CHARACTERISTIC OF CMCASE OF Bacillus sp. ISOLATED FROM SOIL OF GUNUNG HALIMUN NATIONAL PARK*[Karakteristik CMCase Bacillus sp. Yang Diisolasi dari Tanah Taman Nasional Gunung Halimun]]

I Made Sudiana
10.14203/beritabiologi.v6i1.1180
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Abstract

Delapan isolat bakten diisolasi dari lanah Taman Nasional Gunung Halimun dipelajari posisi laksonomi dan karaktcr fisiuloginyu. Kemanipuan sclululilik dari 8 isolat Icrscbut diuji dengan membandingkan luas koloni dan zona bening yang terbentuk pada koloni yang ditumbuhkan pada CMC.Selanjutnya Karakteristik CMC-ase dari satu isolat yang mempunyai kemampuan sclulolitik yang paling tinggi diuji. Isolat dengan cepal menggunakan CMC scbagai sumber carbon utama pada suhu 30° C dengan keeepatan pertumbuhan 1.71 '. 1,58"',dan 1.50 ' pada pH ft. 7 dan 8. Tidak ada pertumbuhan yang bcrarti pada kultur yang nienggunakan Avicel sebagai sumber karbon utama.Aktivitas CMCase maksiinum sebcsar6 Unit terjadi pada pH 7 setclah 4 hari kultivasi. Maksimum pertumbuhan scl dicapai pada hari ke 4. Penambahan gliikosa incnstimulusi pertumbuhan binmassa tctatpi menghambat aklivitas enzim. Km dan Vmuk dari CMC-ase adalah IDmM dan2.(,8mM.d'.

Keywords

Baklcri selulolilik. Bacillus sp., Taman Nasional Ouming Halimun.

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References

Alexander, M. 1961. Introduction to Soil Microbiology. John Wiley and Sons, Inc. Him 169.

Belaich JP, Tardif C, Belaich A, Gaudin C. 1997. The cellulolytic system of Clostridium cellulolyticum. JBiotechnol 57, 3-14.

Blackall LL, Hayward AC, Sly LI. 1985. Cellulolytic and extremophilic Gramnegative bacteria: revival of the genus Cellvibrio. JAppl Bacteriol 59, 81-97.

Coughlan MP, Mayer F. 1992. The cellulosedecomposing bacteria and their enzyme systems. In: The Prokaryotes: a Handbook on the Biology of Bacteria. 2nd edn. Balows A, Truper HG, Dworkin M, Harder W and Schleifer KH (Eds.). Springer-Verlag, New York. Him 460-516.

Dees C, Ringelberg D, Scott TC, Phelps TJ. 1995. Characterization of the cellulose degrading bacterium NCIMB 10462. Appl. Bpchem Biotechnol 51, 263-274.

Elberson MA, Malekzadeh F, Yazdi MT, Kameranpour N, Noori-Dloii MR, Matte MH, Shahamat M, Colwell RR, Sowers KR. 2000. Cellulomonas persica sp. nov. and Cellulomonas iranensis sp. nov., mesophilic cellulose-degrading bacteria isolated from forest soil. Int J System Evol Microbiol 50, 993-996.

Enari, TM. 1983. Microbial cellulase. Dalam: Microbial Enzymes and Biotechnology. Fogarty WM (Ed). Him 183-223.

Gal L, Pages S, Gaudin C, Belaich A, Reverbelleroy C, Tardif C, Belaich JP. 1997. Characterization of the cellulolytic complex (cellulosome) produced by Clostridium cellulolyticum. Appl. Environ Microbiol 63, 903-909.

Hatano, T., Mutsuko K, Zhifeng C, Meiko K, Tokichi M, and Sakuzo F. 1991. Purification and characterization of a carboxymethylcellulose degrading enzyme secreted by a yeast strain newly isolated from soil. Journal of Fermentation and Bioengineering 71, 313-317.

Hiroki M and Watanabe MM. 1996. Microbial community and rate of cellulose decomposition in peat soils in a mire. Soil Sci Plant Nutr. 42,893-903.

Joson,LM and Coronel LM. 1986. Isolation, screening and characterisation of celluloseutilizing bacteria. The Philip.J. Sci. 3, 223-226.

Kim CH. 1995. Characterization and substrate specificity of an endo-G-l,4-D-glucanase I (Avicelase I) from an extracellular multienzyme complex of Bacillus circulans. Appl Environ Microbiol 61, 959-965.

Madigan MT, Martinko and Parker J. 2000. Brock Biology of Microorganism. Prentice Hall, New Yersey. Him 185-199.

Nakase T, Matofumi S, Masako T, Makiko H, Takushi H and Sakuzo F. 1994. A taxonomic study on cellulolytic yeasts and yeast-like microorganisms isolated in Japan I. Ascomycetous yeasts genera Candida and Williopsis, and a yeast-like genus Prototheca. J. Gen. Appl. Microbiol 40, 519-531.

Shoham Y, Lamed R and Bayer EA. 1999. The cellulosome concept as an efficient microbial strategy for the polysaccharide degradation of insoluble polysaccharides. Trends Microbiol 7,275-281.

DOI: https://doi.org/10.14203/beritabiologi.v6i1.1180

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