Molecular probes and assays useful to identify plant pathogenic fungi, bacteria, and marked biocontrol agents. In: Boland, GJ and Kuykendall, LD (eds) Plant microbe interactions and biological control. Marcel Dekker, Inc. New York, Basel and Hongkong. 393-413.

Idris EE, DJ Iglesias, M Talon, and R Borriss. 2007. Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42. Molecular Plant- Microbe Interaction 20, 619-626.

Karnwal A. 2009. Production of indole acetic acid by fluorescent Pseudomonas in the presence of L-tryptophan and rice root exudates. Journal of Plant Pathology 91, 61- 63.

Kim KY, MH Ko, and H Liu. 2013. Phylogenetic relationships of Pseudorasbora, Pseudopungtungia, and Pungtungia (Teleostei; Cypriniformes; Gobioninae) inferred from multiple nuclear gene sequences. BioMed Research International 3, 1-6.

Krimitzas A, I Pyrri, VN Kouvelis, E Kapsanaki-Gotsi, and M A Typas. 2013. A phylogenetic analysis of greek isolates of Aspergillus species based on morphology and nuclear and mitochondrial gene sequences. BioMed Research International. 18, 1-18.

Lazo G, RR Roffey, and DW Gabriel. 1987. Pathovars of Xanthomonas campestris are distinguishable by restriction fragment-length polymorphism. International Journal of Systematic and Evolutionary Microbiology 373, 214-221.

Leveau JH and SE Lindow. 2004. Utilization of plant hormone indole-3-acetic acid for growth by Pseudomonas putida Strain 1290. American Society of Microbiology 5, 2365 - 2370.

Lestari P, DN Susilowati and EI Riyanti. 2007. Pengaruh hormon asam indol asetat yang dihasilkan Azospirillum sp. terhadap perkembangan akar padi. Jurnal AgroBiogen 2, 66-72.

Ludwig W and KH Schleifer. 2002. Bacterial phylogeny based on 16S and 23S rRNA sequence analysis. FEMS Microbiology Review 15, 155-173.

Manuel AE, EL Periago, EM Carballo, JS Gándara, JC Mejuto and LG Río. 2008. The mobility and degradation of pesticides in soils and the pollution of groundwater resources. Agriculture, Ecosystems & Environment 123, 247-260.

Marchesi JR, T Sato, AJ Weightman, TA martin, JC Fry, SJ Hiom, D Dymock and WG Wade. 1998. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Applied and Environmental Microbiology 64, 795-799.

Maslahat M and Suharyanto. 2005. Produksi indole acetic acid oleh bakteri yang diisolasi dari akar tanaman karet (Hevea brasiliensis). Jurnal Nusa Kimia 5, 26-35.

Minamisawa K, KI Ogawa, H Fukuhara, and J Koga. 1996. Indole pyruvate pathway for indole-3-aceric acid biosynthesis in Bradyrhizobium elkanii. Plant and Cell Physiology 37, 449-453.

Mohite B. 2013. Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. Journal of Soil Science and Plant Nutrition 13, 638-649.

Nobandegani MBJ, HM Saud, and WM Yun. 2015. Phylogenetic relationship of phosphate solubilizing bacteria according to 16S rRNA genes. BioMed Reearch International 5, 1-5.

Overvoorde P, H Fukaki and T Beeckman. 2010. Auxin control of root development. Cold Spring Harb. Perspective in Biology 2, a001537.

Patten CL and BR Glick. 2002.Role of Pseudomonas putida in dole acetic acid in development of the host plant root system. Applied and Environmental Microbiology 68, 3795-3801.

Rahman A, A Bannigan, W Sulaman, P Pechter, EB Blancaflor and TJ Baskin. 2007. Auxin, actin and growth of the Arabidopsis thaliana primary root. The Plant Journal 50, 514-528.

Rajapaksha RMCP, MA Tobor-Kapon and E Bååth. 2004. Metal toxicity affects fungal and bacterial activities in soil differently. Applied and Environmental Microbiology 70, 2966-2973.

Rubio MGT, SAV Olata, JB Castillo and PM Nieto. 2000. Isolation of Enterobacteria, Azotobacter sp. and Pseudomonas sp., producers of indole-3-acetic acid and siderophores, from Colombian rice rhizosphere. Revista Latinoamericana de Microbiología 42, 171-176.

Ryu CM, MA Farag, CH Hu, M Reddy, HX Wei, PW Paré, and J Kloepper. 2003. Bacterial volatiles promote growth in Arabidopsis. Proceeding of the National Academy of Science. United States of America 100, 4927-4932.

Sahasrabudhe MM. 2011.Screening of rhizobia for indole acetic acid production. Annals of Biological Research 2, 460-468. Sarwar M and WT Frankernberger. 1994. Tryptophan dependent biosynthesis of auxin in soil. Plant and Soil 160, 97-104.

Shahab S, N Ahmed and NS Khan. 2009. Indole acetic acid production and enhanced plant growth promotion by indigenous PSBs. African Journal of Agricultural Research 4, 1312-1316.

Spaepen S, J Vabderleyden and R Remans. 2007. Indole-3- acetic acid in microbial and microorganism-plant signaling. FEMS Microbiology Review 31, 425-448.

Widiastuti H, Siswanto and Suharyanto. 2010. Karakterisasi dan seleksi beberapa isolat Azotobacter sp. untuk meningkatkan perkecambahan benih dan pertumbuhan tanaman. Buletin Plasma Nutfah 16, 160-167.

Wright AD, MB Sampson, MG Neuffer, L Michalczuk, JP Slovin and JD Cohen. 1991. Indole-3-acetic acid biosynthesis in the mutant maize orange pericarp, a tryptophan auxotroph. Science 254, 998-1000.


Refbacks

  • There are currently no refbacks.