CELLULOLYTIC BACTERIA OF SOIL OF GUNUNG HALIMUN NATIONAL PARK

I Made Sudiana, Ritai Dwi Rahayu, Hartati Imanuddin, Maman Rahmansyah
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Abstract

The population of aerobic cellulolyitic bacteria (ACB) of soil Gunung Halimun National park and its celulolytic capacity were studied.The soil samples were collected from various altitude (500-1500) m asl.Microbial isolation was performed by culture enrichment technique with CMC (carboxymethyl cellulose) as the major carbon sources.The quantitative determination of ACB was performed by growing the microbes on CMC containing media, and utilizing congored as an indicator.ACB was indicated by formation of clearing zone surrounding growing colony.Cellulytic capacity of each isolates was determined by analysing the ratio of colony and clear zone formation.ACB were quite heterogenous include Bacillus sp., Clostridium sp., Chromobacterlum sp., Enterobacter sp., Moraxella sp. and Pseudomonas sp.

Keywords

Culturable aerobic cellulolytic bacteria, Gunung Halimun National Park.

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References

Alexander M. 1961. Introduction to Soil Microbiology. John. Wiley & Sons, INC. London. Page 163.

Anderson JPE and Domsch KH. 1978. A Physiological Method for the Quantitative Measurement of Microbial Biomass in Soils. Soil Biol Biochem 10, 215-221

Bossio DA, Scow KM, Gunapala N and Graham KJ. 1998. Determinants of Soil Microbial Communities: Effects of Agricultural Management, Season, and Soil Type on Phospholipid Fatty Acid Profiles. Microb Ecol 36, 1-12

Chandler DP, Li SH, Spadoni CM, Drake GR, BalkwiU DL, Fredrickson JK, and Brockman FJ. 1997. A Molecular Comparison of Culturable Aerobic Heterotrophic Bacteria and 16S rDNA Clones Derived from a Deep sub Surface Sediment. FEMS Microbiol Ecol 23, 131—144

Coughlan MP, Mayer F. 1992. The Cellulose- Decomposing Bacteria and Their Enzyme Systems. Dalam The Procaryotes, 2nd edition. Balows A, Truper HG, Dworkin M, Harder W, Schleifer KH (Editor) Springer Verlag, New York, page 460-515.

Distel DL, DeLong EF and Waterbury JB. 1991. Phylogenetic Characterization and in Situ Localization of the Bacterial Symbiont of Shipworms (Teredinidae: Bivalvia) by Using 16S rRNA Sequence Analysis and Oligodeooxynucleotide Probe Hybridization. Appl Environ Microbiol 57, 2376-2382.

Doran JW, and Parkin TB. 1996. Quantitative Indicators of Soil Quality: a Minimum Data set. Dalam Methods for Assessing Soil Quality. Doran JW, Jones AJ (Editor) SSSA Special Publ. No 49. Madison, WI, him 25-37

Eriksson KEL, Blanchette RA, Ander P. 1992. Microbial and Enzymatic Degradation of Wood and Wood Components, Ch.2.6: Cellulose degradation by bacteria. Springer Verlag, Berlin, him 137-158

Heinemeyer O, Insam H, Kaiser EA, Walenzik G. 1989. Soil Microbial Biomass and Respiration Measurements: an Auto-mated Technique Based on Infra-red gas Analysis. Plant Soil 116, 191-195

Hiroki M, and Watanabe MM. 1996. Microbial Community and Rate of Cellulose Decomposition in Peat Soils in a Mire. Soil Sci Plant Nutr 42, 893-903

Kennedy AC, Gewin VL. 1997. Soil Microbial Diversity: Present and Future Considerations. Soil Sci. 162, 607-617

Mullings R, and Parish JH. 1984. Mesophilic Aerobic gram Negative Cellulose Degrading Bacteria from Aquatic Habitats and Soils. J Appl Bacteriol 57, 455-468

Rose AH. 1980. Microbial Enzymes and Bioconversion. Academic Press him 283-326.

Ulrich S and Wirth W. 1999. Phylogenetic Diversity and Population Densities of Culturable Cellulolytic Soil Bacteria Across an Agricultural. Encatchment Microb. Ecol. 37,238-247.


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