POTENSI EKSTRAK PEGAGAN (Centella Asiatica) DAN KUNYIT (Curcuma longa) UNTUK MENINGKATKAN AKTIVITAS ENZIM GLUTATION PEROKSIDASE (GSH Px) PADA JARINGAN HATI TIKUS

Abstract

This experiment was designed to study the effect of pegagan (Centella asiatica) and turmeric (Curcuma longa) extracts on the activities of glutathione peroxidase (GSH- Px) in liver of white Spraque Dawley rat aged 2 months old with average weight of 200 grams. Pegagan was extracted using infuse method, and turmeric was extracted using dekokta method. The activity of GSH-Px was measured using Flohe and Gunzler’s methods. Extract pegagan and turmeric were assumed to have both preventive and curative treatments. For the preventive treat- ment, extract pegagan and turmeric at concentrations of 22.05 mg/ml : 184.1 mg/ml could increased the activity of GSH-PX enzyme, i.e. 232.60 ± 21.40 mU / mgprotein. This was approximately 79% than the levels of GSH- Px enzyme in normal liver without paracetamol which was 190.78 ± 9.28 mU/mgprotein. For the curative treatment, extract pegagan and tumeric at concentration of 22.05 mg/ml : 184.1 mg/ml could improved GSH- Px enzyme activity (i.e. 239.01 ± 47.40 mU/ mgprotein). This figure was about 92% higher than the levels of GSH-Px enzyme in the liver normal without paracetamol which was 190.78 ± 9.28 mU/mg protein. Pegagan and turmeric extracts with a high concentration could increased the activity of GSH-Px enzyme, thus potential as curative treatment.